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 | Chemical Biology Approaches to Target Protein Modification in Disease Megan Wright, Imperial College London, speaking at Discovery Chemistry Congress 2013. |
 | Guiding Treatment Decisions in Cancer using Protein Biomarker Measurements: from Design to Utility Prof. Stephen Pennington, University College Dublin, speaking at Discovery Chemistry Congress 2013. |
Mass-Spec Based Screening Assay Trends 2013
HTStec
This market report summarizes the results of HTStec's 1st industry-wide global web-based benchmarking survey on mass spectrometry (MS)-based screening assays carried out in February 2013.
Lab Instrument Rental Trends 2012
HTStec
This market report summarizes the results of HTStec’s industry-wide global web-based benchmarking survey on lab instrument rental carried out in September 2012.
Single Cell Technologies Trends
HTStec
This market report summarizes the results of HTStec’s industry-wide global web-based benchmarking survey on single cell technologies carried out in June 2012.
Life Science and Chemical Instrumentation Market (2011 - 2016)
MarketsandMarkets
Spectroscopy, Chromatography, DNA Sequencer & Amplifier, Lab Automation, Array, Flow Cytometer, Electrophoresis, Immuno Assay & Others
Biomarkers in Late-Stage Clinical Trials: Applications, opportunities and activities of leading players
Business Insights
Biomarkers reduce the attrition rate of late-stage clinical trials by assessing drug potential in terms of efficacy and toxicity at the early stages of clinical development. Thus, biomarkers significantly increase productivity, lower the cost and duration of clinical trials, and help researchers complete the drug development process at a faster pace.
Top 10 Drug Discovery Technologies - Strategic Analysis and Global Forecasts
MarketsandMarkets
The top 10 drug discovery technologies play a vital role in the growth of the entire pharmaceutical industry as they are the key contributors in the swift introduction of blockbusters and innovative drugs. The applications of these technologies in the introduction of drugs have created significant opportunities for market growth. The slow introduction of new blockbuster drugs is a major concern which the drug development companies are catering to by using these top technologies.
Multiplexed Diagnostics 2010
Select Biosciences Ltd
The multiplexed diagnostics market has grown rapidly and generated sales of approximately $2.4 billon in 2009, but is now balanced on multiple tipping points.
Genomics, Proteomics and Metabolomics in Diagnostics: Market landscape, innovative technologies and future outlook
Business Insights
There has been a significant transition in the diagnostic techniques created with increasing technological innovations. In the future, the focus will be on genetic study and disease predisposition, which will test patients' probability of acquiring a disease or a disorder, enabling precautionary steps.
HTStec
This market report summarizes the results of HTStec's 1st industry-wide global web-based benchmarking survey on mass spectrometry (MS)-based screening assays carried out in February 2013.
Lab Instrument Rental Trends 2012
HTStec
This market report summarizes the results of HTStec’s industry-wide global web-based benchmarking survey on lab instrument rental carried out in September 2012.
Single Cell Technologies Trends
HTStec
This market report summarizes the results of HTStec’s industry-wide global web-based benchmarking survey on single cell technologies carried out in June 2012.
Life Science and Chemical Instrumentation Market (2011 - 2016)
MarketsandMarkets
Spectroscopy, Chromatography, DNA Sequencer & Amplifier, Lab Automation, Array, Flow Cytometer, Electrophoresis, Immuno Assay & Others
Biomarkers in Late-Stage Clinical Trials: Applications, opportunities and activities of leading players
Business Insights
Biomarkers reduce the attrition rate of late-stage clinical trials by assessing drug potential in terms of efficacy and toxicity at the early stages of clinical development. Thus, biomarkers significantly increase productivity, lower the cost and duration of clinical trials, and help researchers complete the drug development process at a faster pace.
Top 10 Drug Discovery Technologies - Strategic Analysis and Global Forecasts
MarketsandMarkets
The top 10 drug discovery technologies play a vital role in the growth of the entire pharmaceutical industry as they are the key contributors in the swift introduction of blockbusters and innovative drugs. The applications of these technologies in the introduction of drugs have created significant opportunities for market growth. The slow introduction of new blockbuster drugs is a major concern which the drug development companies are catering to by using these top technologies.
