A Valve-Controlled Microfluidic Sysytem for Rapid Two-Dimensional Electrophoresis

Overview

(1) Rapid (350 s) two-dimensional electrophoretic separation of proteins in a PDMS microfluidic chip

(2) Valves located at the intersection allow the simultaneous sample transfer between the 1-D single channel and 2-D channel array

(3) Scanning confocal method extends the size of both 1-D channel and 2-D array thus providing higher peak capacity for practical application

(4) Combining with the silicon electrospray tip facilitates the connection of electrophoresis and mass spectrometry.