|Experimental Determination of ADMET Parameters in High Throughput, Using Colloidal Gold Composites and a Conductive Polymer as Reporting Reagents|
Roberto Martinez-Neira, Patricia De Pril, Anne Van Hoonacker, Patrick Englebienne
We developed and validated a high throughput in vitro setting to experimentally determine hSA and Orosomucoid affinities, fraction absorbed, Log BBB and Redox potential of NCEs using gold nanoparticles functionalized with proteins, lipids or conductive polymers. Such automated ADME assays provide means for more objective decisions in early drug discovery.
|TOWARDS MULTI-ANALYTE BIOSENSOR FOR FOOD SCREENING BASED ON IBIS IMAGING SPR|
S. Rebe Raz , M. Bremer, W. Norde
Our aim is to develop a dedicated tool for multi-analyte detection in food based on IBIS Surface Plasmon Resonance imaging platform using a microarray format. Utilizing a multi-immunoassay on top of the SPR- imaging platform will allow rapid and simultaneous detection of various food ingredients and contaminants (e.g. food allergens, residues of veterinary drugs and environmental contaminants), providing the end user with a detailed food profile.
|Combination of a transient assay based ChIP technology and transcriptome analyses for the exploration of potential transcription factor binding sites|
Shu-Jen Chou (presenter), Li-Teh Chen, Yi-Hang Li, Chiung-swey Joanne Chang and Shu-Hsing Wu
1. Combination of protoplast transient expression system and Nickel resin based purification is an useful approach for chromatin enrichment that can be used to identify transcription factor’s potential target genes. 2.Comparison our ChIP-on-chip target genes with in vivo binding targets using two whole genome tiling array platforms showing high degree of overlap between two methods.
|Phase Diagram Visualization via Continuously-Fed Crystallization: Experiments and Model|
Masano Sugiyama and Victor Barocas
A continuously-fed crystallization chamber is manufactured to allow phase diagram visualization. This microfluidic system allows the experimenter to screen a large range of salt and protein concentrations in one experiment. This allows the experimenter to predict the protein phase diagram in a single experiment. A continuous-feed crystallization chamber has been successfully fabricated and characterized in terms of its flow profile, and has successfully predicted the phase diagram for lysozyme.
|Microfluidic sensor device for online haemostasis monitoring|
L. Müller, A. Sterck, R. Gronmaier, S. Sinn, D. Klar, S. Haeberle, R. Zengerle, H.P. Wendel, H. Northoff, F. Gehring
We have developed a microfluidic chip that allows online haemostasis monitoring using a thickness shear mode sensor. The sensor allows the detection of adsorbed masses and changes in viscosity in real-time. The microfluidic mixer permits fast mixing of whole blood with activators, which trigger the coagulation cascade. Changes in frequency were measured during the coagulation of whole blood.
|A microfluidic approach for the directed evolution of proteins by retroviral display|
Lucia Granieri, Jean-Christophe Baret, Andrew D. Griffiths and Christoph A. Merten
The model system used here is based on retroviral particles displaying tPA, a protein used in current emergency therapies of myocardial infarction and stroke. Single tPA variants were encapsulated into aqueous droplets, at a frequency of ~10Kilohertz and the enzymatic activity was monitored using a fluorescence assay. Active variants could be clearly distinguished from inactive variants or variants incubated with the endogenous inhibitor PAI-1.
|A high-throughput protein array-based approach |
Rachel Van Dyk, Claudia Kirisits, Paul Potter, Fook Tim Chew, Reinhard Hiller
Here we present a novel discovery-oriented high-throughput approach to the screening of allergen reactivates in crude biological extracts is presented using seafood allergens as an example. The CPGR workflow permits the effective screening of hundreds of putative allergens in parallel using minute amounts of patient serum and constitutes a cost-efficient allergen-specific antibody screening method for a routine diagnostic setting.
|Development of a High-Throughput Microarray for Evaluating CYP1A1 Induction|
Megan Mason, XinXin Ding, Jonathan S. Dordick
The aim of this study is to produce a vector capable of expressing green fluorescent protein (GFP) when exposed to a CYP1A1 inducer.
|Bio-analytic silicon chips for the detection of developmentalneurotoxic effects of chemicals and drugs in the context of the European REACH program|
S. Buehler , Helene Altrichter , P. J. Koester , C. Tautorat , Ralf Ehret ,Werner Baumann , Jan Gimsa
Bio-analytic silicon chips can be used to investigate stem cell differentiation into neurons and for the in vitro on-line monitoring of cellular reactions under well controlled experimental conditions. Chip systems allow for a parallel, label-free and non-invasive measurement of different parameters by CMOS silicon microsensors by the application of potential neurotoxic and developmental neurotoxic substances.