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Sequence-independent Selective Amplification of mRNAs over rRNAs
John Arrand1, Sim Sihota1, Wenbin Wei1 and Guido Krupp2

Standard mRNA amplifications for "All-Exon" microarrays and for bacterial RNAs are impossible with small samples and with degraded RNAs, because removal of rRNAs must precede universal, non-selective RNA amplification. This pre-treatment with magnetic beads is cumbersome, requires high amounts of starting material, is not universal for all species and degraded RNAs are not suitable.

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Novel Concept of Microarray Construction and their Application in Biology
B. Cegielska, M. K. Chmielewski, W.T. Markiewicz, M. Figlerowicz

For microarrays produced using spotting technologies, much attention has to be given to the development of slide surfaces, attachment chemistries, and spotting solutions. Application of the optimal and reliable methods ensuring effective binding of DNA probes with slide surface is one of the key factors warranting high quality results. Developments in the field of microarrays occur at a rapid pace and some novel approaches may offer suggestions of new strategies.

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Sub-classification of Colorectal Cancer Using Surface Antigen Antibody Microarray and Fluorescence Multiplexing
Jerry Zhou, Larissa Belov, Pauline Huang, Joo-Shik Shin, Michael J. Solomon, Pierre Chapuis, Les Bokey, Charles Chan and Richard I. Christopherson

Colorectal cancer (CRC) is the second most frequent cause of cancer deaths in Australia. Even after resection up to 50% of patients relapse. In an attempt to prevent recurrences chemotherapy is administered to high risk patients. However, as few as 10-20% patients genuinely benefit because the clinical course for individuals with CRC remains difficult to predict, largely due to prognostically heterogeneous groups within same-stage tumour categories.

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Hepatitis B virus (HBV) and Human immunodeficiency virus (HIV) antibodies detected by peptide microarrays
ahmed Abd El Wahed1, Ulrike Beutling2, Ronald Frank2, Gerhard Hunsmann1, and Hans-Joachim Fritz3

HBV and HIVenv chips with overlapping oligopeptides encompassing the full amino acid sequences of HBV and HIV polypeptides were produced. In addition, a chip displaying a library of random 4608 different 15-mers peptides (4608-RPL) was prepared. Both chips were used for analyzing monoclonal antibodies and sera from HIV- and HBV-infected individuals. 4608-RPL could be used for identifying target sequences of antibodies without prior knowledge of the corresponding immunizing antigen.

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System-level Simulation of Liquid Filling in Microfluidic Chips
Hongjun Song, Yi Wang, and Kapil Pant

The overall objective of our work is to develop a system-level model and simulation framework for investigating the liquid filling process (including the filling time, filling pattern/status, flow velocity/pressure etc.) in complex microfluidic networks with order-of-magnitude speedup over the high-fidelity simulations and without appreciably compromising analysis accuracy.

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EXPERIMENTAL CHARACTERIZATION OF A METHOD TO REVERSIBLY BOND MICROFLUIDIC DEVICES THROUGH MAGNETIC FORCES
Francesco Piraino, Matteo Moretti, Alberto Redaelli, Marco Rasponi

A method based on magnetic forces to reversibly bond glass slides to PDMS microfluidic devices has been developed and experimentally characterized. Results show a reliable tightness in normal laboratory applications.

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Pressure Driven HepG2 cells Focusing on a Microchip
Paul-Emile POLENI, Olivier DUCLOUX, Serge OSTROVIDOV, Hiroyuki FUJITA, Teruo FUJII

We present a pressure driven microfluidic system devoted to concentration of cells in localized clusters ready for 3D cell culture in a microchamber. Cells are flown back and forth into the microchamber by pressurization/de-pressurization of two air caps symmetrically placed at two ends of a microchannel. The symmetry of the flow induces the presence of a dead volume in the cell culture chamber, favouring a rapid cell trapping and aggregation.

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Controlled Synthesis and Manipulation of Self-Assembled Peptide Nano Spheres by Microfluidic Dielectrophoresis (DEP)
Nikolaj O. Christiansen, Mohammad Ajine, Jaime Castillo, Maria Dimaki & Winnie E. Svendsen

The self-assembled peptides as building blocks are excellent candidates for applications in biomedical nanotechnology because of their chemical diversity, flexibility, biocompatibility and stability. This work shows a stable synthesis in liquid and that is's posible to manipulate them using positive DEP.

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A Platform to Study Platelet Aggregation and Thrombus Growth Based on Dynamic Stress
F. J. Tovar-Lopeza1, G. Rosengarten2, K. Khoshmanesh3 , E. Westein4 , S. P. Jackson4, Arnan Mitchell 1, and W. S. Nesbitt4

We present a microfluidic device that is able to trigger initial recruitment and subsequent aggregation of discoid platelets by mimicking the effects of pathological changes in blood vessel geometry.

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Showing Results 41 - 50 of 137
Scientific News
New Advance in Cryopreservation Could Change Management of World Blood Supplies
Engineers have identified a method to rapidly prepare frozen red blood cells for transfusions.
Stanford Engineers Discover How to Record the Forensic History of Chemical Contaminations in Water
An invention called a time capsule is a tiny chemistry lab designed to take a fingerprint of contamination and also disclose when it occurred.
Wallet-Sized Labs The Next Big Thing
RMIT researchers are developing inexpensive, portable toxicology laboratories so small you could fit them in your wallet.
Device Invented at Johns Hopkins Provides Up-close Look at Cancer on the Move
Microscopic view of metastasis could give insight about how to keep cancer in check.
A Medical Lab For The Home
Fraunhofer FIT demonstrates a mobile wireless system that monitors the health of elderly people in their own homes, using miniature sensors.
Detecting Prostate Cancer With a Microfluidic Device
Innovative device detects prostate cancer, kidney disease on the spot.
Hello? Sweat and a Smartphone Could Become The Hot New Health Screening
A new article highlighting UC research reveals how sweat and microfluidics can pinpoint and help dodge potential health issues for everyone from athletes to preemies.
New 'Lab-on-a-Chip' Could Revolutionize Early Diagnosis of Cancer
Faster result times, reduced costs, minimal sample demands and better sensitivity of analysis.
How Fluid Flow Influences Neuron Growth
A University of Texas at Arlington team exploring how neuron growth can be controlled in the lab and, possibly, in the human body has published a new paper in Nature Scientific Reports on how fluid flow could play a significant role.
‘Tissue Chip’ to Screen Neurological Toxins
UW-Madison team are developing a faster, more affordable way to screen for neural toxins.
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