This poster compares the volumes of sample solutions delivered by an array of stainless steel and hydrophobic-coated pins as measured by a fluorescent-dye approach versus a dual-dye absorbance approach.

True Point of Care medical devices place unique demand on micropump characteristics. SFC Fluidics ePump is an innovative electrochemical pumping system that is different from other microfluidic pumps.

This poster demonstrates cyclic liquid chromatography on chip and shows how this can be used in other closed loop applications.

Two one time use valve strategies have been successfully demonstrated in a completely sealed assay chip. They were also shown to be feasible for on chip reagent storage.

Microfluidic electrochemical biosensor based on Loop-mediated Isothermal Amplification (LAMP) was developed for detection and quantification of E.coli. The electrochemical detection was achieved using Hoechst 33258 redox molecule and linear sweep voltametry (LSV). Here, we detect and quantify 48 CFU/ml of bacteria in 35 min in LB media and urine samples. This microfluidic chip has great potential to be used as a point of care diagnostic (POC) device for clinical application.

In this work a silicon biochip based on a matrix of transparent micro-reactor sites coupled with a linear SPAD (Single-Photon Avalanche Diode) detector array for detecting traces of proteins in biological fluids is presented. A microfluidic layer of PDMS containing a peristaltic pump was implemented. The biofunctional layer for the detection of target proteins is based on a dual-site binding strategy employing DNA aptamers.

SpectroSens, a multi-channel optical microchip sensor system suitable for rapid, label-free multiplexed detection of a wide range of bio-hazardous agents is presented. Optical chips containing multiple high-precision planar Bragg gratings are exploited as low-cost, robust refractive index sensors.

In this work the development of microfluidics devices using a simple, fast and low cost fabrication method is shown. The devices were made using a photopolymer designed for making stamps. The obtained results make this concept a convenient technology for configuring compact service areas of specialized microfluidic and detection microstructures compatible with autonomous LOC devices.

A PDMS lab-on-a-chip for one step DNA isolation and real time-polymerase chain reaction (RT-PCR) has been designed, fabricated, and characterized for point-of-care clinical diagnostics. In addition, a module for on-chip optical detection based on SPAD - Single-Photon Avalanche Diode - detector has also been developed and used to monitor the presence of specific DNA polymorphisms possibly related to genetic diseases.

A microfluidic mixer for the purpose of pulse experiments is designed, fabricated, and characterized. The performance of the micromixer was successfully validated by means of chemical iodemetry. Experiments show that the system is suitable for providing a controlled feeding pulse to a cell population without compromising the viability of eukaryotic cells. The micromixer could be further integrated with other microfluidic components for cell lysis and separation on one chip.