|Exploring Reverse Transcription for DNA MicroArrays|
Miroslava Cuperlovic-Culf, Adrian S. Culf, Dominique Richard, Mark LaFlamme, Daniel Leger and Rodney J. Ouellette
Results suggest that random priming is the optimal method for expression analysis of long genes. This method of RT may be appropriate for alternatively spliced genes and for genes without unique probes in the 3’-region.
|Protein Expression Profiling with High-Content Antibody Arrays using Universal Linkage System ULS™ Protein Labeling|
Frank Opdam, Saskia Schoenmakers, Marie Lacombe, Sandra van der Locht, Alain van Gool and Paul van der Hoeven
The Universal Linkage System (ULS™) is a platinum-based technology that allows direct labeling of proteins and nucleic acids with a large variety of labels and haptens, including many fluorophores, biotin, and dinitrophenol.
|GenomeLIMs: Affymetrix Expression module for GeneChip Expression Array Sample Submission and Tracking|
James Hadfield, Rob Davey, Jenn Conn
We present here an online system to handle Affymetrix sample submission and tracking, job management and lab processing. Many current microarray database solutions tend to focus on post sample-processing analysis. GenomeLIMS allows recording of all sample processing steps, there are no expression data analysis functions.
|Carbon Nanotubes by Microwave Plasma Enhanced Chemical Vapor Deposition|
M. Maschmann, A. Goyal, Z. Iqbal, T.S. Fisher, R. Gat
MICROWAVE Plasma generates radical density characteristic of 2000C at much lower effective temperature and can be highly ionized (Low pressure ECR mode) or highly neutral (high pressure mode).
|rptDB; a prototype Affymetrix .rpt QC tool|
James Hadfield, Marielle Vigouroux
rptDB is a prototype database for the analysis of Affymetrix expression report (.rpt) files. Report files are widely used to QC raw data after hyb, scan and .cel file generation. Only %Present calls, Scale Factors and 3':5' ratios of housekeeping genes are generally used, but there are many other metrics that may also be of importance for QC analysis.
|Chip’N’Fish : Novel Chemical Proteomics Platform for Rapid Identification of Small Molecule Drug Candidates Against Disease-Specific Targets|
György Dormán, László Puskás, László Hackler, Liliana Z. Fehér, Cesare Spadoni , Zsolt Lörincz, Tamás Jankovics, László Ürge and Ferenc Darvas
Over-expression of genes and their protein products is often the best starting point for dissecting the pathways that are responsible for the development of specific diseases.The Chip'N'Fish approach allows identifying over-expressed proteins directly from cell extracts of disease origin using healthy counterparts as controls.
|High-Throughput Automation of a Dual Reporter Assay in Low Volume 384 & 1536 Well Plate Formats |
Aoife Gallagher, Brad Larson, Tracy Worzella, Michael Bjerke and Eric Matthews
The drive towards miniaturization within the pharmaceutical and biotechnology fields has created a need for liquid handling technologies that accurately deliver low volume reagents to high-density plates. This has also created a need for simple, fully scaleable assays in low volumes. Here we demonstrate the successful combination of both through the use of the Equator™ NS 808 - Eight Tip Pipetting System and the dual color Chroma-Luc™ technology.
|Miniaturisation of Liquid Handling Procedures for High Throughput Sequencing |
Joe Graham, Sheila Fisher, Tracey Honan, Susan Faro and Aoife Gallagher
Deerac Fluidics Equator™ products are designed to fit a wide range of applications requiring low volume liquid handling. The products have recently been installed as part of the high throughput sequencing production process at the Broad Institute in Boston, MA, USA. This poster will describe the current process employed by Broad Institute, highlighting where the Equator™ has played a vital role in performance enhancement and cost reduction, enabling the Broad Institute to retain its position as
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