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Sunday, February 01, 2015
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Photochemical Modification of Cyclic Olefin Copolymer Microfluidic Chips for Biomolecule Microarrays and Surface Property Patterning
Qiaosheng Pu, Olufemi Oyesanya, Bowlin Thompson and Julio C. Alvarez

Cyclic olefin copolymer(COC) was used for fabrication of bio-chips. The surface of COC, either outside or inside the micro-channels was modified/patterned with UV initiated surface grafting polymerization of acrylic monomers for obtaining different hydrophilicity, surface charge and reactive groups. DNA, avidin and biotin arrays were made through carboxylic patterns.

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Fast Development Strategy: One-Week-to-Chip
K.S.Dresse, G.Munchow and M.Ritzi

Mass manufacturing techniques like injection molding and lamination processes that allow the production of final disposable products at reasonable costs. What is needed is a process that allows fast tests of concepts and for the validation of the final chip design. As such tests pick up more and more speed the more you can rely on already established elements.

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Turning Valves for Lab-on-a-Chip Applications Enable Directional Flow and Portion Out Pre-Defined Volumes
R. Gransee, D. Dadic, F. Doffing, E. Schaeffer and K. S. Drese.

In this study the design and the realization of low-cost valves suitable for the integration into disposable polymer chips is presented. In contrast to commonly used sandwich-setups a plain design consisting of a simply structured polymer chip and a cover membrane is used.

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A Valve-Controlled Microfluidic Sysytem for Rapid Two-Dimensional Electrophoresis
Yiqi Luo, Bo Huang, Michael P. Bokoch, Richard N. Zare.

Two-dimensional electrophoresis of proteins is achieved in 500 seconds in a microfluidic system fabricated from PDMS, in which the first dimension of micellar electrokinetic chromatography and the second dimension of capillary sieving electrophoresis are coupled. By installing valves at the intersection of two dimensions, the separations are performed sequentially without interference.

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High Throughput Screening of Biocompatible Polymers
Guilhem A. Tourniaire, Jerome Sallette and Mark Bradley

The aim of this project was to develop high throughput screening methods using an IMSTAR Pathfinder™ High-content imaging platform to support the analyses of polymeric materials synthesised using a parallel approach. This was achieved by developing arrays of polymeric materials.

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Application of Microarrays in Process Analytical Technologies A First Step Towards Real Time Product Release
Streefland M, van de Waterbeemd B, vander Pol LA, Beuvery EC, Martens DE,Tramper J

Application of microarrays during process development helps to gain insight in biological processes involved in product formation, increasing process understanding.

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Gene Identification Through Array CGH: The CHARGE Syndrome
Lisenka Vissers, Conny van Ravenswaaij, Ronald Admiraal, Jane Hurst, Bert de Vries, Irene Janssen, Walter van der Vliet, Erik Huys, Pieter de Jong, Ben Hamel, Eric Schoenmakers, Han Brunner, Joris Veltman and Ad Geurts van Kessel.

In this study we have used high-resolution genome-wide DNA copy number screening by array-based comparative genomic hybridization (array CGH)2-3 to identify the underlying genetic cause of this malformation syndrome. Through this screening we identified overlapping microdeletions on chromosome 8 in two CHARGE patients.

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New 3D Black Substrate for Protein Microarrays with Improved Dynamic Range
Markus Hollas, Eric Jallerat, Karl Pflanz, Inka Praulich, Johanna Walter

A competitive study analysing the background fluorescence and the detectable concentration range of a chosen antigen is presented. An Affymetrix Spotting roboter 417 arrayer was used for protein dotting. The analysis is based on fluorescence scanning experiments with an Affymetrix 428 array scanner at a gain of 60. Data analysis was made with ImaGene Premium 5.5.3 by Biodiscovery Inc.

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Optimisation Workflow for the Production and Processing of High Quality Microarrays Applications in DNA, Peptide, antibody, and Carbohydrate Microarraying
Jens Sobek, Catharine Aquino and Ralph Schlapbach

We have developed a workflow for optimisation of array preparation and for processing protocols. Only 4 independent model experiments and solvent, immobilisation and blocking conditions. Optimal conditions or high quality microarray experiments were determined and used for array production and slide processing at the FGCZ.

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Showing Results 121 - 130 of 138
Scientific News
Rapid Test Kit Detects Dengue Antibodies from Saliva
IBN’s MedTech innovation simplifies diagnosis of infectious diseases.
UBC Engineers Develop Biochem Point-of-Care Lab for Smartphones
UBC Okanagan takes lab on a chip technology to a new inexpensive level.
New Way To Model Sickle Cell Behavior
Microfluidic device allows researchers to predict behavior of patients’ blood cells.
Watching How Cells Interact
New device allows scientists to glimpse communication between immune cells.
Extracting Tumor Cells From Blood
UCLA scientists use ‘NanoVelcro’ and temperature control to extract tumor cells from blood.
Sweeping Cells Apart For Use In Medical Research
Scientists have developed a new method to separate cells, which could lead to more efficient medical research.
How Bacteria Control Their Size
By monitoring thousands of individual bacteria scientists discovered how they maintain their size from generation to generation.
Tailor-Made Cancer Treatments? New Cell Culture Technique Paves The Way
Technique grew cells from 73% of patients in the study, more than three times as effective as previous methods.
Predicting Sepsis
Altered white-blood-cell motion in burn patients may warn of infection.
New Advance in Cryopreservation Could Change Management of World Blood Supplies
Engineers have identified a method to rapidly prepare frozen red blood cells for transfusions.
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