Corporate Banner
Satellite Banner
LIMS & ELNs
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

Doping Evaluation with NuGenesis SDMS and TargetLynx

Published: Tuesday, November 16, 2010
Last Updated: Tuesday, November 16, 2010
Bookmark and Share
The Doping Control Lab of the Karolinska University Hospital in Stockholm, Sweden is one of 33 doping control labs worldwide accredited by the World Anti-Doping Agency (WADA). The lab’s nine chemists and lab technicians analyze approximately 7,500 samples annually. Managing information and controlling the flow of data were major challenges. Enhancing the lab’s information and knowledge management processes is an area where Waters Laboratory Informatics has played a crucial role.

The Doping Control Lab of the Karolinska University Hospital in Stockholm, Sweden is one of 33 doping control labs worldwide accredited by the World Anti-Doping Agency (WADA). The lab’s nine chemists and lab technicians analyze approximately 7,500 samples annually, including 4,717 athlete samples (compared to 183,337 samples worldwide). 87 positive samples were found at the Karolinska lab in 2006 (3,909 worldwide), using hyphenated chromatographic techniques such as GC/MS, GC/NPD, and LC/MS/MS.

Karolinska’s Doping Control Lab receives samples from a variety of customers, including the Swedish Sports Confederation, WADA, and other International sports confederations, as well as from correctional institutions and Public Health services.

The Doping Laboratory is primarily financed by payments for analyses performed, therefore the more samples that are run and results secured, the more income is generated to finance research and extended testing.

CHALLENGE

The WADA’s World Anti-Doping Code provides an internationally standardized list of prohibited substances for athletes both in and out of competition, as well as some specific regulations for particular sports disciplines. A laboratory’s work is assessed by WADA on the basis of a “proficiency testing program”, which must be successfully completed if the laboratory is to retain its status as an official doping control laboratory.

The Karolinska lab focuses specifically on testing for the 44 diuretics and other masking agents, as well as the 58 stimulants listed in the WADA code. In the future, they are planning to add tests for narcotics and beta-blockers to their spectrum.

Tests for diuretics and narcotics have been conducted using GC/MS, involving complex sample preparation with hydrolyzation, extraction, and derivatization. Stimulant tests using GC/NPD involve similarly complex preparation steps.

Increasing regulatory pressure and introduction of new technologies have driven up the number of samples analyzed and increased the quality of data produced.

Managing information and controlling the flow of data were major challenges. Enhancing the lab’s information and knowledge management processes is an area where Waters Laboratory Informatics has played a crucial role.

THE SOLUTION

New analytical methods have been introduced using LC/MS/MS. The sample preparation for this method is significantly easier, since the diluted urine sample can be directly injected into the instrument with an internal standard added. This allows for more samples to be prepared and analyzed.

The solution chosen by the Karolinska Doping Control Lab is the Waters® ACQUITY UPLC® System with a BEH shield RP18 column, 50 x 2 mm, 1.7 μm, with a flow rate of 0.4 mL/min (MeOH/NH4OAc 10 mM gradient, 6 minutes). A QC-sample is run at every 12th injection; water and urine are used as blanks. For detection, Waters’ Quattro Premier™ XE tandem quadrupole Mass Spectrometer is operated in MRM mode. The instruments are controlled by Waters’ MassLynx™ Software, with processing and reporting done using the TargetLynx™ Application Manager. TargetLynx is specifically designed for quantitation and specific analyte confirmation and it streamlines and automates the analysis of these samples.

All raw instrument data are automatically and securely captured and stored within Waters’ NuGenesis® Scientific Data Management System (SDMS).

The Karolinska lab has found that with the UPLC® System, they can now screen up to 105 analytes in eight minutes, injection to injection.

The hospital uses TargetLynx Application Manager to find analytes that comply with three criteria:

•    Correct retention time
•    Above signal-to-noise
•    Above concentration threshold

If a positive hit of an illegal drug is detected, TargetLynx will flag this analyte.

