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Monday, September 22, 2014
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DNA Microarrays for Microbiological Diagnosis in Stem Cell Cultures
F. Cobo, A. Nieto, P. Catalina, JL. Cortés, A. Barroso, C. Cabrera, R. Montes, A. Barnie and A. Concha

In stem cell cultures there is the possibility of infectious disease transmission to the recipients. Any microbial contamination of the donor`s biological products or introduced during the manufacturing process can potentially present a serious hazard to the recipients. The majority of potential contaminants are mycoplasma and other bacterias, yeasts and fungi. Moreover, viral and prion contamination of cell cultures and “feeder” cells is also a common risk.

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New Methods for Rapid Isothermal Amplification and Detection of Short DNA Sequences

Goal: • Rapid, sensitive, specific, low tech, portable DNA diagnostic device • Detection of clinical pathogens: SARS pathogen, Streptococcus pneumoniae, HSV I & II and biothreat agents: Bacillus anthracis, Brucella species • Detection of single nucleotide polymorphisms (SNPs) • Multiplexed detection format

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Biomathematical Information Compression and Signal Extraction for Gene Expression Microarray Data Analysis
Sofiane Lariani, Elena Comelli, Patrick Descombes and Martin Grigorov

Gene expression Microarray data covers both signal (active key genes) and noise. A tentative to recognize any genes expression pattern without clearing the data is like recognizing faces and details on a jammed TV screen. Noise in this context can be related to fluctuation due to the experimental condition, to outliers, and also genes presenting no modulation between groups. In this work we present a biostatisticalmethod for noise filtering and statistical significance assessment.

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Photochemical Modification of Cyclic Olefin Copolymer Microfluidic Chips for Biomolecule Microarrays and Surface Property Patterning
Qiaosheng Pu, Olufemi Oyesanya, Bowlin Thompson and Julio C. Alvarez

Cyclic olefin copolymer(COC) was used for fabrication of bio-chips. The surface of COC, either outside or inside the micro-channels was modified/patterned with UV initiated surface grafting polymerization of acrylic monomers for obtaining different hydrophilicity, surface charge and reactive groups. DNA, avidin and biotin arrays were made through carboxylic patterns.

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Fast Development Strategy: One-Week-to-Chip
K.S.Dresse, G.Munchow and M.Ritzi

Mass manufacturing techniques like injection molding and lamination processes that allow the production of final disposable products at reasonable costs. What is needed is a process that allows fast tests of concepts and for the validation of the final chip design. As such tests pick up more and more speed the more you can rely on already established elements.

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Turning Valves for Lab-on-a-Chip Applications Enable Directional Flow and Portion Out Pre-Defined Volumes
R. Gransee, D. Dadic, F. Doffing, E. Schaeffer and K. S. Drese.

In this study the design and the realization of low-cost valves suitable for the integration into disposable polymer chips is presented. In contrast to commonly used sandwich-setups a plain design consisting of a simply structured polymer chip and a cover membrane is used.

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A Valve-Controlled Microfluidic Sysytem for Rapid Two-Dimensional Electrophoresis
Yiqi Luo, Bo Huang, Michael P. Bokoch, Richard N. Zare.

Two-dimensional electrophoresis of proteins is achieved in 500 seconds in a microfluidic system fabricated from PDMS, in which the first dimension of micellar electrokinetic chromatography and the second dimension of capillary sieving electrophoresis are coupled. By installing valves at the intersection of two dimensions, the separations are performed sequentially without interference.

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High Throughput Screening of Biocompatible Polymers
Guilhem A. Tourniaire, Jerome Sallette and Mark Bradley

The aim of this project was to develop high throughput screening methods using an IMSTAR Pathfinder™ High-content imaging platform to support the analyses of polymeric materials synthesised using a parallel approach. This was achieved by developing arrays of polymeric materials.

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Application of Microarrays in Process Analytical Technologies A First Step Towards Real Time Product Release
Streefland M, van de Waterbeemd B, vander Pol LA, Beuvery EC, Martens DE,Tramper J

Application of microarrays during process development helps to gain insight in biological processes involved in product formation, increasing process understanding.

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Showing Results 111 - 120 of 131
Scientific News
Blood Cleanser for Sepsis
Dialysis-like ‘biospleen’ quickly filters bacteria, fungi and toxins.
Stanford Engineers Aim to Connect the World with Ant-sized Radios
Costing just pennies to make, tiny radios-on-a-chip are designed to serve as controllers or sensors for the 'Internet of Things.'
Lab on a Breathing Chip
Human nasal epithelial cells, cultured on a microchip, react to air pollutants just like they would in the upper airway.
Micropumps for Lab-on-a-Chip Disease Diagnosis
Reliable, inexpensive, programmable pumps could help make the diagnosis of many global life-threatening diseases easy and affordable.
Novel Chip-based Platform Could Simplify Measurements of Single Molecules
A nanopore-gated optofluidic chip combines electrical and optical measurements of single molecules onto a single platform.
Ingested Nanoparticles May Damage Liver
Although nanoparticles in food, sunscreen and other everyday products have many benefits, researchers from Cornell are finding that at certain doses, the particles might cause human organ damage.
Single-Cell Analysis Holds Promise for Stem Cell and Cancer Research
UCSF researchers use microfluidic technology to probe human brain development.
Researchers Invent Nanotech Microchip to Diagnose Type-1 Diabetes
The cheap, portable, microchip-based test could speed up diagnosis and enable studies of how the disease develops.
Revealing the Role of “Precocious” Dendritic Cells in Inflammatory Response
Discovery makes it possible for researchers to explore how these “precocious” cells escalate the body’s immune response when the body is under attack.
New NIST Metamaterial Gives Light a One-Way Ticket
The device could someday play a role in optical information processing and in novel biosensing devices.
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