Corporate Banner
Satellite Banner
Technology Networks Header
Thursday, October 02, 2014
Technology Networks
 
Register | Sign in
Home Page > Videos > Mapping Chemical Gradients in Living Tissue in Space and Time Using Microfluidics
  Videos

Return

Mapping Chemical Gradients in Living Tissue in Space and Time Using Microfluidics
SelectBio

Chemical gradients drive many processes in biology, ranging from nerve signal transduction to ovulation. At present, microscopy is the primary tool used to understand these gradients. Microscopy has provided many important breakthroughs in our understanding of the fundamental biology, but is limited due to the need to incorporate fluorescent molecules into biological systems. As a result, there is a need to develop tools that can measure chemical gradient formation in biological systems that do not require fluorescent modification of the targets in question, can be multiplexed to measure more than one molecule and is compatible with a variety of biological sample types, including in vitro cell cultures and ex vivo tissue slices. Work from our group on the development of microfluidic tools to measure chemical gradients in living tissue will be presented. Two separate systems are under development. The first is a microfluidic system designed to analyze metabolite and protein expression from tissue. The sampling system can resolve up to 19 different ports and can be interface with either electrochemical or fluorescence-based detection methods. Using these two detection methods, we are capable of analyzing the release of either small molecule metabolites or proteins and peptides using immunoassays. The second system uses a high-density electrode array to image release of electrochemically active metabolites like nitric oxide from live tissue slices. Electrochemical characterization of this system combined with a microfluidic system for gradient generation will be shown.

Request more information
Company product page



For access to this article, enter your email address to instantly recieve a Password Reset link.

Please enter your email address below:

Existing users please Sign In here. Don't have an account? Register Here for free access.

Don't have an account? | Register Here

Scientific News
UW-Madison Team Developing ‘Tissue Chip’ to Screen Neurological Toxins
A faster, more affordable way to screen for neural toxins.
Modular Components Make Building 3-D “Labs-on-a-Chip” a Snap
New building blocks take microfluidics from flat to 3-D quickly and easily.
NIH Funds Next Phase of Tissue Chip for Drug Screening Program
Scientists will integrate chips mimicking human organ functions into full body system to evaluate drugs.
Airway Muscle-On-A-Chip Mimics Asthma
Tissue-level model of human airway musculature could pave way for patient-specific asthma treatments.
New Chip Promising For Tumor-Targeting Research
The new system, called a tumor-microenvironment-on-chip device, will allow researchers to study the complex environment surrounding tumors and the barriers that prevent the targeted delivery of therapeutic agents.
Gene Expression Patterns in Pancreatic CTCs Revealed
Distinct patterns of gene expression in several groups of CTCs were identified, including significant differences from the primary tumor that may contribute to the ability to generate metastases and prove to be targets for improved treatment of the deadly tumor.
Blood Cleanser for Sepsis
Dialysis-like ‘biospleen’ quickly filters bacteria, fungi and toxins.
Stanford Engineers Aim to Connect the World with Ant-sized Radios
Costing just pennies to make, tiny radios-on-a-chip are designed to serve as controllers or sensors for the 'Internet of Things.'
Lab on a Breathing Chip
Human nasal epithelial cells, cultured on a microchip, react to air pollutants just like they would in the upper airway.
Micropumps for Lab-on-a-Chip Disease Diagnosis
Reliable, inexpensive, programmable pumps could help make the diagnosis of many global life-threatening diseases easy and affordable.
Scroll Up
Scroll Down
Skyscraper Banner
Skyscraper Banner
Follow TechNetcom1 on Twitter
Technology Networks Ltd. on LinkedIn
Go to LabTube.tv
Go to Lab-on-a-Chip RSS Feed