|SPR Imaging of Protein Microarrays for Detection of Antibody Binding|
J.B. Beusink, G.A.J. Besselink and R.B.M. Schasfoort
In our study we have used the imaging SPR Instrument for Biomolecular Interaction Sensing (iSPR-IBIS) (IBIS Technologies BV, Hengelo, The Netherlands), for monitoring the binding of biomolecules to a protein array in a fully automated manner. A TopSpot instrument from BioFluidix GmbH (Freiburg, Germany) was used to spot the protein array.
|Fully Automatic Analysis of DNA Microarray Images|
S.Mitterhuber, B.Petersch and O.Serrano
During the last years, the advent and rapid development of DNA microarray techniques has revolutionized genetic research in life sciences. We are presenting a new stand-alone software for fully automated analysis of microarray images. It employs a number of novel image processing algorithms for grid detection and spot segmentation which assure high calculation speed and accuracy.
|Design and Functional Analysis of ssDNA Directed Assembly in Protein Array|
Ng Jin Kiata, Parayil Kumaran Ajikumara, Tang Yew Chunga, Lee Jim Yanga, Gregory Stephanopoulosa and Too Heng-Phona
The present poster discusses the fabrication and evaluation of a new platform called “Spatially addressable protein array” (SAPA). By exploring the specificity of DNA hybridization, ssDNA-antibody conjugates would capture the antigen from complex biological samples in milieu and spatially addressed to specific location on the oligo array for detection.
|Automated Multi-Layered Multiplex Assay Production with Inkjet Technology|
Susan Seaton, Duncan Hall and Howard Manning
This work reports on the production and exact reproducible nature of the Aj120 Inkjet Microarray Spotter when printing multiple droplet microarrays. This has lead to successful collaborations between Arrayjet and a number of scientific groups furthering the use of this novel method of non-contact printing within the microarray field, a few of which are highlighted here.
|Prediction of Protein-to-Protein Interactions|
C.Gilissen, P.Groot, P.Lucas, J.Veltman, A.GeurtsvanKessel and M.Egmont-Petersen
We use local extrema in microarray time series data as the basis for discretisation. By comparing discretised gene expressions using similarity functions, we discover putative protein-to-protein interactions. We validate the results by use of public true positive and true negative databases for protein-to-protein interactions and demonstrate the high predictiveness of these local extrema as a time series feature.
|Constructing Directed Metabolic Networks from Microarray Data|
J. M. Easton, T. N. Arvanitis, A. Peet and M. Viant
Although it has been several years since metabolic networks became a commonly used analysis technique in bioinformatics, the question of how best to construct them from experimental data is still not satisfactorily resolved. Here we present a method for the construction of directed metabolic networks from microarray datasets using an enhanced version of the KEGG LIGAND database.
|Biosensors using Surface Plasmon Resonance Dynamic Imaging|
J. Hottin, J. Spadavecchia, M. Canva, E. Maillart, M. Anger-Leroy and P. Kerourédan
We study the optical response of a SPRi detection system, characterizing temperature, stringency and time dependence of molecular interactions for the development of a DNA biochip. We are adapting this system and improve its robustness developing applications, as a 900 spots microarray identifying 65 mutations of the cystic fibrosis, for medical uses and industrial commercialization.
|A Novel Array-Based Assay for the Detection of IgG-Mediated Food Intolerance |
Andrew Macdonald, Michael J. Walker, Michael S. Walker and Julie G. Reeve
The Genarrayt 200+ Foods IgG test comprises of glass slides onto which 16 microarrays of over 200 different foods have been printed. Each microarray includes standards for quantitation and positive and negative controls for quality control. Food IgGs are detected by a novel fluorescent dye labelled anti-human IgG conjugate and results are measured using a laser scanner. Fluorescence intensity is directly proportional to antibody activity in the sample.
|Clinical Validation of a Protein Microarry Assay for Diagnostics of Autoimmune Disease|
S. Judice, D. Pawlak, H Appelhans, A. Frisse, M. Harvey and B. Stillman
We have developed an autoantigen microarray that will allow laboratories to measure the presence of autoantibobies associated with different collagenois and vasculitis-related autoimmune diseases for a single serum sample.