|Bio-analytic silicon chips for the detection of developmentalneurotoxic effects of chemicals and drugs in the context of the European REACH program|
S. Buehler , Helene Altrichter , P. J. Koester , C. Tautorat , Ralf Ehret ,Werner Baumann , Jan Gimsa
Bio-analytic silicon chips can be used to investigate stem cell differentiation into neurons and for the in vitro on-line monitoring of cellular reactions under well controlled experimental conditions. Chip systems allow for a parallel, label-free and non-invasive measurement of different parameters by CMOS silicon microsensors by the application of potential neurotoxic and developmental neurotoxic substances.
|Modular Glass Chip System for the Acquisition of the Electric Activity and Physiological Parameters of Differentiated Stem Cells|
P. J. Koester, J. Sakowski, S. M. Buehler, C. Tautorat, H. Altrichter, W. Baumann and J. Gimsa
The EU chemical policy REACH, effective on June 2007, regulates the registration, evaluation and authorization of chemicals. Investigations on the effects of neurotoxic and developmental-neurotoxic substances are required by European and US-American test guidelines. However, at the moment these tests are based on animal experiments contradicting society claims to abolish animal experiments and to implement the 3R-principle. The resulting problem is… How to pair REACH and the 3Rs?
|New Microarray Substrate for Fluorescent Signal Enhancement|
Wayne Moore, Svetlana I. Novikova, David Moore, Lisa Jolin and Cindy Smith.
Microcosm BioBright slides (patent pending) are designed as a plug-and-play alternative to commercially available microarray substrates for use with different microarray platforms (oligo, miRNA or protein).
|Interactive Transcription Maps Over Microarray Data|
Petr Krontorad, Irena Koutna and Michal Strehovsky
For final description of microarray experiment results it is necessary to use some statistically robust method. Transcription maps (TM) are used to map results of gene expression analysis into individual chromosomes. We have implemented graphical tool for interactive display of TMs. This tool allows displaying of arbitrary information for each gene.
|Expression Profiling of CD34+ of Peripheral Blood of Patients with Lymphoma During ex vivo Granulocytic Differentiation|
Irena Koutna, Lenka Tesarova, Martin Klabusay, Petr Krontorad, Michal Strehovsky, Martina Peterkova and Viera Hrabcakova
On the basis of global expression data analysis we have concluded that population of CD34+ cells from patients with lymphoma shows different expression status during ex-vivo differentiation. And thus we assume that CD34+ population is not suitable for autologous transplantation. We have used cDNA microarrays technology to obtained precise expression profiles.
|Caveolin-1 Expression as a Possible Biomarker in Pancreatic Cancer Diagnosis|
C. Tanase, E. Raducan, L. Albulescu, E. Codorean, M.I. Nicolescu, D.I. Popescu, M.L. Cruceru and A.C. Popa
Caveolin1 (Cav-1) function either as a tumor supressor or as a promoter of metastasis. Overexpresion of cav-1 was correlated with: tumoral grading, proliferration markers (Ki67, p53), serum tumor markers (CEA, CA19.9) and angiogenic markers (VEGF, bFGF).
|Lab-on-a-chip Biomolecular Interaction Measurement System with Integrated Microfluidics and Surface Plasmon Resonance Imaging|
Ganeshram Krishnamoorthy, E.T. Carlen, S. Schlautmann, H.L. de Boer, A. Van den Berg and R. B. M. Schasfoort
We present a novel lab-on-a-chip approach for the measurement of the biomolecular interaction of a common model system (IgG – Anti IgG) using a commercial SPR imaging system (IBIS-iSPR, IBIS Technologies b.v., Hengelo, the Netherlands).
|A Novel Miniaturized Cell Lysis Device Using Spherically Focused Ultrasound|
Gang Li, Hong Xiao, Min Guo and Jing Cheng
A prototype of miniaturized cell lysis device has been developed using a spherically concave transducer, which is capable of lysing bacteria in absence of added chemical denaturants or enzymes and lysing yeast efficiently without any mechanical or enzymatic pretreatment.
|A Sandwich-Injection Method for Microchip Electrophoresis|
Gang Li, Gui-Sheng Zhuang, Hai-Bin Zhu, Hui-Qing Jin and Jian-Long Zhao
We present a sandwich-injection method for controlling discrete sample injection in µ-CE. This method involves four accessory arm channels in which symmetrical potentials are loaded to form a unique parallel electric field distribution to confine the spreading of sample plugs and prevent sample leakage. The virtues of the novel injection technique were demonstrated with numerical models and experiments.