Multiplexed Diagnostics 2010
Select Biosciences Ltd
The multiplexed diagnostics market has grown rapidly and generated sales of approximately $2.4 billon in 2009, but is now balanced on multiple tipping points.
Genomics, Proteomics and Metabolomics in Diagnostics: Market landscape, innovative technologies and future outlook
Business Insights
There has been a significant transition in the diagnostic techniques created with increasing technological innovations. In the future, the focus will be on genetic study and disease predisposition, which will test patients' probability of acquiring a disease or a disorder, enabling precautionary steps.
Whole Gel Processing Procedure for GeLC-MS/MS Based Proteomics
The procedure will enable clinical proteomics for which GeLC-MS/MS is a popular workflow and sample numbers are relatively high.
Waters Users’ Meeting at ASMS 2013
Register for the Waters Users’ meeting at ASMS 2013 and learn about the latest developments in Mass Spectrometry from Waters experts as we reveal our new technologies and solutions.
Thermo Scientific at ASMS 2013
Learn how scientists and researchers are transforming science by applying innovative solutions to a wide range of qualitative and quantitative applications.
Mass Spectrometry-Based Proteomics in Molecular Diagnostics: Discovery of Cancer Biomarkers Using Tissue Culture
Rapid development of MS-based proteomic techniques in the last decade that has immensely helped researchers in candidate biomarker discovery.
MultiAlign: A Multiple LC-MS Analysis Tool for Targeted Omics Analysis
This article demonstrates how two dissimilar LC-MS feature maps could be analyzed using MultiAlign’s MS/MS traceback and spectral clustering capability.
Simultaneous Determination of Hormonal Residues in Treated Waters using UHPLC-MS/MS
The identification of hormone residues in environment is of special interest because as this is a requirement to take measures in order to regulate and minimize their environmental impact.
Derivatization in Liquid Chromatography for Mass Spectrometric Detection
This review presents an overview of the derivatization reagents in LC/ESI-MS/MS that have been applied to the low molecular weight compounds over the past five years.
Measuring Hordein (Gluten) in Beer - A Comparison of ELISA and Mass Spectrometry
The study suggests that testing using ELISA determination should eventually be replaced with suitable mass spectrometry based methods.
The HUPO Proteomics Standards Initiative- Mass Spectrometry Controlled Vocabulary
In this article, researchers describe the overall structure of the controlled vocabularies, the process by which it has been developed and is maintained and the dependencies on other ontologies.
Development and Characterization of a Novel Plug and Play LC-MS Source
This article reports the development and characterization of a novel, vendor neutral, ultra-high pressure compatible (~10,000 psi) LC-MS source.
The procedure will enable clinical proteomics for which GeLC-MS/MS is a popular workflow and sample numbers are relatively high.
Waters Users’ Meeting at ASMS 2013
Register for the Waters Users’ meeting at ASMS 2013 and learn about the latest developments in Mass Spectrometry from Waters experts as we reveal our new technologies and solutions.
Thermo Scientific at ASMS 2013
Learn how scientists and researchers are transforming science by applying innovative solutions to a wide range of qualitative and quantitative applications.
Mass Spectrometry-Based Proteomics in Molecular Diagnostics: Discovery of Cancer Biomarkers Using Tissue Culture
Rapid development of MS-based proteomic techniques in the last decade that has immensely helped researchers in candidate biomarker discovery.
MultiAlign: A Multiple LC-MS Analysis Tool for Targeted Omics Analysis
This article demonstrates how two dissimilar LC-MS feature maps could be analyzed using MultiAlign’s MS/MS traceback and spectral clustering capability.
Simultaneous Determination of Hormonal Residues in Treated Waters using UHPLC-MS/MS
The identification of hormone residues in environment is of special interest because as this is a requirement to take measures in order to regulate and minimize their environmental impact.
