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HIV POC testing by ssDNA coupled with NALF
Natasha Gous, Lesley E. Scott, Alexio Capovilla, Natela Rekhviasvili, Wendy Stevens

A isothermal amplification termed Reverse Transcription Loop Dependant Amplification (RT-LDA) was developed with an affordable nucleic acid lateral flow detection (NALF) system, as one component of a potential POC HIV-1 RNA assay for subtypeC. RT-LDA makes use of a primer design that efficiently converts viral RNA into ssDNA amplicons, in 1 hour at 53ºC. Due to the single stranded nature of the product, the amplicon could be detected using NALF.

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Microfluidic assembly of magnetic gel particles
C. H. Chen, A. R. Abate, D. Lee, E. M. Terentjev and D. A. Weitz

Monodisperse spherical magnetic gel particles containing asymmetric infrastructure were fabricated by a new microfluidics-based technique using double-emulsion droplet as templates. Double emulsions with functional cores and hydrogel shells were generated by the flow-focusing drop makers with special wettability patterning. Particles were made with a consistently anisotropic internal structure, which leads to their uniform anisotropy to perform the highly rotational controls by applying the magn

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Microfluidic PCR device for diagnostic pathogen detection
Johannes R. Peham, Hannes Steiner, Walter Grienauer, Rudolf Heer, Michael J. Vellekoop, Christa Nöhammer, Herbert Wiesinger

In this work a microfluidic cyclic flow PCR device is presented, which is capable of replicating the bacterial genomic DNA sequence of the 16S ribosomal RNA. The standard laboratory processing time of 3 h could be decreased to 60 min with the microfluidic reactor without loosing PCR efficiency. Integrating an optical fluorescence detector for dsDNA measurement would evolve this device into a micro total analysis system.

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Design and Fabrication of a Micro PCR Module for POC Applications
E. Morganti, C. Collini, C. Ress, A. Adami, L. Lorenzelli

The design and fabrication process of a micro PCR module is presented. The final system will be integrated in an innovative Lab on a Chip (LOC) to provide a technological platform able to detect autoimmune genetic diseases.

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Fabrication and characterization of a fully integrated microdevice for in-vitro single cell assays
C. Collini, E. Morganti, R. Cunaccia, L. Odorizzi, C. Ress, and L. Lorenzelli (1), A. De Toni, G. Marinaro (2), M. Borgo (3), M. Maschietto (4)

This work presents the microfabrication and preliminary characterization of a fully integrated microdevice for in-vitro single cell assays. This technological platform combines IDEs and MEA-based modules for cell addressed delivery of bio-functionalized nano/microparticles and single cell electroporation respectively.

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The Hind Sight of Nanotechnology
Suman Lahiri, Mamta Shah

Nanotechnology addresses manipulation and study of structures and devices with length scales in the 1- to 100-nanometers range. Market growing upto 1 to 2.95 trillion $ by 2015, projects nanotubes to cross US$5 billion by the year 2012. In view of the toxicity that can be posed by it serious problems can threaten to neutralize the gains of nanotechnology.

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Experimental Determination of ADMET Parameters in High Throughput, Using Colloidal Gold Composites and a Conductive Polymer as Reporting Reagents
Roberto Martinez-Neira, Patricia De Pril, Anne Van Hoonacker, Patrick Englebienne

We developed and validated a high throughput in vitro setting to experimentally determine hSA and Orosomucoid affinities, fraction absorbed, Log BBB and Redox potential of NCEs using gold nanoparticles functionalized with proteins, lipids or conductive polymers. Such automated ADME assays provide means for more objective decisions in early drug discovery.

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TOWARDS MULTI-ANALYTE BIOSENSOR FOR FOOD SCREENING BASED ON IBIS IMAGING SPR
S. Rebe Raz , M. Bremer, W. Norde

Our aim is to develop a dedicated tool for multi-analyte detection in food based on IBIS Surface Plasmon Resonance imaging platform using a microarray format. Utilizing a multi-immunoassay on top of the SPR- imaging platform will allow rapid and simultaneous detection of various food ingredients and contaminants (e.g. food allergens, residues of veterinary drugs and environmental contaminants), providing the end user with a detailed food profile.

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Combination of a transient assay based ChIP technology and transcriptome analyses for the exploration of potential transcription factor binding sites
Shu-Jen Chou (presenter), Li-Teh Chen, Yi-Hang Li, Chiung-swey Joanne Chang and Shu-Hsing Wu

1. Combination of protoplast transient expression system and Nickel resin based purification is an useful approach for chromatin enrichment that can be used to identify transcription factor’s potential target genes. 2.Comparison our ChIP-on-chip target genes with in vivo binding targets using two whole genome tiling array platforms showing high degree of overlap between two methods.

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Showing Results 61 - 70 of 134
Scientific News
New 'Lab-on-a-Chip' Could Revolutionize Early Diagnosis of Cancer
Faster result times, reduced costs, minimal sample demands and better sensitivity of analysis.
How Fluid Flow Influences Neuron Growth
A University of Texas at Arlington team exploring how neuron growth can be controlled in the lab and, possibly, in the human body has published a new paper in Nature Scientific Reports on how fluid flow could play a significant role.
‘Tissue Chip’ to Screen Neurological Toxins
UW-Madison team are developing a faster, more affordable way to screen for neural toxins.
Modular Components Make Building 3-D “Labs-on-a-Chip” a Snap
New building blocks take microfluidics from flat to 3-D quickly and easily.
NIH Funds Next Phase of Tissue Chip for Drug Screening Program
Scientists will integrate chips mimicking human organ functions into full body system to evaluate drugs.
Airway Muscle-On-A-Chip Mimics Asthma
Tissue-level model of human airway musculature could pave way for patient-specific asthma treatments.
New Chip Promising For Tumor-Targeting Research
The new system, called a tumor-microenvironment-on-chip device, will allow researchers to study the complex environment surrounding tumors and the barriers that prevent the targeted delivery of therapeutic agents.
Gene Expression Patterns in Pancreatic CTCs Revealed
Distinct patterns of gene expression in several groups of CTCs were identified, including significant differences from the primary tumor that may contribute to the ability to generate metastases and prove to be targets for improved treatment of the deadly tumor.
Blood Cleanser for Sepsis
Dialysis-like ‘biospleen’ quickly filters bacteria, fungi and toxins.
Stanford Engineers Aim to Connect the World with Ant-sized Radios
Costing just pennies to make, tiny radios-on-a-chip are designed to serve as controllers or sensors for the 'Internet of Things.'
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