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Novel Approaches to High-throughput Measurements of Replicative Lifespan in Yeast by Microfluidic Size Sorting and Genetic Engineering
Ilka Lewrenz, Stephanie Wälter, Jacqueline Franke

Development of a high-throughput screen utilizing a microfluidic device and a linearly growing yeast strain to simultaneously test the influence of various drugs on the replicative lifespan of yeast.

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Significant Increase of Sensitivity on Rapid Influenza Antigen Assays Using Silver Amplification Immunochromatography.
Satoshi KIMURA1), Hideyoshi OHTO2), Hayato YAMAGUCHI1), Seiji FUKUOKA1), Emiko NAKAMA1), Akiko TERADA3), and Yoh UMEDA2)

We have developed a new rapid bed-side assay system for in vitro diagnosis of infectious diseases, using “silver amplification method”, an application of photography-developing technology. The automatic analyzer brings results in fifteen minutes, enhancing sensitivity up to 1000 times compared to the conventional immunochromatography. We applied the technology for diagnosis of hospitalized children. The analyzer was useful for rapid and precise diagnosis of influenza A and B.

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Low-cost Electrochemical Microfluidic Immunoarrays for Cancer Diagnostics
Brunah A. Otieno, Colleen E. Krause, Gregory W. Bishop, James F. Rusling

This poster describes the development and validation of a simple, low-cost, semi-automated microfluidic device for cancer biomarker protein detection to aid in cancer diagnosis and therapy monitoring.

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Design and Evaluation of High Definition Probe for HPV genotyping Microarray
Sihn Ae Lee, Ah Reum Park, Inyoung Kim, Ji Hyung Lee, and Jongwon Kim

To improve the sensitivity and specificity of the HPV DNA Microarray, we adopted triple oligonucleotide probes for each targets and selected these probes not to have higher similarity of 75% with each others. These triple probes have shown 10 ~ 100 times higher sensitivities with comparable specificities than the conventional HPV DNA microarray of single oligonuclotide probe.

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Low Cost EIS Methodology to Characterize Microelectrode Arrays used in Biological Applications
C. A. Santos *, M. A. Flores*, O. Pilloni* , J. Mireles Jr**., A. Falcón ***, T. Fiordelisio ***, L. Oropeza-Ramos*

Diagnostics of electrochemical impedance in micro electrodes for biological potentials measurements is essential. A low cost EIS methodology to characterize microelectrode arrays in contact with cellular medium is presented, and results are validated by comparing them to a commercial system.

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Micro array of Ag-AgCl electrodes for cellular stimulation and sensing
O. Pilloni*, M. A. Flores*, C. A. Santos*, A. Torres**, J. García**, H. González**, L. Oropeza-Ramos*.

Silver-Silver Chloride is investigated as a fabrication material of planar microelectrodes for sensing and stimulation of excitable cells. A theoretical design method is presented. The platform was tested for different excitable tissue confirming its feasibility.

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A Novel Approach Toward Microfluidic Drug Metabolite Synthesis – Electrosynthetic Methodology Simulating Cytochrome (CYP450) Oxidation
Romain Stalder, Gregory P. Roth and Philip Podmore

A novel microfluidic technology and electrochemical synthesis method is demonstrated for the efficient generation of known drug metabolites. These metabolites are typically generated on first pass hepatic oxidation in vivo. The FLUX Module, a new microfluidic electrochemical cell manufactured by Syrris Ltd., has been employed to generate the metabolites of five commercial drugs: Tolbutamide, Chlorpromazine, Diclofenac, Primidone and Albendazole.

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On-chip quantification of miRNA using digital droplet PCR
Q. Cai1, R.S. Wiederkehr1, B. Jones1, B. Majeed1, T. Stakenborg1, P. Fiorini1, L. Lagae1, M Tsukuda2, T. Matsuno2, I. Yamashita2

miRNAs have a great potential in diagnostics. Hence, automated profiling of miRNAs are of great interest. In-house technology show that it is possible to implement a multiplexing assay for miRNAs on a microfluidic chip using digital droplet PCR.

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Droplet Microfluidics for 3D Epithelial Cell Culture
Monika Dolega (Pyzalska), Xavier Gidrol, Nathalie Picollet-D’hahan

The challenge facing 3D cell culture today is to adapt current models to a systems biology approach - in particular, to enable RNA interference-based screens to study the effects of the microenvironment on cellular function.

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Droplet Microfluidics for 3D Epithelial Cell Culture
Monika Dolega (Pyzalska), Xavier Gidrol, Nathalie Picollet-D’hahan

The challenge facing 3D cell culture today is to adapt current models to a systems biology approach - in particular, to enable RNA interference-based screens to study the effects of the microenvironment on cellular function.

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Showing Results 1 - 10 of 134
Scientific News
New 'Lab-on-a-Chip' Could Revolutionize Early Diagnosis of Cancer
Faster result times, reduced costs, minimal sample demands and better sensitivity of analysis.
How Fluid Flow Influences Neuron Growth
A University of Texas at Arlington team exploring how neuron growth can be controlled in the lab and, possibly, in the human body has published a new paper in Nature Scientific Reports on how fluid flow could play a significant role.
‘Tissue Chip’ to Screen Neurological Toxins
UW-Madison team are developing a faster, more affordable way to screen for neural toxins.
Modular Components Make Building 3-D “Labs-on-a-Chip” a Snap
New building blocks take microfluidics from flat to 3-D quickly and easily.
NIH Funds Next Phase of Tissue Chip for Drug Screening Program
Scientists will integrate chips mimicking human organ functions into full body system to evaluate drugs.
Airway Muscle-On-A-Chip Mimics Asthma
Tissue-level model of human airway musculature could pave way for patient-specific asthma treatments.
New Chip Promising For Tumor-Targeting Research
The new system, called a tumor-microenvironment-on-chip device, will allow researchers to study the complex environment surrounding tumors and the barriers that prevent the targeted delivery of therapeutic agents.
Gene Expression Patterns in Pancreatic CTCs Revealed
Distinct patterns of gene expression in several groups of CTCs were identified, including significant differences from the primary tumor that may contribute to the ability to generate metastases and prove to be targets for improved treatment of the deadly tumor.
Blood Cleanser for Sepsis
Dialysis-like ‘biospleen’ quickly filters bacteria, fungi and toxins.
Stanford Engineers Aim to Connect the World with Ant-sized Radios
Costing just pennies to make, tiny radios-on-a-chip are designed to serve as controllers or sensors for the 'Internet of Things.'
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