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Tuesday, September 02, 2014
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Photochemical Modification of Cyclic Olefin Copolymer Microfluidic Chips for Biomolecule Microarrays and Surface Property Patterning
Qiaosheng Pu, Olufemi Oyesanya, Bowlin Thompson and Julio C. Alvarez

Cyclic olefin copolymer(COC) was used for fabrication of bio-chips. The surface of COC, either outside or inside the micro-channels was modified/patterned with UV initiated surface grafting polymerization of acrylic monomers for obtaining different hydrophilicity, surface charge and reactive groups. DNA, avidin and biotin arrays were made through carboxylic patterns.

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Fast Development Strategy: One-Week-to-Chip
K.S.Dresse, G.Munchow and M.Ritzi

Mass manufacturing techniques like injection molding and lamination processes that allow the production of final disposable products at reasonable costs. What is needed is a process that allows fast tests of concepts and for the validation of the final chip design. As such tests pick up more and more speed the more you can rely on already established elements.

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Turning Valves for Lab-on-a-Chip Applications Enable Directional Flow and Portion Out Pre-Defined Volumes
R. Gransee, D. Dadic, F. Doffing, E. Schaeffer and K. S. Drese.

In this study the design and the realization of low-cost valves suitable for the integration into disposable polymer chips is presented. In contrast to commonly used sandwich-setups a plain design consisting of a simply structured polymer chip and a cover membrane is used.

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A Valve-Controlled Microfluidic Sysytem for Rapid Two-Dimensional Electrophoresis
Yiqi Luo, Bo Huang, Michael P. Bokoch, Richard N. Zare.

Two-dimensional electrophoresis of proteins is achieved in 500 seconds in a microfluidic system fabricated from PDMS, in which the first dimension of micellar electrokinetic chromatography and the second dimension of capillary sieving electrophoresis are coupled. By installing valves at the intersection of two dimensions, the separations are performed sequentially without interference.

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High Throughput Screening of Biocompatible Polymers
Guilhem A. Tourniaire, Jerome Sallette and Mark Bradley

The aim of this project was to develop high throughput screening methods using an IMSTAR Pathfinder™ High-content imaging platform to support the analyses of polymeric materials synthesised using a parallel approach. This was achieved by developing arrays of polymeric materials.

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Application of Microarrays in Process Analytical Technologies A First Step Towards Real Time Product Release
Streefland M, van de Waterbeemd B, vander Pol LA, Beuvery EC, Martens DE,Tramper J

Application of microarrays during process development helps to gain insight in biological processes involved in product formation, increasing process understanding.

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Gene Identification Through Array CGH: The CHARGE Syndrome
Lisenka Vissers, Conny van Ravenswaaij, Ronald Admiraal, Jane Hurst, Bert de Vries, Irene Janssen, Walter van der Vliet, Erik Huys, Pieter de Jong, Ben Hamel, Eric Schoenmakers, Han Brunner, Joris Veltman and Ad Geurts van Kessel.

In this study we have used high-resolution genome-wide DNA copy number screening by array-based comparative genomic hybridization (array CGH)2-3 to identify the underlying genetic cause of this malformation syndrome. Through this screening we identified overlapping microdeletions on chromosome 8 in two CHARGE patients.

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New 3D Black Substrate for Protein Microarrays with Improved Dynamic Range
Markus Hollas, Eric Jallerat, Karl Pflanz, Inka Praulich, Johanna Walter

A competitive study analysing the background fluorescence and the detectable concentration range of a chosen antigen is presented. An Affymetrix Spotting roboter 417 arrayer was used for protein dotting. The analysis is based on fluorescence scanning experiments with an Affymetrix 428 array scanner at a gain of 60. Data analysis was made with ImaGene Premium 5.5.3 by Biodiscovery Inc.

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Optimisation Workflow for the Production and Processing of High Quality Microarrays Applications in DNA, Peptide, antibody, and Carbohydrate Microarraying
Jens Sobek, Catharine Aquino and Ralph Schlapbach

We have developed a workflow for optimisation of array preparation and for processing protocols. Only 4 independent model experiments and solvent, immobilisation and blocking conditions. Optimal conditions or high quality microarray experiments were determined and used for array production and slide processing at the FGCZ.

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Showing Results 111 - 120 of 128
Scientific News
Novel Chip-based Platform Could Simplify Measurements of Single Molecules
A nanopore-gated optofluidic chip combines electrical and optical measurements of single molecules onto a single platform.
Ingested Nanoparticles May Damage Liver
Although nanoparticles in food, sunscreen and other everyday products have many benefits, researchers from Cornell are finding that at certain doses, the particles might cause human organ damage.
Single-Cell Analysis Holds Promise for Stem Cell and Cancer Research
UCSF researchers use microfluidic technology to probe human brain development.
Researchers Invent Nanotech Microchip to Diagnose Type-1 Diabetes
The cheap, portable, microchip-based test could speed up diagnosis and enable studies of how the disease develops.
Revealing the Role of “Precocious” Dendritic Cells in Inflammatory Response
Discovery makes it possible for researchers to explore how these “precocious” cells escalate the body’s immune response when the body is under attack.
New NIST Metamaterial Gives Light a One-Way Ticket
The device could someday play a role in optical information processing and in novel biosensing devices.
Automating Laboratory-On-A-Chip To Cut Healthcare Costs
Computer programming language automates “laboratory-on-a-chip” technologies and has potential to improve and reduce cost of healthcare.
Chemical Sensor on a Chip
Using miniaturized laser technology, a tiny sensor has been built at the Vienna University of Technology which can test the chemical composition of liquids.
Miniature Gas Chromatograph Could Aid Early Crop Disease Detection
About the size of a 9-volt battery, the technology’s portability could provide farmers with a tool to quickly evaluate the health of their crops.
Ultra-Sensitive Nano-Chip for Early Cancer Detection
Device is able to detect very low concentrations of protein cancer markers, enabling diagnoses of the disease in its earliest stages.
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