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Tuesday, September 02, 2014
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Usage of Low-Density Oligonucleotide Microarrays for Prognosis Prediction of Colorectal Cancer Patients
Slabý O., Garajová I., Svoboda M., Fabian P., Svoboda M., Šmerdová T. and Vyzula R.

This study aimed to find individual up/down-regulated genes associated with progression and metastatic potential of colorectal cancers using low-density oligonucleotide microarrays spotted with genes known to be involved in process of metastasis development. We suppose that focusing on a particular biological pathway may be more useful than genome-wide screening for our purposes.

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Diagnosis of Aortic Aneurysm from Gene Expression Profiling of Peripheral Blood
Catalin Barbacioru,Yulei Wang, Dov Shiffman, Olga Iakoubova, Sriram Balasubramanian, Julie Blake, John Elefteriades and Raymond Samaha

We report in this study that gene expression profiles of peripheral blood cells may allow early detection and diagnosis of aortic aneurysm. Gene expression profiles of peripheral blood samples collected from 58 individuals diagnosed with thoracic aortic aneurysm (cases) and 36 normal individuals (controls) were analyzed using the Applied Biosystems Expression Array Systems and Human Genome Survey Microarrays.

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Microarray Analysis Using Competitive Hybridization
J. Bishop, C. Wilson, A. M. Chagovetz and S. Blair

In this work we explore the effects of wild-type and single nucleotide polymorphism (SNP) target specie concentrations, temperature, and the time of hybridization on sensing specificity in two component systems. A finite element method is used to simulate the diffusion of DNA through a microfluidic chamber to the sensing surface of bound oligonucleotide probes where hybridization of DNA is modeled using the corresponding chemical reaction equation assuming low grafting density.

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T7 Amplification for Generating Single Stranded Target for Hybridization to Allele-Specific DNA Microarrays
Jesper Petersen, Lena Poulsen, Henrik Birgens and Martin Dufva

Amplified RNA (aRNA) synthesized by T7 linear amplification is frequently used for gene expression analysis on microarrays. Single stranded DNA (ssDNA) is however mostly used as target in microarray based genotyping assays, although there are several advantages with aRNA. Here, we compared the performance of aRNA and ssDNA as targets in a genotyping assay, comprising a DNA microarray of allele specific oligo probes specific for thalassemic mutations.

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Quality Monitoring on Human 14K Oligo Chips at the University of Calgary
Xiuling Wang, Stephen M. Robbins and Mayi Arcellana-Panlilio,

The production of microarrays needs to be carefully monitored and quality control tests done to track systematic effects and performance variations over time to maximize the possibility of getting high quality data.

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New Platforms and Systems for DNA Microarrays
B. Henze, B. Saal, D. Drutschmann, K. Wellesen and P. Schüßler

Operon has designed probes of different lengths to various positions in the Open Reading Frames (ORFs) and the results clearly show that 70mers offer the optimal combination of specificity and sensitivity.

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S&S® Serum Biomarker Chip Displays Specificity & Reproducibility for 120 Different Human Biomarker Profiles
Christopher C. Zarozinski, Damon W. Pawlak, Brett A. Stillman, Michael A. Harvey and Breck O. Parker

We introduce a unique tool for the determination of relative abundances for human serum biomarkers. Conceptually similar to DNA microarrays, the S&S® Serum Biomarker Chip (SBC) is a single capture antibody array that was developed for comparative analysis of serum samples in order to identify differences or similarities in protein expression profiles.

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SPR Imaging of Protein Microarrays for Detection of Antibody Binding
J.B. Beusink, G.A.J. Besselink and R.B.M. Schasfoort

In our study we have used the imaging SPR Instrument for Biomolecular Interaction Sensing (iSPR-IBIS) (IBIS Technologies BV, Hengelo, The Netherlands), for monitoring the binding of biomolecules to a protein array in a fully automated manner. A TopSpot instrument from BioFluidix GmbH (Freiburg, Germany) was used to spot the protein array.

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Fully Automatic Analysis of DNA Microarray Images
S.Mitterhuber, B.Petersch and O.Serrano

During the last years, the advent and rapid development of DNA microarray techniques has revolutionized genetic research in life sciences. We are presenting a new stand-alone software for fully automated analysis of microarray images. It employs a number of novel image processing algorithms for grid detection and spot segmentation which assure high calculation speed and accuracy.

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Showing Results 91 - 100 of 128
Scientific News
Novel Chip-based Platform Could Simplify Measurements of Single Molecules
A nanopore-gated optofluidic chip combines electrical and optical measurements of single molecules onto a single platform.
Ingested Nanoparticles May Damage Liver
Although nanoparticles in food, sunscreen and other everyday products have many benefits, researchers from Cornell are finding that at certain doses, the particles might cause human organ damage.
Single-Cell Analysis Holds Promise for Stem Cell and Cancer Research
UCSF researchers use microfluidic technology to probe human brain development.
Researchers Invent Nanotech Microchip to Diagnose Type-1 Diabetes
The cheap, portable, microchip-based test could speed up diagnosis and enable studies of how the disease develops.
Revealing the Role of “Precocious” Dendritic Cells in Inflammatory Response
Discovery makes it possible for researchers to explore how these “precocious” cells escalate the body’s immune response when the body is under attack.
New NIST Metamaterial Gives Light a One-Way Ticket
The device could someday play a role in optical information processing and in novel biosensing devices.
Automating Laboratory-On-A-Chip To Cut Healthcare Costs
Computer programming language automates “laboratory-on-a-chip” technologies and has potential to improve and reduce cost of healthcare.
Chemical Sensor on a Chip
Using miniaturized laser technology, a tiny sensor has been built at the Vienna University of Technology which can test the chemical composition of liquids.
Miniature Gas Chromatograph Could Aid Early Crop Disease Detection
About the size of a 9-volt battery, the technology’s portability could provide farmers with a tool to quickly evaluate the health of their crops.
Ultra-Sensitive Nano-Chip for Early Cancer Detection
Device is able to detect very low concentrations of protein cancer markers, enabling diagnoses of the disease in its earliest stages.
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