|Biomathematical Information Compression and Signal Extraction for Gene Expression Microarray Data Analysis|
Sofiane Lariani, Elena Comelli, Patrick Descombes and Martin Grigorov
Gene expression Microarray data covers both signal (active key genes) and noise. A tentative to recognize any genes expression pattern without clearing the data is like recognizing faces and details on a jammed TV screen. Noise in this context can be related to fluctuation due to the experimental condition, to outliers, and also genes presenting no modulation between groups. In this work we present a biostatisticalmethod for noise filtering and statistical significance assessment.
|Photochemical Modification of Cyclic Olefin Copolymer Microfluidic Chips for Biomolecule Microarrays and Surface Property Patterning|
Qiaosheng Pu, Olufemi Oyesanya, Bowlin Thompson and Julio C. Alvarez
Cyclic olefin copolymer(COC) was used for fabrication of bio-chips. The surface of COC, either outside or inside the micro-channels was modified/patterned with UV initiated surface grafting polymerization of acrylic monomers for obtaining different hydrophilicity, surface charge and reactive groups. DNA, avidin and biotin arrays were made through carboxylic patterns.
|Fast Development Strategy: One-Week-to-Chip|
K.S.Dresse, G.Munchow and M.Ritzi
Mass manufacturing techniques like injection molding and lamination processes that allow the production of final disposable products at reasonable costs. What is needed is a process that allows fast tests of concepts and for the validation of the final chip design. As such tests pick up more and more speed the more you can rely on already established elements.
|Turning Valves for Lab-on-a-Chip Applications Enable Directional Flow and Portion Out Pre-Defined Volumes|
R. Gransee, D. Dadic, F. Doffing, E. Schaeffer and K. S. Drese.
In this study the design and the realization of low-cost valves suitable for the integration into disposable polymer chips is presented. In contrast to commonly used sandwich-setups a plain design consisting of a simply structured polymer chip and a cover membrane is used.
|A Valve-Controlled Microfluidic Sysytem for Rapid Two-Dimensional Electrophoresis|
Yiqi Luo, Bo Huang, Michael P. Bokoch, Richard N. Zare.
Two-dimensional electrophoresis of proteins is achieved in 500 seconds in a microfluidic system fabricated from PDMS, in which the first dimension of micellar electrokinetic chromatography and the second dimension of capillary sieving electrophoresis are coupled. By installing valves at the intersection of two dimensions, the separations are performed sequentially without interference.
|High Throughput Screening of Biocompatible Polymers|
Guilhem A. Tourniaire, Jerome Sallette and Mark Bradley
The aim of this project was to develop high throughput screening methods using an IMSTAR Pathfinder™ High-content imaging platform to support the analyses of polymeric materials synthesised using a parallel approach. This was achieved by developing arrays of polymeric materials.
|Application of Microarrays in Process Analytical Technologies A First Step Towards Real Time Product Release|
Streefland M, van de Waterbeemd B, vander Pol LA, Beuvery EC, Martens DE,Tramper J
Application of microarrays during process development helps to gain insight in biological processes involved in product formation, increasing process understanding.
|Gene Identification Through Array CGH: The CHARGE Syndrome|
Lisenka Vissers, Conny van Ravenswaaij, Ronald Admiraal, Jane Hurst, Bert de Vries, Irene Janssen, Walter van der Vliet, Erik Huys, Pieter de Jong, Ben Hamel, Eric Schoenmakers, Han Brunner, Joris Veltman and Ad Geurts van Kessel.
In this study we have used high-resolution genome-wide DNA copy number screening by array-based comparative genomic hybridization (array CGH)2-3 to identify the underlying genetic cause of this malformation syndrome. Through this screening we identified overlapping microdeletions on chromosome 8 in two CHARGE patients.
|New 3D Black Substrate for Protein Microarrays with Improved Dynamic Range|
Markus Hollas, Eric Jallerat, Karl Pflanz, Inka Praulich, Johanna Walter
A competitive study analysing the background fluorescence and the detectable concentration range of a chosen antigen is presented. An Affymetrix Spotting roboter 417 arrayer was used for protein dotting. The analysis is based on fluorescence scanning experiments with an Affymetrix 428 array scanner at a gain of 60. Data analysis was made with ImaGene Premium 5.5.3 by Biodiscovery Inc.