|Hepatitis B virus (HBV) and Human immunodeficiency virus (HIV) antibodies detected by peptide microarrays|
ahmed Abd El Wahed1, Ulrike Beutling2, Ronald Frank2, Gerhard Hunsmann1, and Hans-Joachim Fritz3
HBV and HIVenv chips with overlapping oligopeptides encompassing the full amino acid sequences of HBV and HIV polypeptides were produced. In addition, a chip displaying a library of random 4608 different 15-mers peptides (4608-RPL) was prepared. Both chips were used for analyzing monoclonal antibodies and sera from HIV- and HBV-infected individuals. 4608-RPL could be used for identifying target sequences of antibodies without prior knowledge of the corresponding immunizing antigen.
|System-level Simulation of Liquid Filling in Microfluidic Chips|
Hongjun Song, Yi Wang, and Kapil Pant
The overall objective of our work is to develop a system-level model and simulation framework for investigating the liquid filling process (including the filling time, filling pattern/status, flow velocity/pressure etc.) in complex microfluidic networks with order-of-magnitude speedup over the high-fidelity simulations and without appreciably compromising analysis accuracy.
|EXPERIMENTAL CHARACTERIZATION OF A METHOD TO REVERSIBLY BOND MICROFLUIDIC DEVICES THROUGH MAGNETIC FORCES|
Francesco Piraino, Matteo Moretti, Alberto Redaelli, Marco Rasponi
A method based on magnetic forces to reversibly bond glass slides to PDMS microfluidic devices has been developed and experimentally characterized. Results show a reliable tightness in normal laboratory applications.
|Pressure Driven HepG2 cells Focusing on a Microchip|
Paul-Emile POLENI, Olivier DUCLOUX, Serge OSTROVIDOV, Hiroyuki FUJITA, Teruo FUJII
We present a pressure driven microfluidic system devoted to concentration of cells in localized clusters ready for 3D cell culture in a microchamber. Cells are flown back and forth into the microchamber by pressurization/de-pressurization of two air caps symmetrically placed at two ends of a microchannel. The symmetry of the flow induces the presence of a dead volume in the cell culture chamber, favouring a rapid cell trapping and aggregation.
|Controlled Synthesis and Manipulation of Self-Assembled Peptide Nano Spheres by Microfluidic Dielectrophoresis (DEP)|
Nikolaj O. Christiansen, Mohammad Ajine, Jaime Castillo, Maria Dimaki & Winnie E. Svendsen
The self-assembled peptides as building blocks are excellent candidates for applications in biomedical nanotechnology because of their chemical diversity, flexibility, biocompatibility and stability. This work shows a stable synthesis in liquid and that is's posible to manipulate them using positive DEP.
|A Platform to Study Platelet Aggregation and Thrombus Growth Based on Dynamic Stress|
F. J. Tovar-Lopeza1, G. Rosengarten2, K. Khoshmanesh3 , E. Westein4 , S. P. Jackson4, Arnan Mitchell 1, and W. S. Nesbitt4
We present a microfluidic device that is able to trigger initial recruitment and subsequent aggregation of discoid platelets by mimicking the effects of pathological changes in blood vessel geometry.
|Development of a Fully Integrated Microfluidic Device for Electromodulated Liquid Chromatography with C4D Detection|
Jeremy Galineau, Blanaid White, Aoife Morrin, Malcolm R. Smyth
Applications for microfluidic technologies in life science are expanding rapidly, and have the potential to impact enormously across a range of
fields, from cell manipulation to separation science. In separation science their small channel dimensions make them ideal for high throughput
separations, reducing sample volumes and solvent consumption.
|A Practical Microfluidic Device for Synthesis of Purified Monodisperse Micro-Alginate Beads (MABs) as Microcarriers of Gold Nanoparticles.|
Paul-Emile POLENI, Serge OSTROVIDOV, Yasuyuki SAKAI and Teruo FUJII
We developed a microfluidic device for encapsulating gold nanoparticles in Micro-Alginate Beads (MABs). The size and the gap of the monodisperse alginate droplets were successfully controlled by adjusting the relative "oil/sample" flow rate ratio. Droplets reacted with calcium ion at the interface between oil phase and aqueous phase so that MABs precipitated spontaneously and undergone complete gelation. Purified MABs were successfully observed by fluorescence microscopy.
|ON CHIP PROTEIN DYNAMICS IN SINGLE BACTERIA CELLS WITH SPATIO-TEMPORAL RESOLUTION |
Dominik Greif, Nataliya Pobigaylo, Anke Becker, Jan Regtmeier and Dario Anselmetti
We demonstrate spatio-temporal protein dynamics in single living bacterial cells from time lapse fluorescence imaging (TLFI) in a microfluidic chip.