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Monday, September 22, 2014
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PROTEOME WIDE PLASMA PROFILING USING ANTIBODY SUSPENSION BEAD ARRAYS
Maja Neiman, Ulrika Igel, Burcu Ayoglu, Kimi Drobin, Mathias Uhlén, Peter Nilsson and Jochen M. Schwenk

A newly developed antibody suspension bead array assay allows for a systematic and high-throughput plasma profiling. This microtiter based assay uses antibody-coupled beads for a multiplexed analysis of minute amounts of directly labelled samples. The key requirement of a?nity reagents towards all human proteins is met by the Human Protein Atlas project.

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Evaluation of microfluidic digital PCR for the detection of cancer biomarkers
Rebecca Sanders, Claire Bushell, Carole Foy, Daniel J. Scott

dPCR is achieved by sample partitioning prior to PCR amplification such that each reaction chamber contains one copy or less of target DNA. This dilution becomes the limiting factor and an accurate target molecule count is achievable. This study evaluates dPCR’s quantitative capabilities and investigates parameters influencing copy number quantification, using the Fluidigm Biomark instrument. Biomark technology combines dPCR theory with a microfluidics platform.

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Identification of differentially expressed transcripts associated to apomixis in B r a c h ia r ia using cDNA microarrays
Eduardo Gorrón 1 , 2, Diana Bernal 1, Silvia Restrepo & Joe Tohme

Apomixis is a trait which allows flowering plants to produce seeds by asexual ways. Molecular mechanisms behind this phenomenon are poorly understood. We used cDNA microrrays coupled to substractive libraries to find genes related to apomixis in Brachiaria. Genes related to meiosis and cell division, and some putative transcription factors, were overexpressed in sexual plants. It may indicate that apomixis could be caused by downregulation of these genes.

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Design of an innovative microfluidic system to study chemotactic transmembranal migration of leukocytes
Elena Bianchi (a)(b)(c), Elwin Vrouwe (b) , Laganà Katia (a) , Margherita Cioffi (a), Marko Blom (b), Bob Lansdorp (b), Gabriele Dubini (a)

Aim of this project is to develop a versatile and highthroughput microdevice, to be employed in studies of leukocytic chemotaxis, shear stress dependent.

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Perfecting Bacterial Tumor Treatment using Microfluidic Bioreactors
Bhushan J. Toley, Brett M. Babin, Colin L. Walsh, Neil S. Forbes

Engineered bacteria provide a great opportunity to overcome the limitations of current cancer chemotherapeutics. We have developed microfluidic continuous flow-through devices as in-vitro models of tumor tissue and used them to quantify therapeutic efficacies of bacterial strains.

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A PDMS Sample Pre-treatment Device for the Optimization of Electrokinetic Manipulations of Serum
Tim Abram, Dr. David Clague

A PDMS “sample pretreatment” device has been fabricated in order to selectively tune key biological sample parameters which will optimize the sample for subsequent electrokinetic manipulations. We have shown that a raw sample can be homogeneously combined with specific buffers in a DC pulse micromixer in under 1.5 seconds.

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A novel dynamic biochip platform for real-time detection and quantification of proteins
M. Rendl, T. Brandstetter, J. Rühe

A proof of principle of a protein biochip platform permitting analysis of multiple clinically relevant proteins is presented.

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HIV POC testing by ssDNA coupled with NALF
Natasha Gous, Lesley E. Scott, Alexio Capovilla, Natela Rekhviasvili, Wendy Stevens

A isothermal amplification termed Reverse Transcription Loop Dependant Amplification (RT-LDA) was developed with an affordable nucleic acid lateral flow detection (NALF) system, as one component of a potential POC HIV-1 RNA assay for subtypeC. RT-LDA makes use of a primer design that efficiently converts viral RNA into ssDNA amplicons, in 1 hour at 53ºC. Due to the single stranded nature of the product, the amplicon could be detected using NALF.

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Microfluidic assembly of magnetic gel particles
C. H. Chen, A. R. Abate, D. Lee, E. M. Terentjev and D. A. Weitz

Monodisperse spherical magnetic gel particles containing asymmetric infrastructure were fabricated by a new microfluidics-based technique using double-emulsion droplet as templates. Double emulsions with functional cores and hydrogel shells were generated by the flow-focusing drop makers with special wettability patterning. Particles were made with a consistently anisotropic internal structure, which leads to their uniform anisotropy to perform the highly rotational controls by applying the magn

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Showing Results 51 - 60 of 131
Scientific News
Blood Cleanser for Sepsis
Dialysis-like ‘biospleen’ quickly filters bacteria, fungi and toxins.
Stanford Engineers Aim to Connect the World with Ant-sized Radios
Costing just pennies to make, tiny radios-on-a-chip are designed to serve as controllers or sensors for the 'Internet of Things.'
Lab on a Breathing Chip
Human nasal epithelial cells, cultured on a microchip, react to air pollutants just like they would in the upper airway.
Micropumps for Lab-on-a-Chip Disease Diagnosis
Reliable, inexpensive, programmable pumps could help make the diagnosis of many global life-threatening diseases easy and affordable.
Novel Chip-based Platform Could Simplify Measurements of Single Molecules
A nanopore-gated optofluidic chip combines electrical and optical measurements of single molecules onto a single platform.
Ingested Nanoparticles May Damage Liver
Although nanoparticles in food, sunscreen and other everyday products have many benefits, researchers from Cornell are finding that at certain doses, the particles might cause human organ damage.
Single-Cell Analysis Holds Promise for Stem Cell and Cancer Research
UCSF researchers use microfluidic technology to probe human brain development.
Researchers Invent Nanotech Microchip to Diagnose Type-1 Diabetes
The cheap, portable, microchip-based test could speed up diagnosis and enable studies of how the disease develops.
Revealing the Role of “Precocious” Dendritic Cells in Inflammatory Response
Discovery makes it possible for researchers to explore how these “precocious” cells escalate the body’s immune response when the body is under attack.
New NIST Metamaterial Gives Light a One-Way Ticket
The device could someday play a role in optical information processing and in novel biosensing devices.
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