|HIV POC testing by ssDNA coupled with NALF |
Natasha Gous, Lesley E. Scott, Alexio Capovilla, Natela Rekhviasvili, Wendy Stevens
A isothermal amplification termed Reverse Transcription Loop Dependant Amplification (RT-LDA) was developed with an affordable nucleic acid lateral flow detection (NALF) system, as one component of a potential POC HIV-1 RNA assay for subtypeC. RT-LDA makes use of a primer design that efficiently converts viral RNA into ssDNA amplicons, in 1 hour at 53ºC. Due to the single stranded nature of the product, the amplicon could be detected using NALF.
|Microfluidic assembly of magnetic gel particles|
C. H. Chen, A. R. Abate, D. Lee, E. M. Terentjev and D. A. Weitz
Monodisperse spherical magnetic gel particles containing asymmetric infrastructure were fabricated by a new microfluidics-based technique using double-emulsion droplet as templates. Double emulsions with functional cores and hydrogel shells were generated by the flow-focusing drop makers with special wettability patterning. Particles were made with a consistently anisotropic internal structure, which leads to their uniform anisotropy to perform the highly rotational controls by applying the magn
|Microfluidic PCR device for diagnostic pathogen detection|
Johannes R. Peham, Hannes Steiner, Walter Grienauer, Rudolf Heer, Michael J. Vellekoop, Christa Nöhammer, Herbert Wiesinger
In this work a microfluidic cyclic flow PCR device is presented, which is capable of replicating the bacterial genomic DNA sequence of the 16S ribosomal RNA. The standard laboratory processing time of 3 h could be decreased to 60 min with the microfluidic reactor without loosing PCR efficiency. Integrating an optical fluorescence detector for dsDNA measurement would evolve this device into a micro total analysis system.
|Design and Fabrication of a Micro PCR Module for POC Applications|
E. Morganti, C. Collini, C. Ress, A. Adami, L. Lorenzelli
The design and fabrication process of a micro PCR module is presented. The final system will be integrated in an innovative Lab on a Chip (LOC) to provide a technological platform able to detect autoimmune genetic diseases.
|Fabrication and characterization of a fully integrated microdevice for in-vitro single cell assays|
C. Collini, E. Morganti, R. Cunaccia, L. Odorizzi, C. Ress, and L. Lorenzelli (1), A. De Toni, G. Marinaro (2), M. Borgo (3), M. Maschietto (4)
This work presents the microfabrication and preliminary characterization of a fully integrated microdevice for in-vitro single cell assays. This technological platform combines IDEs and MEA-based modules for cell addressed delivery of bio-functionalized nano/microparticles and single cell electroporation respectively.
|The Hind Sight of Nanotechnology|
Suman Lahiri, Mamta Shah
Nanotechnology addresses manipulation and study of structures and devices with length scales in the 1- to 100-nanometers range. Market growing upto 1 to 2.95 trillion $ by 2015, projects nanotubes to cross US$5 billion by the year 2012. In view of the toxicity that can be posed by it serious problems can threaten to neutralize the gains of nanotechnology.
|Experimental Determination of ADMET Parameters in High Throughput, Using Colloidal Gold Composites and a Conductive Polymer as Reporting Reagents|
Roberto Martinez-Neira, Patricia De Pril, Anne Van Hoonacker, Patrick Englebienne
We developed and validated a high throughput in vitro setting to experimentally determine hSA and Orosomucoid affinities, fraction absorbed, Log BBB and Redox potential of NCEs using gold nanoparticles functionalized with proteins, lipids or conductive polymers. Such automated ADME assays provide means for more objective decisions in early drug discovery.
|TOWARDS MULTI-ANALYTE BIOSENSOR FOR FOOD SCREENING BASED ON IBIS IMAGING SPR|
S. Rebe Raz , M. Bremer, W. Norde
Our aim is to develop a dedicated tool for multi-analyte detection in food based on IBIS Surface Plasmon Resonance imaging platform using a microarray format. Utilizing a multi-immunoassay on top of the SPR- imaging platform will allow rapid and simultaneous detection of various food ingredients and contaminants (e.g. food allergens, residues of veterinary drugs and environmental contaminants), providing the end user with a detailed food profile.
|Combination of a transient assay based ChIP technology and transcriptome analyses for the exploration of potential transcription factor binding sites|
Shu-Jen Chou (presenter), Li-Teh Chen, Yi-Hang Li, Chiung-swey Joanne Chang and Shu-Hsing Wu
1. Combination of protoplast transient expression system and Nickel resin based purification is an useful approach for chromatin enrichment that can be used to identify transcription factor’s potential target genes. 2.Comparison our ChIP-on-chip target genes with in vivo binding targets using two whole genome tiling array platforms showing high degree of overlap between two methods.