Derivatization in Liquid Chromatography for Mass Spectrometric Detection
This review presents an overview of the derivatization reagents in LC/ESI-MS/MS that have been applied to the low molecular weight compounds over the past five years.
Measuring Hordein (Gluten) in Beer - A Comparison of ELISA and Mass Spectrometry
The study suggests that testing using ELISA determination should eventually be replaced with suitable mass spectrometry based methods.
The HUPO Proteomics Standards Initiative- Mass Spectrometry Controlled Vocabulary
In this article, researchers describe the overall structure of the controlled vocabularies, the process by which it has been developed and is maintained and the dependencies on other ontologies.
Development and Characterization of a Novel Plug and Play LC-MS Source
This article reports the development and characterization of a novel, vendor neutral, ultra-high pressure compatible (~10,000 psi) LC-MS source.
Bruker and SCiLS GmbH Announce an Exclusive Partnership
SCiLS Lab is the software developed by SCiLS GmbH which allows the user-friendly statistical analysis of large MALDI imaging datasets.
Merck Millipore to Highlight LC-MS Capabilities at ASMS
Merck Millipore will highlight their new ZIC®-HILIC technology, a zwitterionic HILIC phase for LC-MS separation of polar compounds.
Phenomenex to Host AACC Workshop on LC/MS/MS for Clinical Diagnostics
Two-day, hands-on program to be held May 15 and 16.
Shimadzu UK Grows Sales Orders by 30%!
Company has achieved sales order growth across many products in the Shimadzu range.
Thermo Fisher Scientific Reports First Quarter 2013 Results
Adjusted earnings per share grew 17% to a record $1.37 with revenue increased 4% to a first quarter record of $3.19 billion.
Waters Reports First Quarter 2013 Financial Results
Waters Corporation reported first quarter 2013 sales of $430 million, an increase of 2% from sales of $420 million in the first quarter of 2012.
Biodesix Announces Agreement with Bruker Daltonics
The collaborative agreement sees Bruker Daltonics support Biodesix’ VeriStrat diagnostic test as well as future diagnostic tests based on MALDI-TOF mass spectrometry.
Bruker and the Genome British Columbia Proteomics Centre to Collaborate
The collaborative effort covers the development and validation of high-throughput assays for determining hemoglobin variants and diabetes risk.
Thermo Fisher Scientific to Acquire Life Technologies Corporation
The definitive agreement will see Thermo Fisher will acquire Life Technologies for $76.00 in cash per fully diluted common share or approximately $13.6 billion.
Microsaic Systems Signs Commercial Agreement
Second major agreement for novel miniaturized chip-based scientific instrument.
SCiLS Lab is the software developed by SCiLS GmbH which allows the user-friendly statistical analysis of large MALDI imaging datasets.
Merck Millipore to Highlight LC-MS Capabilities at ASMS
Merck Millipore will highlight their new ZIC®-HILIC technology, a zwitterionic HILIC phase for LC-MS separation of polar compounds.
Phenomenex to Host AACC Workshop on LC/MS/MS for Clinical Diagnostics
Two-day, hands-on program to be held May 15 and 16.
Shimadzu UK Grows Sales Orders by 30%!
Company has achieved sales order growth across many products in the Shimadzu range.
Thermo Fisher Scientific Reports First Quarter 2013 Results
Adjusted earnings per share grew 17% to a record $1.37 with revenue increased 4% to a first quarter record of $3.19 billion.
Waters Reports First Quarter 2013 Financial Results
Waters Corporation reported first quarter 2013 sales of $430 million, an increase of 2% from sales of $420 million in the first quarter of 2012.
Biodesix Announces Agreement with Bruker Daltonics
The collaborative agreement sees Bruker Daltonics support Biodesix’ VeriStrat diagnostic test as well as future diagnostic tests based on MALDI-TOF mass spectrometry.
Bruker and the Genome British Columbia Proteomics Centre to Collaborate
The collaborative effort covers the development and validation of high-throughput assays for determining hemoglobin variants and diabetes risk.
