|A Practical Microfluidic Device for Synthesis of Purified Monodisperse Micro-Alginate Beads (MABs) as Microcarriers of Gold Nanoparticles.|
Paul-Emile POLENI, Serge OSTROVIDOV, Yasuyuki SAKAI and Teruo FUJII
We developed a microfluidic device for encapsulating gold nanoparticles in Micro-Alginate Beads (MABs). The size and the gap of the monodisperse alginate droplets were successfully controlled by adjusting the relative "oil/sample" flow rate ratio. Droplets reacted with calcium ion at the interface between oil phase and aqueous phase so that MABs precipitated spontaneously and undergone complete gelation. Purified MABs were successfully observed by fluorescence microscopy.
|ON CHIP PROTEIN DYNAMICS IN SINGLE BACTERIA CELLS WITH SPATIO-TEMPORAL RESOLUTION |
Dominik Greif, Nataliya Pobigaylo, Anke Becker, Jan Regtmeier and Dario Anselmetti
We demonstrate spatio-temporal protein dynamics in single living bacterial cells from time lapse fluorescence imaging (TLFI) in a microfluidic chip.
|Integration of predictive model with microfluidics fabrication using a 193 nm excimer laser source shaped by an Intelligent Pinhole mask|
Kevin Conlisk and G.M. O'Connor
Simulation, analysis and fabrication of microfluidic geometries using a laser-based fabrication process incorporating a reconfigurable mask. A LabVIEW development programme provides geometrical analysis prior to fabrication. The same programme is then used to control the mask during the machining step.
|Multifunctional, “Smart”, Polymeric Microfluidics Fabricated by Plasma Processing: Applications in Capillary Filling, and Passive Superhydrophobic Valving|
Katerina Tsougeni, Dimitris Papageorgiou, Angeliki Tserepi and Evangelos Gogolides*
We demonstrate a mass-production amenable technology for fabrication, surface modification and multifunction integration in plastic, disposable microfluidic devices, namely direct lithography on the plastic substrate followed by polymer plasma etching, and if desired by selective plasma deposition. We apply the plasma processing technology to fabricate polymeric microfluidics in Poly(methyl methacrylate) (PMMA) and Poly(ether ether ketone) (PEEK). Our approach proposes “smart” multifunctional mi
|In-plane detection of fluorescence signals in microfludic lab-on-chip flow cytyometry|
James Hoyland, Casper Kunstmann-Olsen
Several means of extracting fluorescence signals from flowing cell suspensions in a single plane are examined. Simple microfluidic flow cytometer structures incorporating lateral hydrodynamic focusing were molded in PDMS. Several geometries for embedding optical fibers and custom molded waveguides into the same structure were compared. Improvement in light yield is examined with molded cylindrical lenses and by using channels filled with high refractive index polymers as waveguides.
|Gene List Significance Index (GLSI) improves our method High Performance Chip Data Analysis dramatically - Quantifying the quality of different lists of analyzed significant genes|
Joachim R. Grün (1), Andreas Grützkau (1), Marta Steinbrich-Zöllner (3) Thomas Häupl (2), Ria Baumgrass (1), Jochen Sieper (3), Gerd-Rüdiger Burmester (2), Andreas Radbruch (1)
Our gene expression profiling strategy High Performance Chip Data Analysis (HPCDA) was improved with a method for quantifying the quality of different gene lists (GLs) with the new Gene List Significance Index (GLSI). With GLSI it is possible to decide which of two different extracted GLs has highest fraction of true positives, of high fold change or low p-value genes. With GLSI we could empirically optimize HPCDA-Score for ranking genes.
|PROTEOME WIDE PLASMA PROFILING USING ANTIBODY SUSPENSION BEAD ARRAYS|
Maja Neiman, Ulrika Igel, Burcu Ayoglu, Kimi Drobin, Mathias Uhlén, Peter Nilsson and Jochen M. Schwenk
A newly developed antibody suspension bead array assay allows for a systematic and high-throughput plasma profiling. This microtiter based assay uses antibody-coupled beads for a multiplexed analysis of minute amounts of directly labelled samples. The key requirement of a?nity reagents towards all human proteins is met by the Human Protein Atlas project.
|Evaluation of microfluidic digital PCR for the detection of cancer biomarkers|
Rebecca Sanders, Claire Bushell, Carole Foy, Daniel J. Scott
dPCR is achieved by sample partitioning prior to PCR amplification such that each reaction chamber contains one copy or less of target DNA. This dilution becomes the limiting factor and an accurate target molecule count is achievable. This study evaluates dPCR’s quantitative capabilities and investigates parameters influencing copy number quantification, using the Fluidigm Biomark instrument. Biomark technology combines dPCR theory with a microfluidics platform.
|Identification of differentially expressed transcripts associated to apomixis in B r a c h ia r ia using cDNA microarrays|
Eduardo Gorrón 1 , 2, Diana Bernal 1, Silvia Restrepo & Joe Tohme
Apomixis is a trait which allows flowering plants to produce seeds by asexual ways. Molecular mechanisms behind this phenomenon are poorly understood. We used cDNA microrrays coupled to substractive libraries to find genes related to apomixis in Brachiaria. Genes related to meiosis and cell division, and some putative transcription factors, were overexpressed in sexual plants. It may indicate that apomixis could be caused by downregulation of these genes.