|Real Time Dual-Channel Multiplex SERS Ultradetection|
Sara Abalde-Cela,†,‡ Chris Abell,† Ramón A. Álvarez-Puebla, ?,§ and Luis M. Liz-Marzán‡,?,#
Real time On-line multiplex SERS detection in a microfluidic device.
|Flow Enhancement and Dynamic of Microfluidic Base Biosensor |
Esmail Abdullah Mohammed Basheer, Hayder A.Abdul Bari
The design of new biosesnor base on microfluidic system is presented. The design is consist of two chips, Microchannels chip and electrodes chip. By varying the size and optimizing the microchannels and electrode, the sensitivity of the biosensor will increase.
|Novel Approaches to High-throughput Measurements of Replicative Lifespan in Yeast by Microfluidic Size Sorting and Genetic Engineering|
Ilka Lewrenz, Stephanie Wälter, Jacqueline Franke
Development of a high-throughput screen utilizing a microfluidic device and a linearly growing yeast strain to simultaneously test the influence of various drugs on the replicative lifespan of yeast.
|Significant Increase of Sensitivity on Rapid Influenza Antigen Assays Using Silver Amplification Immunochromatography.|
Satoshi KIMURA1), Hideyoshi OHTO2), Hayato YAMAGUCHI1), Seiji FUKUOKA1), Emiko NAKAMA1), Akiko TERADA3), and Yoh UMEDA2)
We have developed a new rapid bed-side assay system for in vitro diagnosis of infectious diseases, using “silver amplification method”, an application of photography-developing technology. The automatic analyzer brings results in fifteen minutes, enhancing sensitivity up to 1000 times compared to the conventional immunochromatography. We applied the technology for diagnosis of hospitalized children. The analyzer was useful for rapid and precise diagnosis of influenza A and B.
|Low-cost Electrochemical Microfluidic Immunoarrays for Cancer Diagnostics|
Brunah A. Otieno, Colleen E. Krause, Gregory W. Bishop, James F. Rusling
This poster describes the development and validation of a simple, low-cost, semi-automated microfluidic device for cancer biomarker protein detection to aid in cancer diagnosis and therapy monitoring.
|Design and Evaluation of High Definition Probe for HPV genotyping Microarray|
Sihn Ae Lee, Ah Reum Park, Inyoung Kim, Ji Hyung Lee, and Jongwon Kim
To improve the sensitivity and specificity of the HPV DNA Microarray, we adopted triple oligonucleotide probes for each targets and selected these probes not to have higher similarity of 75% with each others. These triple probes have shown 10 ~ 100 times higher sensitivities with comparable specificities than the conventional HPV DNA microarray of single oligonuclotide probe.
|Low Cost EIS Methodology to Characterize Microelectrode Arrays used in Biological Applications|
C. A. Santos *, M. A. Flores*, O. Pilloni* , J. Mireles Jr**., A. Falcón ***, T. Fiordelisio ***, L. Oropeza-Ramos*
Diagnostics of electrochemical impedance in micro electrodes for biological potentials measurements is essential. A low cost EIS methodology to characterize microelectrode arrays in contact with cellular medium is presented, and results are validated by comparing them to a commercial system.
|Micro array of Ag-AgCl electrodes for cellular stimulation and sensing |
O. Pilloni*, M. A. Flores*, C. A. Santos*, A. Torres**, J. García**, H. González**, L. Oropeza-Ramos*.
Silver-Silver Chloride is investigated as a fabrication material of planar microelectrodes for sensing and stimulation of excitable cells. A theoretical design method is presented. The platform was tested for different excitable tissue confirming its feasibility.
|A Novel Approach Toward Microfluidic Drug Metabolite Synthesis – Electrosynthetic Methodology Simulating Cytochrome (CYP450) Oxidation|
Romain Stalder, Gregory P. Roth and Philip Podmore
A novel microfluidic technology and electrochemical synthesis method is demonstrated for the efficient generation of known drug metabolites. These metabolites are typically generated on first pass hepatic oxidation in vivo. The FLUX Module, a new microfluidic electrochemical cell manufactured by Syrris Ltd., has been employed to generate the metabolites of five commercial drugs: Tolbutamide, Chlorpromazine, Diclofenac, Primidone and Albendazole.
|On-chip quantification of miRNA using digital droplet PCR|
Q. Cai1, R.S. Wiederkehr1, B. Jones1, B. Majeed1, T. Stakenborg1, P. Fiorini1, L. Lagae1, M Tsukuda2, T. Matsuno2, I. Yamashita2
miRNAs have a great potential in diagnostics. Hence, automated profiling of miRNAs are of great interest. In-house technology show that it is possible to implement a multiplexing assay for miRNAs on a microfluidic chip using digital droplet PCR.
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