Thermo Fisher Scientific to Acquire Life Technologies Corporation
The definitive agreement will see Thermo Fisher will acquire Life Technologies for $76.00 in cash per fully diluted common share or approximately $13.6 billion.
Microsaic Systems Signs Commercial Agreement
Second major agreement for novel miniaturized chip-based scientific instrument.
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Processing and Managing Analytical Data to Extract and Share Knowledge – How Far We’ve Come and Why It’s So Hard?
Advanced Chemistry Development Inc.
This paper discusses the ACD/Spectrus Processor, the new all-in-one analytical data processing and chemical characterization software.
UPLC-MS/MS for the Screening, Confirmation and Quantification of 32 Drugs Illegally Added to Herbal/Dietary Supplements for the Enhancement of Male Sexual Performance
Supreme Council of Health, Qatar
This poster outlines development of a novel screening method that is suitable for both the detection of known and unknown ED drugs and analogues. Comprehensive spectral data is collected and automatically compared to a prepared library if known drugs.
Ion Chromatography ICP-Q-MS for the Detection of Arsenic Species in Apple Juice
Thermo Scientific
Interest in the determination of Arsenic species in fruit juices has been triggered by recent media reports in the United States claiming that some apple juices may contain high amounts of Arsenic. Inorganic forms of Arsenic (As3+ and As5+) are highly toxic, whereas the organic forms (e.g. arsenobetaine) are not considered toxic. A robust IC-ICP-MS system was developed and shown to be a highly selective and sensitive technique for determination of trace element species.
Characterizing Small Molecules in Biological Extracts using IntelliXtract Data Processing on High Resolution Accurate Mass Time-of-Flight Data
ACD Labs
To detect small molecules in biological extracts, LC/MS performed using high resolution time of flight with accurate mass analysis is a powerful technology. Together with advanced data processing algorithms, accurate mass and relative isotope abundance measurements, molecular formulae can be determined and compounds identified.
Transforming Analytical Data into Knowledge with Software for Metabolite Studies
ACD Labs
ACD/Labs has been developing specialized software for chemical and pharmaceutical research for over 16 years. Their expertise includes multi-technique, multi-instrument data handling, knowledge management of analytical and separations data, chemical drawing, and prediction software for a range of chemical properties as well as spectra and chromatograms.
The LAB2LAB™Advantage
1Novartis 2TTPLabtech Ltd
Lab2Lab is a novel approach to submitting and transporting samples for analysis across an entire site. Sample tubes are registered and methods selected, an ELN reference is assigned and the sample tube is placed into the “Sender”. The system transports the samples using low pressure compressed air and directs them to the most appropriate analytical instrumentation available. The analytical results are then automatically returned to the originators ELN.
Qualitative Comparison of Wine Process Samples with UHPLC and Ultra-High Resolution TOFMS
Leco Corporation
The evaluation, identification, and quantitation of flavones, antioxidants and related nutrients, biogenic amines, and potential contaminants in wine and other foodstuffs provides valuable information on their nutritional and health values.
Analysis of Pesticide Residues in JonaGold Apples Using QuEChERS Approach and Comprehensive Two-Dimensional Gas Chromatography Time-of-Flight Mass Spectrometry (GCxGC-TOFMS)
LECO Corporation
Screening and quantitation of pesticide residues in fruits and vegetables is of utmost importance for public health and safety concerns. These commodities can contain hundreds-to-thousands of analytes, making them extremely difficult for the screening and accurate quantitation of pesticide residues.
Analysis of Fruit Commodities by GC-TOFMS and GCxGC-TOFMS Using QuEChERS Approach
LECO Corporation
The availability of fruit commodities from a wide variety of sources has increased the need for rapid and accurate screening for pesticides in fruit. Not all fruit commodities are brought to market under the strict guidelines of the United States Department of Agriculture. Determining whether or not the Environmental Protection Agency tolerances for various pesticides have been violated is important in the risk assessment for consumers in the United States.
