|Design of an innovative microfluidic system to study chemotactic transmembranal migration of leukocytes |
Elena Bianchi (a)(b)(c), Elwin Vrouwe (b) , Laganà Katia (a) , Margherita Cioffi (a), Marko Blom (b), Bob Lansdorp (b), Gabriele Dubini (a)
Aim of this project is to develop a versatile and highthroughput microdevice, to be employed in studies of leukocytic chemotaxis, shear stress dependent.
|Perfecting Bacterial Tumor Treatment using Microfluidic Bioreactors|
Bhushan J. Toley, Brett M. Babin, Colin L. Walsh, Neil S. Forbes
Engineered bacteria provide a great opportunity to overcome the limitations of current cancer chemotherapeutics. We have developed microfluidic continuous flow-through devices as in-vitro models of tumor tissue and used them to quantify therapeutic efficacies of bacterial strains.
|A PDMS Sample Pre-treatment Device for the Optimization of Electrokinetic Manipulations of Serum|
Tim Abram, Dr. David Clague
A PDMS “sample pretreatment” device has been fabricated in order to selectively tune key biological sample parameters which will optimize the sample for subsequent electrokinetic manipulations. We have shown that a raw sample can be homogeneously combined with specific buffers in a DC pulse micromixer in under 1.5 seconds.
|A novel dynamic biochip platform for real-time detection and quantification of proteins|
M. Rendl, T. Brandstetter, J. Rühe
A proof of principle of a protein biochip platform permitting analysis of multiple clinically relevant proteins is presented.
|HIV POC testing by ssDNA coupled with NALF |
Natasha Gous, Lesley E. Scott, Alexio Capovilla, Natela Rekhviasvili, Wendy Stevens
A isothermal amplification termed Reverse Transcription Loop Dependant Amplification (RT-LDA) was developed with an affordable nucleic acid lateral flow detection (NALF) system, as one component of a potential POC HIV-1 RNA assay for subtypeC. RT-LDA makes use of a primer design that efficiently converts viral RNA into ssDNA amplicons, in 1 hour at 53ºC. Due to the single stranded nature of the product, the amplicon could be detected using NALF.
|Microfluidic assembly of magnetic gel particles|
C. H. Chen, A. R. Abate, D. Lee, E. M. Terentjev and D. A. Weitz
Monodisperse spherical magnetic gel particles containing asymmetric infrastructure were fabricated by a new microfluidics-based technique using double-emulsion droplet as templates. Double emulsions with functional cores and hydrogel shells were generated by the flow-focusing drop makers with special wettability patterning. Particles were made with a consistently anisotropic internal structure, which leads to their uniform anisotropy to perform the highly rotational controls by applying the magn
|Microfluidic PCR device for diagnostic pathogen detection|
Johannes R. Peham, Hannes Steiner, Walter Grienauer, Rudolf Heer, Michael J. Vellekoop, Christa Nöhammer, Herbert Wiesinger
In this work a microfluidic cyclic flow PCR device is presented, which is capable of replicating the bacterial genomic DNA sequence of the 16S ribosomal RNA. The standard laboratory processing time of 3 h could be decreased to 60 min with the microfluidic reactor without loosing PCR efficiency. Integrating an optical fluorescence detector for dsDNA measurement would evolve this device into a micro total analysis system.
|Design and Fabrication of a Micro PCR Module for POC Applications|
E. Morganti, C. Collini, C. Ress, A. Adami, L. Lorenzelli
The design and fabrication process of a micro PCR module is presented. The final system will be integrated in an innovative Lab on a Chip (LOC) to provide a technological platform able to detect autoimmune genetic diseases.
|Fabrication and characterization of a fully integrated microdevice for in-vitro single cell assays|
C. Collini, E. Morganti, R. Cunaccia, L. Odorizzi, C. Ress, and L. Lorenzelli (1), A. De Toni, G. Marinaro (2), M. Borgo (3), M. Maschietto (4)
This work presents the microfabrication and preliminary characterization of a fully integrated microdevice for in-vitro single cell assays. This technological platform combines IDEs and MEA-based modules for cell addressed delivery of bio-functionalized nano/microparticles and single cell electroporation respectively.