Having automated the analysis and post analysis calculations, they were still faced with the need to manually review 140 compounds per sample and determine the positive hit patterns. Today, there are more than 100 samples in each batch. This manual process is tedious and time-consuming, and mistakes can easily be made. Most samples are negative (only 2.5% of samples are positive).

The next step was to automate and streamline the TargetLynx report utilizing the print capture capabilities within SDMS.

The analyst now prints all reports into SDMS with a TargetLynx format, that include the stimulants, ephedrines, and diuretics as individual reports. These reports are normally several hundred pages long.

When the analyst runs the custom application provided by the Waters Enterprise Software Services Group, they have the choice of defining the batch number by parameters that include standards, controls, and blanks. Then all reports can be extracted from a particular batch, excluding all standards, QC’s, and internal control analytes. QC results are automatically exported to their LIMS.

The end result is an SDMS report of analytes that were found, detailed by batch number and name of compound. Within this “hit list”, the user can click a link and SDMS will output the printed TargetLynx report that is stored within SDMS. Analysts can review the report just as they always have, but now they only have to concentrate on verifying the true positive hits (approximately 187 per year) instead of reviewing all 7,500 reports.

BUSINESS BENEFIT

The Waters solution is providing a number of benefits to the Karolinska University Hospital’s Doping Control Laboratory operations including:

•    Increased revenue potential – greater sample throughput with UPLC/MS/MS.
•    Greater productivity – approximately two hours are saved per batch in reviewing results for illegal compounds with SDMS and TargetLynx reports.
•    Reducing risk of data loss – SDMS automatically manages raw data backups from all LC/MS instruments.
•    Better data accessibility – all reports and raw data are fully text searchable from within SDMS, reducing the time to locate critical information.
•    Connecting science to the business of the hospital – TargetLynx and SDMS have automated and streamlined results calculations, review, and consolidated reporting.


Further Information
Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,500+ scientific posters on ePosters
  • More than 3,700+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

New Solutions to Old Challenges
Waters Corporation introduces a new NuGenesis Lab Management System (LMS) that integrates sample management functions and business operations across the enterprise. Technology Networks' Editor Helen Gillespie interviewed Waters' Garrett Mullen for details.
Tuesday, July 01, 2014
Waters and DANI Instruments Announce Integrated and Compatible Network
Waters Empower Software Now Works Seamlessly with DANI Headspace and GC Instruments
Saturday, April 06, 2013
Scientific News
Exploring Living cells
JPK reports the exploration of living cells using nanoscale and single molecule techniques through the application of scanning probe microscopy.
The Perfect Partnership: Research & Industry; Software & Instrumentation. It really starts to come together at ASMS 2015
Collaboration and knowledge-sharing were evident everywhere: on the bus, in the hallways and in the bars. This article aims to capture this theme and share with you some of the fruits of this coming together of science and industry.
Designing New Pain Relief Drugs
Researchers have identified the molecular interactions that allow capsaicin to activate the body’s primary receptor for sensing heat and pain, paving the way for the design of more selective and effective drugs to relieve pain.
Antibody Pries Loose Bacteria’s Grip
Study finds novel method of improving antibody efficacy.
CLAIRE Brings Electron Microscopy to Soft Materials
CLAIRE works by essentially combining the best attributes of optical and scanning electron microscopy into a single imaging platform.
Sample & Analysis Tracking in Oncogenomic Experiments
The study outlines Onco-STS, a web-based laboratory information management system for sample and analysis tracking in oncogenomic experiments.
Pittcon Announces Wide Variety of Short Courses
Courses range from beginner and intermediate to advanced levels.
Ultra-enriched Silicon Paves the Road to Quantum Computing
Using a relatively straightforward technique, a team of NIST researchers has created what may be the most highly enriched silicon currently being produced.
BioClinica and Rutgers University Announce Research Project Results
Scientific Partnership Aims to Expand Automation of Clinical Trial Image Data Analysis
Leading Japanese CRO Standardizes on Thermo Scientific Watson LIMS
JCL Bioassay uses Thermo Scientific Watson LIMS to optimize communication, data management and quality control during U.S. expansion.
SELECTBIO

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,500+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,700+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FREE!