Characterization of Nutrients and Actives in Herbal Supplements and Nutriceuticals Using UHPLC-Multi-Reflecting Time-of-Flight Mass Spectrometry
LECO Corporation
The qualitative and quantitative analysis of the content of nutritional supplements and alternative medicinal therapies is becoming more important and regulation by the FDA is imminent. High resolution mass spectrometry provides a rapid, selective, and useful mechanism for QC of extracts and finished dosage forms, producing a minable, comprehensive data set.
Advanced Chemistry Development Inc.
This paper discusses the ACD/Spectrus Processor, the new all-in-one analytical data processing and chemical characterization software.
UPLC-MS/MS for the Screening, Confirmation and Quantification of 32 Drugs Illegally Added to Herbal/Dietary Supplements for the Enhancement of Male Sexual Performance
Supreme Council of Health, Qatar
This poster outlines development of a novel screening method that is suitable for both the detection of known and unknown ED drugs and analogues. Comprehensive spectral data is collected and automatically compared to a prepared library if known drugs.
Ion Chromatography ICP-Q-MS for the Detection of Arsenic Species in Apple Juice
Thermo Scientific
Interest in the determination of Arsenic species in fruit juices has been triggered by recent media reports in the United States claiming that some apple juices may contain high amounts of Arsenic. Inorganic forms of Arsenic (As3+ and As5+) are highly toxic, whereas the organic forms (e.g. arsenobetaine) are not considered toxic. A robust IC-ICP-MS system was developed and shown to be a highly selective and sensitive technique for determination of trace element species.
Characterizing Small Molecules in Biological Extracts using IntelliXtract Data Processing on High Resolution Accurate Mass Time-of-Flight Data
ACD Labs
To detect small molecules in biological extracts, LC/MS performed using high resolution time of flight with accurate mass analysis is a powerful technology. Together with advanced data processing algorithms, accurate mass and relative isotope abundance measurements, molecular formulae can be determined and compounds identified.
Transforming Analytical Data into Knowledge with Software for Metabolite Studies
ACD Labs
ACD/Labs has been developing specialized software for chemical and pharmaceutical research for over 16 years. Their expertise includes multi-technique, multi-instrument data handling, knowledge management of analytical and separations data, chemical drawing, and prediction software for a range of chemical properties as well as spectra and chromatograms.
The LAB2LAB™Advantage
1Novartis 2TTPLabtech Ltd
Lab2Lab is a novel approach to submitting and transporting samples for analysis across an entire site. Sample tubes are registered and methods selected, an ELN reference is assigned and the sample tube is placed into the “Sender”. The system transports the samples using low pressure compressed air and directs them to the most appropriate analytical instrumentation available. The analytical results are then automatically returned to the originators ELN.
Qualitative Comparison of Wine Process Samples with UHPLC and Ultra-High Resolution TOFMS
Leco Corporation
The evaluation, identification, and quantitation of flavones, antioxidants and related nutrients, biogenic amines, and potential contaminants in wine and other foodstuffs provides valuable information on their nutritional and health values.
Analysis of Pesticide Residues in JonaGold Apples Using QuEChERS Approach and Comprehensive Two-Dimensional Gas Chromatography Time-of-Flight Mass Spectrometry (GCxGC-TOFMS)
LECO Corporation
Screening and quantitation of pesticide residues in fruits and vegetables is of utmost importance for public health and safety concerns. These commodities can contain hundreds-to-thousands of analytes, making them extremely difficult for the screening and accurate quantitation of pesticide residues.
Analysis of Fruit Commodities by GC-TOFMS and GCxGC-TOFMS Using QuEChERS Approach
LECO Corporation
The availability of fruit commodities from a wide variety of sources has increased the need for rapid and accurate screening for pesticides in fruit. Not all fruit commodities are brought to market under the strict guidelines of the United States Department of Agriculture. Determining whether or not the Environmental Protection Agency tolerances for various pesticides have been violated is important in the risk assessment for consumers in the United States.
