|In-plane detection of fluorescence signals in microfludic lab-on-chip flow cytyometry|
James Hoyland, Casper Kunstmann-Olsen
Several means of extracting fluorescence signals from flowing cell suspensions in a single plane are examined. Simple microfluidic flow cytometer structures incorporating lateral hydrodynamic focusing were molded in PDMS. Several geometries for embedding optical fibers and custom molded waveguides into the same structure were compared. Improvement in light yield is examined with molded cylindrical lenses and by using channels filled with high refractive index polymers as waveguides.
|Gene List Significance Index (GLSI) improves our method High Performance Chip Data Analysis dramatically - Quantifying the quality of different lists of analyzed significant genes|
Joachim R. Grün (1), Andreas Grützkau (1), Marta Steinbrich-Zöllner (3) Thomas Häupl (2), Ria Baumgrass (1), Jochen Sieper (3), Gerd-Rüdiger Burmester (2), Andreas Radbruch (1)
Our gene expression profiling strategy High Performance Chip Data Analysis (HPCDA) was improved with a method for quantifying the quality of different gene lists (GLs) with the new Gene List Significance Index (GLSI). With GLSI it is possible to decide which of two different extracted GLs has highest fraction of true positives, of high fold change or low p-value genes. With GLSI we could empirically optimize HPCDA-Score for ranking genes.
|PROTEOME WIDE PLASMA PROFILING USING ANTIBODY SUSPENSION BEAD ARRAYS|
Maja Neiman, Ulrika Igel, Burcu Ayoglu, Kimi Drobin, Mathias Uhlén, Peter Nilsson and Jochen M. Schwenk
A newly developed antibody suspension bead array assay allows for a systematic and high-throughput plasma profiling. This microtiter based assay uses antibody-coupled beads for a multiplexed analysis of minute amounts of directly labelled samples. The key requirement of a?nity reagents towards all human proteins is met by the Human Protein Atlas project.
|Evaluation of microfluidic digital PCR for the detection of cancer biomarkers|
Rebecca Sanders, Claire Bushell, Carole Foy, Daniel J. Scott
dPCR is achieved by sample partitioning prior to PCR amplification such that each reaction chamber contains one copy or less of target DNA. This dilution becomes the limiting factor and an accurate target molecule count is achievable. This study evaluates dPCR’s quantitative capabilities and investigates parameters influencing copy number quantification, using the Fluidigm Biomark instrument. Biomark technology combines dPCR theory with a microfluidics platform.
|Identification of differentially expressed transcripts associated to apomixis in B r a c h ia r ia using cDNA microarrays|
Eduardo Gorrón 1 , 2, Diana Bernal 1, Silvia Restrepo & Joe Tohme
Apomixis is a trait which allows flowering plants to produce seeds by asexual ways. Molecular mechanisms behind this phenomenon are poorly understood. We used cDNA microrrays coupled to substractive libraries to find genes related to apomixis in Brachiaria. Genes related to meiosis and cell division, and some putative transcription factors, were overexpressed in sexual plants. It may indicate that apomixis could be caused by downregulation of these genes.
|Design of an innovative microfluidic system to study chemotactic transmembranal migration of leukocytes |
Elena Bianchi (a)(b)(c), Elwin Vrouwe (b) , Laganà Katia (a) , Margherita Cioffi (a), Marko Blom (b), Bob Lansdorp (b), Gabriele Dubini (a)
Aim of this project is to develop a versatile and highthroughput microdevice, to be employed in studies of leukocytic chemotaxis, shear stress dependent.
|Perfecting Bacterial Tumor Treatment using Microfluidic Bioreactors|
Bhushan J. Toley, Brett M. Babin, Colin L. Walsh, Neil S. Forbes
Engineered bacteria provide a great opportunity to overcome the limitations of current cancer chemotherapeutics. We have developed microfluidic continuous flow-through devices as in-vitro models of tumor tissue and used them to quantify therapeutic efficacies of bacterial strains.
|A PDMS Sample Pre-treatment Device for the Optimization of Electrokinetic Manipulations of Serum|
Tim Abram, Dr. David Clague
A PDMS “sample pretreatment” device has been fabricated in order to selectively tune key biological sample parameters which will optimize the sample for subsequent electrokinetic manipulations. We have shown that a raw sample can be homogeneously combined with specific buffers in a DC pulse micromixer in under 1.5 seconds.
|A novel dynamic biochip platform for real-time detection and quantification of proteins|
M. Rendl, T. Brandstetter, J. Rühe
A proof of principle of a protein biochip platform permitting analysis of multiple clinically relevant proteins is presented.