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Design of an innovative microfluidic system to study chemotactic transmembranal migration of leukocytes
Elena Bianchi (a)(b)(c), Elwin Vrouwe (b) , Laganà Katia (a) , Margherita Cioffi (a), Marko Blom (b), Bob Lansdorp (b), Gabriele Dubini (a)

Aim of this project is to develop a versatile and highthroughput microdevice, to be employed in studies of leukocytic chemotaxis, shear stress dependent.

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Perfecting Bacterial Tumor Treatment using Microfluidic Bioreactors
Bhushan J. Toley, Brett M. Babin, Colin L. Walsh, Neil S. Forbes

Engineered bacteria provide a great opportunity to overcome the limitations of current cancer chemotherapeutics. We have developed microfluidic continuous flow-through devices as in-vitro models of tumor tissue and used them to quantify therapeutic efficacies of bacterial strains.

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A PDMS Sample Pre-treatment Device for the Optimization of Electrokinetic Manipulations of Serum
Tim Abram, Dr. David Clague

A PDMS “sample pretreatment” device has been fabricated in order to selectively tune key biological sample parameters which will optimize the sample for subsequent electrokinetic manipulations. We have shown that a raw sample can be homogeneously combined with specific buffers in a DC pulse micromixer in under 1.5 seconds.

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A novel dynamic biochip platform for real-time detection and quantification of proteins
M. Rendl, T. Brandstetter, J. Rühe

A proof of principle of a protein biochip platform permitting analysis of multiple clinically relevant proteins is presented.

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HIV POC testing by ssDNA coupled with NALF
Natasha Gous, Lesley E. Scott, Alexio Capovilla, Natela Rekhviasvili, Wendy Stevens

A isothermal amplification termed Reverse Transcription Loop Dependant Amplification (RT-LDA) was developed with an affordable nucleic acid lateral flow detection (NALF) system, as one component of a potential POC HIV-1 RNA assay for subtypeC. RT-LDA makes use of a primer design that efficiently converts viral RNA into ssDNA amplicons, in 1 hour at 53ºC. Due to the single stranded nature of the product, the amplicon could be detected using NALF.

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Microfluidic assembly of magnetic gel particles
C. H. Chen, A. R. Abate, D. Lee, E. M. Terentjev and D. A. Weitz

Monodisperse spherical magnetic gel particles containing asymmetric infrastructure were fabricated by a new microfluidics-based technique using double-emulsion droplet as templates. Double emulsions with functional cores and hydrogel shells were generated by the flow-focusing drop makers with special wettability patterning. Particles were made with a consistently anisotropic internal structure, which leads to their uniform anisotropy to perform the highly rotational controls by applying the magn

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Microfluidic PCR device for diagnostic pathogen detection
Johannes R. Peham, Hannes Steiner, Walter Grienauer, Rudolf Heer, Michael J. Vellekoop, Christa Nöhammer, Herbert Wiesinger

In this work a microfluidic cyclic flow PCR device is presented, which is capable of replicating the bacterial genomic DNA sequence of the 16S ribosomal RNA. The standard laboratory processing time of 3 h could be decreased to 60 min with the microfluidic reactor without loosing PCR efficiency. Integrating an optical fluorescence detector for dsDNA measurement would evolve this device into a micro total analysis system.

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Design and Fabrication of a Micro PCR Module for POC Applications
E. Morganti, C. Collini, C. Ress, A. Adami, L. Lorenzelli

The design and fabrication process of a micro PCR module is presented. The final system will be integrated in an innovative Lab on a Chip (LOC) to provide a technological platform able to detect autoimmune genetic diseases.

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Fabrication and characterization of a fully integrated microdevice for in-vitro single cell assays
C. Collini, E. Morganti, R. Cunaccia, L. Odorizzi, C. Ress, and L. Lorenzelli (1), A. De Toni, G. Marinaro (2), M. Borgo (3), M. Maschietto (4)

This work presents the microfabrication and preliminary characterization of a fully integrated microdevice for in-vitro single cell assays. This technological platform combines IDEs and MEA-based modules for cell addressed delivery of bio-functionalized nano/microparticles and single cell electroporation respectively.

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Showing Results 71 - 80 of 148
Scientific News
Mechanically Stimulating Stem Cells
MIT biological engineering graduate student Frances Liu is studying ways to alter mechanical properties of cell environments to produce desired chemical outputs.
Catching And Releasing Tiny Molecules
New technique for sorting biomolecules could lead to efficient clinical diagnostics and chemical purification.
A Single-Cell Breakthrough
UNC School of Medicine scientist Scott Magness and collaborators use their newly developed technology to dissect properties of single stem cells. The advancement will allow researchers to study gastrointestinal disorders and cancers like never before.
Tiny Optical Antennas: A Nano Revolution
Newly developed tiny optical antennas, which function like spotlights at the nanoscale, offer the potential to measure food safety, identify air pollutants and even quickly diagnose and treat deadly diseases, like cancer.
Solving The Mystery Of The Dancing Droplets
Years of research satisfy a graduate student's curiosity about the molecular minuet he observed among drops of ordinary food coloring.
Hearts On A Chip To Aid Drug Screening
UC Berkeley bioengineers have developed a heart-on-a-chip which can be used for drug safety screening.
Lab-On-Paper Invented
New paper-based platform for conducting a wide range of complex medical diagnostics.
Single-Cell, 42-plexed Protein Analysis Achieved with a New Microchip Technology
A novel microdevice capable of detecting 42 unique immune effector proteins has been developed.
Lab-on-a-Chip to Study Single Cells
Scientists at EPFL have developed a new lab-on-a-chip technique to analyze single cells from entire population. The new method, which uses beads and microfluidics can change the way we study mixed populations of cells, such as those of tumors.
Mini Synthetic Organism Instead Of Test Animals
Using a compact multi-organ chip, and those of three separate microcircuits, researchers can study the regeneration of certain kidney cells.
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