Characterization of Nutrients and Actives in Herbal Supplements and Nutriceuticals Using UHPLC-Multi-Reflecting Time-of-Flight Mass Spectrometry
LECO Corporation
The qualitative and quantitative analysis of the content of nutritional supplements and alternative medicinal therapies is becoming more important and regulation by the FDA is imminent. High resolution mass spectrometry provides a rapid, selective, and useful mechanism for QC of extracts and finished dosage forms, producing a minable, comprehensive data set.
Automated Liquid-liquid Extraction of 25-Hydroxy Vitamin D from Serum and Preparation for LC/MC Analysis Using the Hamilton Robotics Microlab STARlet Workstation
Hamilton
This application note demonstrates the automation capabilities of Hamilton Robotics’ Microlab STARlet and how it can be used to prepare clinical serum samples for Vitamin D analysis quickly, reliably and cost effectively.
Visualisation and Comparison of SYNAPT G2-S LC/MS Data with Progenesis LC/MS
Waters Corporation
In this technology brief, we present the precise large scale, label-free analysis of high-density proteomic LC/MS data combined with a qualitative protein strategy that accurately identifies proteins and software that accurately quantifies proteins - both with high reproducibility.
Attamole Detection of Proteins in a Complex Mixture Using the SYNAPT G2-S System
Waters Corporation
Low abundance proteins are often of biological interest and as such, sensitivity and low limits of quantification are key parameters in modern proteomic experiments. The SYNAPT® G2-S System provides improved sensitivity, resulting in the routine detection of attomole levels of tryptically-digested proteins. In this technology brief, we show results from a protein mixture spiked at different concentrations into a cytosolic E.coli lysate.
Data Independent Label-Free Quantification of Low Abundance Proteins
Waters Corporation
The depth to which a complex sample can be interrogated is crucial as this defines the lowest abundance limit of proteins that may be quantified. Waters® SYNAPT® G2-S Mass Spectrometry System has substantial improvements in performance: mass resolution and accuracy, dynamic range, and sensitivity.
Qualitative and Quantitative Characterization of the Metabolome, Lipidome, and Proteome of Human Hepatocytes Stably Transfected with Cytochrome P450 2E1 Using Data Independent LC/MS
Waters Corporation
Drug toxicity is a major reason for the failure of candidate pharmaceuticals during their development. Therefore, it is important to realize the potential for toxicity in a timely fashion. A label-free multi-omics approach has been applied for the analysis of the transfected human hepatocyte cells by implementing LC/HDMSE, providing both qualitative and quantitative information within a single experiment.
MALDI-HDMSE: A Novel ‘Data Independent’ Acquisition Method for the Enhanced Analysis of 2D-Gel Tryptic Peptide Digests
Waters Corporation
Peptide Mass Fingerprinting (PMF) is an unbiased protein identification technique that offers limited sequence information for identification purposes due to the reliance on the m/z measurement of proteolytic peptides. This application note proposes a novel method of acquiring data called High Definition MSE (HDMSE).
TransOmics Informatics Powered by Nonlinear Dynamics
Waters Corporation
This application note introduces the workflow of TransOmics Informatics Software that has been specifically developed for the larger scale analysis of ion mobility MS data from proteomics and metabolomics data sets, comprising technical and biological replicates.
Identification of Human Hemoglobin Protein Variants Using Electrospray Ionization-Electron Transfer Dissociation Mass Spectrometry
Waters Corporation
The global dispersion of hemoglobin variants through population migration has led to the requirement for their identification. An effective mass spectrometry-based procedure involves analysis of the intact globin chains in diluted blood to detect the variant through mass anomalies; this is followed by location of the variant amino acid residue by direct analysis of the enzymatically digested globins without prior chromatographic separation.
Identification of the Site of the Octanoic Acid Post-Translational Modification in Human Ghrelin Using Electron Transfer Dissociation
Waters Corporation
ETD is a powerful fragmentation technique that has proved particularly useful for the determination of labile post-translational modifications of peptides and proteins. The technique has been used to determine the site-specific octanoylation of the peptide Ghrelin which could not be determined using Collision Induced Dissociation.
