|Pressure Driven HepG2 cells Focusing on a Microchip|
Paul-Emile POLENI, Olivier DUCLOUX, Serge OSTROVIDOV, Hiroyuki FUJITA, Teruo FUJII
We present a pressure driven microfluidic system devoted to concentration of cells in localized clusters ready for 3D cell culture in a microchamber. Cells are flown back and forth into the microchamber by pressurization/de-pressurization of two air caps symmetrically placed at two ends of a microchannel. The symmetry of the flow induces the presence of a dead volume in the cell culture chamber, favouring a rapid cell trapping and aggregation.
|Controlled Synthesis and Manipulation of Self-Assembled Peptide Nano Spheres by Microfluidic Dielectrophoresis (DEP)|
Nikolaj O. Christiansen, Mohammad Ajine, Jaime Castillo, Maria Dimaki & Winnie E. Svendsen
The self-assembled peptides as building blocks are excellent candidates for applications in biomedical nanotechnology because of their chemical diversity, flexibility, biocompatibility and stability. This work shows a stable synthesis in liquid and that is's posible to manipulate them using positive DEP.
|A Platform to Study Platelet Aggregation and Thrombus Growth Based on Dynamic Stress|
F. J. Tovar-Lopeza1, G. Rosengarten2, K. Khoshmanesh3 , E. Westein4 , S. P. Jackson4, Arnan Mitchell 1, and W. S. Nesbitt4
We present a microfluidic device that is able to trigger initial recruitment and subsequent aggregation of discoid platelets by mimicking the effects of pathological changes in blood vessel geometry.
|Development of a Fully Integrated Microfluidic Device for Electromodulated Liquid Chromatography with C4D Detection|
Jeremy Galineau, Blanaid White, Aoife Morrin, Malcolm R. Smyth
Applications for microfluidic technologies in life science are expanding rapidly, and have the potential to impact enormously across a range of
fields, from cell manipulation to separation science. In separation science their small channel dimensions make them ideal for high throughput
separations, reducing sample volumes and solvent consumption.
|A Practical Microfluidic Device for Synthesis of Purified Monodisperse Micro-Alginate Beads (MABs) as Microcarriers of Gold Nanoparticles.|
Paul-Emile POLENI, Serge OSTROVIDOV, Yasuyuki SAKAI and Teruo FUJII
We developed a microfluidic device for encapsulating gold nanoparticles in Micro-Alginate Beads (MABs). The size and the gap of the monodisperse alginate droplets were successfully controlled by adjusting the relative "oil/sample" flow rate ratio. Droplets reacted with calcium ion at the interface between oil phase and aqueous phase so that MABs precipitated spontaneously and undergone complete gelation. Purified MABs were successfully observed by fluorescence microscopy.
|ON CHIP PROTEIN DYNAMICS IN SINGLE BACTERIA CELLS WITH SPATIO-TEMPORAL RESOLUTION |
Dominik Greif, Nataliya Pobigaylo, Anke Becker, Jan Regtmeier and Dario Anselmetti
We demonstrate spatio-temporal protein dynamics in single living bacterial cells from time lapse fluorescence imaging (TLFI) in a microfluidic chip.
|Integration of predictive model with microfluidics fabrication using a 193 nm excimer laser source shaped by an Intelligent Pinhole mask|
Kevin Conlisk and G.M. O'Connor
Simulation, analysis and fabrication of microfluidic geometries using a laser-based fabrication process incorporating a reconfigurable mask. A LabVIEW development programme provides geometrical analysis prior to fabrication. The same programme is then used to control the mask during the machining step.
|Multifunctional, “Smart”, Polymeric Microfluidics Fabricated by Plasma Processing: Applications in Capillary Filling, and Passive Superhydrophobic Valving|
Katerina Tsougeni, Dimitris Papageorgiou, Angeliki Tserepi and Evangelos Gogolides*
We demonstrate a mass-production amenable technology for fabrication, surface modification and multifunction integration in plastic, disposable microfluidic devices, namely direct lithography on the plastic substrate followed by polymer plasma etching, and if desired by selective plasma deposition. We apply the plasma processing technology to fabricate polymeric microfluidics in Poly(methyl methacrylate) (PMMA) and Poly(ether ether ketone) (PEEK). Our approach proposes “smart” multifunctional mi
|In-plane detection of fluorescence signals in microfludic lab-on-chip flow cytyometry|
James Hoyland, Casper Kunstmann-Olsen
Several means of extracting fluorescence signals from flowing cell suspensions in a single plane are examined. Simple microfluidic flow cytometer structures incorporating lateral hydrodynamic focusing were molded in PDMS. Several geometries for embedding optical fibers and custom molded waveguides into the same structure were compared. Improvement in light yield is examined with molded cylindrical lenses and by using channels filled with high refractive index polymers as waveguides.