|Identification of differentially expressed transcripts associated to apomixis in B r a c h ia r ia using cDNA microarrays|
Eduardo Gorrón 1 , 2, Diana Bernal 1, Silvia Restrepo & Joe Tohme
Apomixis is a trait which allows flowering plants to produce seeds by asexual ways. Molecular mechanisms behind this phenomenon are poorly understood. We used cDNA microrrays coupled to substractive libraries to find genes related to apomixis in Brachiaria. Genes related to meiosis and cell division, and some putative transcription factors, were overexpressed in sexual plants. It may indicate that apomixis could be caused by downregulation of these genes.
|Design of an innovative microfluidic system to study chemotactic transmembranal migration of leukocytes |
Elena Bianchi (a)(b)(c), Elwin Vrouwe (b) , Laganà Katia (a) , Margherita Cioffi (a), Marko Blom (b), Bob Lansdorp (b), Gabriele Dubini (a)
Aim of this project is to develop a versatile and highthroughput microdevice, to be employed in studies of leukocytic chemotaxis, shear stress dependent.
|Perfecting Bacterial Tumor Treatment using Microfluidic Bioreactors|
Bhushan J. Toley, Brett M. Babin, Colin L. Walsh, Neil S. Forbes
Engineered bacteria provide a great opportunity to overcome the limitations of current cancer chemotherapeutics. We have developed microfluidic continuous flow-through devices as in-vitro models of tumor tissue and used them to quantify therapeutic efficacies of bacterial strains.
|A PDMS Sample Pre-treatment Device for the Optimization of Electrokinetic Manipulations of Serum|
Tim Abram, Dr. David Clague
A PDMS “sample pretreatment” device has been fabricated in order to selectively tune key biological sample parameters which will optimize the sample for subsequent electrokinetic manipulations. We have shown that a raw sample can be homogeneously combined with specific buffers in a DC pulse micromixer in under 1.5 seconds.
|A novel dynamic biochip platform for real-time detection and quantification of proteins|
M. Rendl, T. Brandstetter, J. Rühe
A proof of principle of a protein biochip platform permitting analysis of multiple clinically relevant proteins is presented.
|HIV POC testing by ssDNA coupled with NALF |
Natasha Gous, Lesley E. Scott, Alexio Capovilla, Natela Rekhviasvili, Wendy Stevens
A isothermal amplification termed Reverse Transcription Loop Dependant Amplification (RT-LDA) was developed with an affordable nucleic acid lateral flow detection (NALF) system, as one component of a potential POC HIV-1 RNA assay for subtypeC. RT-LDA makes use of a primer design that efficiently converts viral RNA into ssDNA amplicons, in 1 hour at 53ºC. Due to the single stranded nature of the product, the amplicon could be detected using NALF.
|Microfluidic assembly of magnetic gel particles|
C. H. Chen, A. R. Abate, D. Lee, E. M. Terentjev and D. A. Weitz
Monodisperse spherical magnetic gel particles containing asymmetric infrastructure were fabricated by a new microfluidics-based technique using double-emulsion droplet as templates. Double emulsions with functional cores and hydrogel shells were generated by the flow-focusing drop makers with special wettability patterning. Particles were made with a consistently anisotropic internal structure, which leads to their uniform anisotropy to perform the highly rotational controls by applying the magn
|Microfluidic PCR device for diagnostic pathogen detection|
Johannes R. Peham, Hannes Steiner, Walter Grienauer, Rudolf Heer, Michael J. Vellekoop, Christa Nöhammer, Herbert Wiesinger
In this work a microfluidic cyclic flow PCR device is presented, which is capable of replicating the bacterial genomic DNA sequence of the 16S ribosomal RNA. The standard laboratory processing time of 3 h could be decreased to 60 min with the microfluidic reactor without loosing PCR efficiency. Integrating an optical fluorescence detector for dsDNA measurement would evolve this device into a micro total analysis system.
|Design and Fabrication of a Micro PCR Module for POC Applications|
E. Morganti, C. Collini, C. Ress, A. Adami, L. Lorenzelli
The design and fabrication process of a micro PCR module is presented. The final system will be integrated in an innovative Lab on a Chip (LOC) to provide a technological platform able to detect autoimmune genetic diseases.