Automated Online Card Extraction LC/MS System for the Determination of Clozapine and its Metabolites in Rat Blood
Agilent Technologies Inc.
This application note describes the development and validation of a method on the AACE LC/MS system for the online analysis of DBS cards for the quantitation of clozapine and two of its metabolites in rat whole blood.
Hamilton
This application note demonstrates the automation capabilities of Hamilton Robotics’ Microlab STARlet and how it can be used to prepare clinical serum samples for Vitamin D analysis quickly, reliably and cost effectively.
Visualisation and Comparison of SYNAPT G2-S LC/MS Data with Progenesis LC/MS
Waters Corporation
In this technology brief, we present the precise large scale, label-free analysis of high-density proteomic LC/MS data combined with a qualitative protein strategy that accurately identifies proteins and software that accurately quantifies proteins - both with high reproducibility.
Attamole Detection of Proteins in a Complex Mixture Using the SYNAPT G2-S System
Waters Corporation
Low abundance proteins are often of biological interest and as such, sensitivity and low limits of quantification are key parameters in modern proteomic experiments. The SYNAPT® G2-S System provides improved sensitivity, resulting in the routine detection of attomole levels of tryptically-digested proteins. In this technology brief, we show results from a protein mixture spiked at different concentrations into a cytosolic E.coli lysate.
Data Independent Label-Free Quantification of Low Abundance Proteins
Waters Corporation
The depth to which a complex sample can be interrogated is crucial as this defines the lowest abundance limit of proteins that may be quantified. Waters® SYNAPT® G2-S Mass Spectrometry System has substantial improvements in performance: mass resolution and accuracy, dynamic range, and sensitivity.
Qualitative and Quantitative Characterization of the Metabolome, Lipidome, and Proteome of Human Hepatocytes Stably Transfected with Cytochrome P450 2E1 Using Data Independent LC/MS
Waters Corporation
Drug toxicity is a major reason for the failure of candidate pharmaceuticals during their development. Therefore, it is important to realize the potential for toxicity in a timely fashion. A label-free multi-omics approach has been applied for the analysis of the transfected human hepatocyte cells by implementing LC/HDMSE, providing both qualitative and quantitative information within a single experiment.
MALDI-HDMSE: A Novel ‘Data Independent’ Acquisition Method for the Enhanced Analysis of 2D-Gel Tryptic Peptide Digests
Waters Corporation
Peptide Mass Fingerprinting (PMF) is an unbiased protein identification technique that offers limited sequence information for identification purposes due to the reliance on the m/z measurement of proteolytic peptides. This application note proposes a novel method of acquiring data called High Definition MSE (HDMSE).
TransOmics Informatics Powered by Nonlinear Dynamics
Waters Corporation
This application note introduces the workflow of TransOmics Informatics Software that has been specifically developed for the larger scale analysis of ion mobility MS data from proteomics and metabolomics data sets, comprising technical and biological replicates.
Identification of Human Hemoglobin Protein Variants Using Electrospray Ionization-Electron Transfer Dissociation Mass Spectrometry
Waters Corporation
The global dispersion of hemoglobin variants through population migration has led to the requirement for their identification. An effective mass spectrometry-based procedure involves analysis of the intact globin chains in diluted blood to detect the variant through mass anomalies; this is followed by location of the variant amino acid residue by direct analysis of the enzymatically digested globins without prior chromatographic separation.
Identification of the Site of the Octanoic Acid Post-Translational Modification in Human Ghrelin Using Electron Transfer Dissociation
Waters Corporation
ETD is a powerful fragmentation technique that has proved particularly useful for the determination of labile post-translational modifications of peptides and proteins. The technique has been used to determine the site-specific octanoylation of the peptide Ghrelin which could not be determined using Collision Induced Dissociation.
Automated Online Card Extraction LC/MS System for the Determination of Clozapine and its Metabolites in Rat Blood
Agilent Technologies Inc.
This application note describes the development and validation of a method on the AACE LC/MS system for the online analysis of DBS cards for the quantitation of clozapine and two of its metabolites in rat whole blood.
