|Simplifying the Flow of Drug Discovery Data|
Dr. Jonathan M.R. Davies
Regardless of research disciplines, scientists need to easily reach the information pertinent to their research. Ideally this data access is easy. Researchers also need the ability to ‘move the data around’ to gain a better view or different perspective. This data manipulation needs to be straightforward. Incorporating the varying views and information required by different scientific disciplines is a considerable challenge.
|DNA Microarrays for Microbiological Diagnosis in Stem Cell Cultures|
F. Cobo, A. Nieto, P. Catalina, JL. Cortés, A. Barroso, C. Cabrera, R. Montes, A. Barnie and A. Concha
In stem cell cultures there is the possibility of infectious disease transmission to the recipients. Any microbial contamination of the donor`s biological products or introduced during the manufacturing process can potentially present a serious hazard to the recipients. The majority of potential contaminants are mycoplasma and other bacterias, yeasts and fungi. Moreover, viral and prion contamination of cell cultures and “feeder” cells is also a common risk.
|New Methods for Rapid Isothermal Amplification and Detection of Short DNA Sequences |
Goal: • Rapid, sensitive, specific, low tech, portable DNA diagnostic device
• Detection of clinical pathogens: SARS pathogen, Streptococcus pneumoniae, HSV I & II and biothreat agents: Bacillus anthracis, Brucella species
• Detection of single nucleotide polymorphisms (SNPs)
• Multiplexed detection format
|Biomathematical Information Compression and Signal Extraction for Gene Expression Microarray Data Analysis|
Sofiane Lariani, Elena Comelli, Patrick Descombes and Martin Grigorov
Gene expression Microarray data covers both signal (active key genes) and noise. A tentative to recognize any genes expression pattern without clearing the data is like recognizing faces and details on a jammed TV screen. Noise in this context can be related to fluctuation due to the experimental condition, to outliers, and also genes presenting no modulation between groups. In this work we present a biostatisticalmethod for noise filtering and statistical significance assessment.
|Photochemical Modification of Cyclic Olefin Copolymer Microfluidic Chips for Biomolecule Microarrays and Surface Property Patterning|
Qiaosheng Pu, Olufemi Oyesanya, Bowlin Thompson and Julio C. Alvarez
Cyclic olefin copolymer(COC) was used for fabrication of bio-chips. The surface of COC, either outside or inside the micro-channels was modified/patterned with UV initiated surface grafting polymerization of acrylic monomers for obtaining different hydrophilicity, surface charge and reactive groups. DNA, avidin and biotin arrays were made through carboxylic patterns.
|Fast Development Strategy: One-Week-to-Chip|
K.S.Dresse, G.Munchow and M.Ritzi
Mass manufacturing techniques like injection molding and lamination processes that allow the production of final disposable products at reasonable costs. What is needed is a process that allows fast tests of concepts and for the validation of the final chip design. As such tests pick up more and more speed the more you can rely on already established elements.
|Turning Valves for Lab-on-a-Chip Applications Enable Directional Flow and Portion Out Pre-Defined Volumes|
R. Gransee, D. Dadic, F. Doffing, E. Schaeffer and K. S. Drese.
In this study the design and the realization of low-cost valves suitable for the integration into disposable polymer chips is presented. In contrast to commonly used sandwich-setups a plain design consisting of a simply structured polymer chip and a cover membrane is used.
|A Valve-Controlled Microfluidic Sysytem for Rapid Two-Dimensional Electrophoresis|
Yiqi Luo, Bo Huang, Michael P. Bokoch, Richard N. Zare.
Two-dimensional electrophoresis of proteins is achieved in 500 seconds in a microfluidic system fabricated from PDMS, in which the first dimension of micellar electrokinetic chromatography and the second dimension of capillary sieving electrophoresis are coupled. By installing valves at the intersection of two dimensions, the separations are performed sequentially without interference.
|High Throughput Screening of Biocompatible Polymers|
Guilhem A. Tourniaire, Jerome Sallette and Mark Bradley
The aim of this project was to develop high throughput screening methods using an IMSTAR Pathfinder™ High-content imaging platform to support the analyses of polymeric materials synthesised using a parallel approach. This was achieved by developing arrays of polymeric materials.