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Wednesday, October 01, 2014
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S&S® Serum Biomarker Chip Displays Specificity & Reproducibility for 120 Different Human Biomarker Profiles
Christopher C. Zarozinski, Damon W. Pawlak, Brett A. Stillman, Michael A. Harvey and Breck O. Parker

We introduce a unique tool for the determination of relative abundances for human serum biomarkers. Conceptually similar to DNA microarrays, the S&S® Serum Biomarker Chip (SBC) is a single capture antibody array that was developed for comparative analysis of serum samples in order to identify differences or similarities in protein expression profiles.

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SPR Imaging of Protein Microarrays for Detection of Antibody Binding
J.B. Beusink, G.A.J. Besselink and R.B.M. Schasfoort

In our study we have used the imaging SPR Instrument for Biomolecular Interaction Sensing (iSPR-IBIS) (IBIS Technologies BV, Hengelo, The Netherlands), for monitoring the binding of biomolecules to a protein array in a fully automated manner. A TopSpot instrument from BioFluidix GmbH (Freiburg, Germany) was used to spot the protein array.

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Fully Automatic Analysis of DNA Microarray Images
S.Mitterhuber, B.Petersch and O.Serrano

During the last years, the advent and rapid development of DNA microarray techniques has revolutionized genetic research in life sciences. We are presenting a new stand-alone software for fully automated analysis of microarray images. It employs a number of novel image processing algorithms for grid detection and spot segmentation which assure high calculation speed and accuracy.

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Design and Functional Analysis of ssDNA Directed Assembly in Protein Array
Ng Jin Kiata, Parayil Kumaran Ajikumara, Tang Yew Chunga, Lee Jim Yanga, Gregory Stephanopoulosa and Too Heng-Phona

The present poster discusses the fabrication and evaluation of a new platform called “Spatially addressable protein array” (SAPA). By exploring the specificity of DNA hybridization, ssDNA-antibody conjugates would capture the antigen from complex biological samples in milieu and spatially addressed to specific location on the oligo array for detection.

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Automated Multi-Layered Multiplex Assay Production with Inkjet Technology
Susan Seaton, Duncan Hall and Howard Manning

This work reports on the production and exact reproducible nature of the Aj120 Inkjet Microarray Spotter when printing multiple droplet microarrays. This has lead to successful collaborations between Arrayjet and a number of scientific groups furthering the use of this novel method of non-contact printing within the microarray field, a few of which are highlighted here.

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Prediction of Protein-to-Protein Interactions
C.Gilissen, P.Groot, P.Lucas, J.Veltman, A.GeurtsvanKessel and M.Egmont-Petersen

We use local extrema in microarray time series data as the basis for discretisation. By comparing discretised gene expressions using similarity functions, we discover putative protein-to-protein interactions. We validate the results by use of public true positive and true negative databases for protein-to-protein interactions and demonstrate the high predictiveness of these local extrema as a time series feature.

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Constructing Directed Metabolic Networks from Microarray Data
J. M. Easton, T. N. Arvanitis, A. Peet and M. Viant

Although it has been several years since metabolic networks became a commonly used analysis technique in bioinformatics, the question of how best to construct them from experimental data is still not satisfactorily resolved. Here we present a method for the construction of directed metabolic networks from microarray datasets using an enhanced version of the KEGG LIGAND database.

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Biosensors using Surface Plasmon Resonance Dynamic Imaging
J. Hottin, J. Spadavecchia, M. Canva, E. Maillart, M. Anger-Leroy and P. Kerourédan

We study the optical response of a SPRi detection system, characterizing temperature, stringency and time dependence of molecular interactions for the development of a DNA biochip. We are adapting this system and improve its robustness developing applications, as a 900 spots microarray identifying 65 mutations of the cystic fibrosis, for medical uses and industrial commercialization.

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A Novel Array-Based Assay for the Detection of IgG-Mediated Food Intolerance
Andrew Macdonald, Michael J. Walker, Michael S. Walker and Julie G. Reeve

The Genarrayt 200+ Foods IgG test comprises of glass slides onto which 16 microarrays of over 200 different foods have been printed. Each microarray includes standards for quantitation and positive and negative controls for quality control. Food IgGs are detected by a novel fluorescent dye labelled anti-human IgG conjugate and results are measured using a laser scanner. Fluorescence intensity is directly proportional to antibody activity in the sample.

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Showing Results 101 - 110 of 132
Scientific News
UW-Madison Team Developing ‘Tissue Chip’ to Screen Neurological Toxins
A faster, more affordable way to screen for neural toxins.
Modular Components Make Building 3-D “Labs-on-a-Chip” a Snap
New building blocks take microfluidics from flat to 3-D quickly and easily.
NIH Funds Next Phase of Tissue Chip for Drug Screening Program
Scientists will integrate chips mimicking human organ functions into full body system to evaluate drugs.
Airway Muscle-On-A-Chip Mimics Asthma
Tissue-level model of human airway musculature could pave way for patient-specific asthma treatments.
New Chip Promising For Tumor-Targeting Research
The new system, called a tumor-microenvironment-on-chip device, will allow researchers to study the complex environment surrounding tumors and the barriers that prevent the targeted delivery of therapeutic agents.
Gene Expression Patterns in Pancreatic CTCs Revealed
Distinct patterns of gene expression in several groups of CTCs were identified, including significant differences from the primary tumor that may contribute to the ability to generate metastases and prove to be targets for improved treatment of the deadly tumor.
Blood Cleanser for Sepsis
Dialysis-like ‘biospleen’ quickly filters bacteria, fungi and toxins.
Stanford Engineers Aim to Connect the World with Ant-sized Radios
Costing just pennies to make, tiny radios-on-a-chip are designed to serve as controllers or sensors for the 'Internet of Things.'
Lab on a Breathing Chip
Human nasal epithelial cells, cultured on a microchip, react to air pollutants just like they would in the upper airway.
Micropumps for Lab-on-a-Chip Disease Diagnosis
Reliable, inexpensive, programmable pumps could help make the diagnosis of many global life-threatening diseases easy and affordable.
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