|Label-free Identification of Microorganisms using a Contact-less Dielectric Microsensor|
Ertl, P., Richter, L., Reinthaler, A., Stepper, C., Mak, A., Kast, M., Heer, R. and Brückl, H.
Microfabricated biochips are developed to continuously monitor cell population dynamics in a non-invasive manner. In the presented work we describe the novel combination of contact-less dielectric microsensors and microfluidics to promote biofilm formation for quantitative cell analysis.
|Design, Manufacturing and Test of Disposable Microfluidic System for Blood-Plasma Separation|
M. Kersaudy-Kerhoas, F. Amalou, D. Kavanagh, S. Marson, U. M. Attia, P. Summersgill, T. Ryan and M.P.Y. Desmulliez
Prenatal diagnosis to determine the outcome of pregnancies and detect conditions that may affect future pregnancies has risen as a big issue in the broad public. Analysis of fetal genetic material extracted from maternal blood is a smart alternative to invasive prenatal testing.
|EasyBeacons™ - new Probes Ideal for Realtime PCR Detection of Methylation Status of Single CpG Duplets and SNPs|
K. Skadhauge, C. Nielsen & U.B. Christensen
The EasyBeacons™ presented here are based on the novel technology Intercalating Nucleic Acid, INA®, linked to a fluorophore and a quencher. INA® is composed of normal DNA nucleotides and Intercalating Pseudo Nucleotides (IPNs). The fact that the EasyBeacons™ are mostly composed of normal DNA nucleotides means that in many respects EasyBeacons™ behave like DNA based probes, allowing use of standard buffers, primers and enzymes and hence reduces the optimisation efforts.
|Novel Fluidics Microbead Trap/Flow Cell Enhances Speed/Sensitivity of Bead-Based Bioassays Up to 5-Fold|
RM Ozanich, CJ Bruckner-Lea, JW Grate, MG Warner, BP Dockendorff, KC Antolick, HC Edberg, LH Johnson, AN Easterday
Pacific Northwest National Laboratory (PNNL) has developed a micro/nano particle trap that allows surface-functionalized magnetic or non-magnetic particles to be trapped with subsequent perfusion of sample, reagents and wash solutions, yielding significant (up to 5-fold) improvements in assay speed and sensitivity, while significantly reducing sample matrix effects.
|Utilizing High Speed Photography to Optimize Low Volume Dispensing Conditions |
Mary Cornett, Mitch Gordon and Anca Rothe
In this study we use high-speed photography as a feedback mechanism for adjusting the Nanodrop instrument dispense settings to improve the positional dispense accuracy of low volume (nanoliter) drops. These same parameters can be investigated, with various fluid classes, to reduce deleterious effects on dispensing performance such as deflected streams, satellite formation, secondary pulses and drop deformation.
|Detection of Genetically Modified Organisms Using DNA Microarrays|
Jaroslava Ovesna, Katerina Demnerova and Lucie Vistejnova
With the increasing production of genetically modified organis ms (GMOs ), the quick detection syst em is required. Microarrays offer a suitable and time saving method. Our aim is to develop DNA microarrays for detection of GMOs.
|Gene Expression in Cold-stressed Barley as Detected by Microarray Analysis|
J. Ovesná, B. Svejkovská, L. Kuèera, M. Malý, M. Herbstová and L. Cattivelli
Goal of this work is to find genes in spring and winter barleys that are influenced by cold stress and to analyze their expression profiles during stress. A few of experiments reported influence of temperature on the leaves and crown nodes, that is why we focus our study also on crown nodes by microarray technology.
|A Novel Array- Based Assay for the Detection of Ig G-Mediated Food Intolerance|
Andrew Macdonald, Michael J. Walker, Michael S. Walker and Julie G. Reeve
We have developed a microarray based immunoassay to permit both greater food panel diversity and higher throughput testing.
The Genarrayt™ 200+ Foods IgG test comprises of glass slides onto which 16 microarrays of over 200 different foods have been printed. Each microarray includes standards for quantitation and positive and negative controls for quality control. Food IgGs are detected by a novel fluorescent dye labelled anti-human IgG conjugate and results are measured using a laser scanner.
|Usage of Low-Density Oligonucleotide Microarrays for Prognosis Prediction of Colorectal Cancer Patients|
Slabý O., Garajová I., Svoboda M., Fabian P., Svoboda M., Šmerdová T. and Vyzula R.
This study aimed to find individual up/down-regulated genes associated with progression and metastatic potential of colorectal cancers using low-density oligonucleotide microarrays spotted with genes known to be involved in process of metastasis development. We suppose that focusing on a particular biological pathway may be more useful than genome-wide screening for our purposes.