Posters

The aim of this project was to develop high throughput screening methods using an IMSTAR Pathfinder™ High-content imaging platform to support the analyses of polymeric materials synthesised using a parallel approach. This was achieved by developing arrays of polymeric materials.

Application of microarrays during process development helps to gain insight in biological processes involved in product formation, increasing process understanding.

In this study we have used high-resolution genome-wide DNA copy number screening by array-based comparative genomic hybridization (array CGH)2-3 to identify the underlying genetic cause of this malformation syndrome. Through this screening we identified overlapping microdeletions on chromosome 8 in two CHARGE patients.

A competitive study analysing the background fluorescence and the detectable concentration range of a chosen antigen is presented. An Affymetrix Spotting roboter 417 arrayer was used for protein dotting. The analysis is based on fluorescence scanning experiments with an Affymetrix 428 array scanner at a gain of 60. Data analysis was made with ImaGene Premium 5.5.3 by Biodiscovery Inc.

We have developed a workflow for optimisation of array preparation and for processing protocols. Only 4 independent model experiments and solvent, immobilisation and blocking conditions. Optimal conditions or high quality microarray experiments were determined and used for array production and slide processing at the FGCZ.

A microarray test for in vitro diagnostic use that contains 27 SNPs from 8 different genes with a proven role in the metabolism of major drugs and that may be processed using regular laboratory instrumentation and a range of different scanners.

Results suggest that random priming is the optimal method for expression analysis of long genes. This method of RT may be appropriate for alternatively spliced genes and for genes without unique probes in the 3’-region.

The Universal Linkage System (ULS™) is a platinum-based technology that allows direct labeling of proteins and nucleic acids with a large variety of labels and haptens, including many fluorophores, biotin, and dinitrophenol.

We present here an online system to handle Affymetrix sample submission and tracking, job management and lab processing. Many current microarray database solutions tend to focus on post sample-processing analysis. GenomeLIMS allows recording of all sample processing steps, there are no expression data analysis functions.