|A Sandwich-Injection Method for Microchip Electrophoresis|
Gang Li, Gui-Sheng Zhuang, Hai-Bin Zhu, Hui-Qing Jin and Jian-Long Zhao
We present a sandwich-injection method for controlling discrete sample injection in µ-CE. This method involves four accessory arm channels in which symmetrical potentials are loaded to form a unique parallel electric field distribution to confine the spreading of sample plugs and prevent sample leakage. The virtues of the novel injection technique were demonstrated with numerical models and experiments.
|Large-scale Microarray Analysis of Protein and mRNA Level Changes in HL-60 Cells|
Pavel Simara, Irena Koutna, Stanislav Stejskal, Martina Peterkova, Petr Krontorad, Zdenek Rucka
In this study we compare methods for large-scale microarray analysis of protein and RNA level changes in HL-60 cells, responding to differentiation stimuli. Using microarrays we have found, that level of several proteins was either up- or down-regulated after cell differentiation. In some cases there was significant correlation with appropriate genes.
|Preliminary Report: The Geriatric Propamed Study: Prospective pharmacopgenetics in geriatrics|
Dr LS Griffith, Dr L Chialda and Dr A Pahl
In a worldwide first proscpective phamacogenetic study preliminary results show reduction of adverse drug reactions and hospitilisation stays for geriatric patients after pharmacogenetic testing and medication interaction analysis to fit the medicine to the patient.
|Microfluidic Platform for Point-of-Care Blood Typing|
B. Scheufele, R. Gronmaier, J. Claussen, S. Haeberle, J. Kohnle, J. Ducree, R. Zengerle, H. Northoff and F.K. Gehring
We have developed a microfluidic platform for mass sensitive analysis of whole blood that allows fast and reliable Point-of-Care blood typing of the AB0 system requiring small sample volumes. Potential application areas of the microfluidic platform include emergency medical aid, clinics and doctor's surgeries.
|Development of a Lab-on-a-Chip for the Characterization of Human Cells |
Richter, L., Stepper, C., Mak, A., Brückl, H. and Ertl, P.
Cell chips are developed to continuously monitor mammalian cell population dynamics in a non-invasive manner. In the presented work we describe the design, fabrication and characterization of a lab-on-a-chip for quantitative cell analysis.
|Label-free Identification of Microorganisms using a Contact-less Dielectric Microsensor|
Ertl, P., Richter, L., Reinthaler, A., Stepper, C., Mak, A., Kast, M., Heer, R. and Brückl, H.
Microfabricated biochips are developed to continuously monitor cell population dynamics in a non-invasive manner. In the presented work we describe the novel combination of contact-less dielectric microsensors and microfluidics to promote biofilm formation for quantitative cell analysis.
|Design, Manufacturing and Test of Disposable Microfluidic System for Blood-Plasma Separation|
M. Kersaudy-Kerhoas, F. Amalou, D. Kavanagh, S. Marson, U. M. Attia, P. Summersgill, T. Ryan and M.P.Y. Desmulliez
Prenatal diagnosis to determine the outcome of pregnancies and detect conditions that may affect future pregnancies has risen as a big issue in the broad public. Analysis of fetal genetic material extracted from maternal blood is a smart alternative to invasive prenatal testing.
|EasyBeacons™ - new Probes Ideal for Realtime PCR Detection of Methylation Status of Single CpG Duplets and SNPs|
K. Skadhauge, C. Nielsen & U.B. Christensen
The EasyBeacons™ presented here are based on the novel technology Intercalating Nucleic Acid, INA®, linked to a fluorophore and a quencher. INA® is composed of normal DNA nucleotides and Intercalating Pseudo Nucleotides (IPNs). The fact that the EasyBeacons™ are mostly composed of normal DNA nucleotides means that in many respects EasyBeacons™ behave like DNA based probes, allowing use of standard buffers, primers and enzymes and hence reduces the optimisation efforts.
|Novel Fluidics Microbead Trap/Flow Cell Enhances Speed/Sensitivity of Bead-Based Bioassays Up to 5-Fold|
RM Ozanich, CJ Bruckner-Lea, JW Grate, MG Warner, BP Dockendorff, KC Antolick, HC Edberg, LH Johnson, AN Easterday
Pacific Northwest National Laboratory (PNNL) has developed a micro/nano particle trap that allows surface-functionalized magnetic or non-magnetic particles to be trapped with subsequent perfusion of sample, reagents and wash solutions, yielding significant (up to 5-fold) improvements in assay speed and sensitivity, while significantly reducing sample matrix effects.