Corporate Banner
Satellite Banner
Mass Spectrometry
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

Thermo Scientific Announces New UHPLC/MS Method for Trace-Level Analysis of Perfluorinated Compounds in Human Breast Milk

Published: Friday, August 27, 2010
Last Updated: Friday, August 27, 2010
Bookmark and Share
The method utilizes SRM and H-SRM on an integrated UHPLC/MS platform enables robust detection of PFCs at ppt concentrations.

Thermo Fisher Scientific Inc. has developed a new method for sensitive and reproducible quantitation of low levels of perfluorinated compounds (PFCs) in human breast milk. Using selected reaction monitoring (SRM) and highly selective reaction monitoring (H-SRM) on an integrated ultra high performance liquid chromatography mass spectrometry (UHPLC/MS) platform enables reliable and robust detection of PFCs at ppt concentrations in human breast milk.

The new method is detailed in an application note, entitled "Sensitive and Accurate Quantitation of Perfluorinated Compounds in Human Breast Milk using Selected Reaction Monitoring Assays by LC/MS/MS," which is available to download via http://www.thermoscientific.com/pfc.

Liquid chromatography tandem mass spectrometry (LC/MS/MS) has long been the method of choice for the analysis of PFCs, but quantitation accuracy is often jeopardized due to background PFC contamination and matrix interferences. UHPLC/MS is an alternative for monitoring low levels of PFCs in a range of human matrices, delivering precise and reproducible measurements.

Many PFCs are detectable in human serum and breast milk and have been found in the blood of newborns possibly through lactational transfer from mothers. By coupling the Thermo Scientific Accela UHPLC and the PAL autosampler to the Thermo Scientific TSQ Vantage triple-stage quadrupole MS the system was able to provide rapid, accurate and robust analysis and quantitation of six PFCs in human breast milk using SRM and H-SRM.

Elimination of PFC contamination was achieved using a PFC-free Accela pump with a pre-cleaned PFC-free degasser while also replacing Teflon tubing with PEEK tubing. The sensitivity and selectivity afforded by SRM on the TSQ Vantage system obviated the need for any further modifications of the LC configuration, a distinct advantage over other commercial platforms that require the use of in-line contaminant traps or column-switching methods for PFC analysis.

Using the integrated UHPLC/MS platform, separation of the six PFC analytes in a spiked human breast milk matrix was achieved within nine minutes, Thermo Scientific informed. As the majority of interferences from matrices elute early at void volume, elution of the first compound at 3.64 minutes ensured a robust quantitation method. Excellent linearity in detector response was observed while H-SRM eliminated matrix interferences without compromising sensitivity, the company has claimed.

The Accela system, combined with 1.9 µm particle columns, enables efficient chromatographic separations over an expansive range of flow rates and pressures. Delivering up to a tenfold improvement in signal-to-noise ratio compared to existing triple-quadrupole MS systems, the TSQ Vantage facilitates highly sensitive quantitation in matrix-rich samples and enhances analytical precision.


Further Information
Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,500+ scientific posters on ePosters
  • More than 3,700+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Thermo Fisher Q3 Revenues up 31 Percent
Thermo Fisher announced that revenues grew 31% to $4.17 billion for the third quarter ended September 27, 2014.
Thursday, October 23, 2014
Thermo Fisher, NRLs Team to Confirm Dioxins in Feed and Food
Thermo Fisher Scientific worked closely with national reference labs (NRLs) in the European Union to support the development of new regulation.
Monday, September 08, 2014
Thermo Supports Distinguished Analytical Chemistry Seminar Series at NC State
An endowment established by the company provides opportunities to attend presentations by leading analytical chemistry authorities.
Tuesday, August 19, 2014
Reimagining UHPLC
To learn more about the new Vanquish UHPLC system from Thermo Fisher Scientific we spoke to Wulff Niedner, Director of HPLC Product Marketing.
Thursday, July 17, 2014
Thermo Registers San Jose Facility with FDA
The site has established a quality system in accordance with the FDA Quality System Regulations to design and manufacture devices for analyzing samples from patients for in vitro diagnostic use.
Monday, June 16, 2014
Thermo Fisher, BioAnalytix Collaborate
Collaboration to develop advanced biopharmaceutical analysis workflows.
Thursday, June 12, 2014
Thermo, Nuclea Biotechnologies Collaborate to Develop Diabetes Markers
Companies pooling their expertise to develop novel multiplexed research methods for high-throughput quantification of native insulin and its therapeutic analogs.
Monday, June 02, 2014
Thermo, Evans Analytical Group Form Joint Effort to Expand GDMS Applications
Companies team up to apply pulsed glow discharge mass spectrometry to analysis of non-conductive materials, depth profiling.
Thursday, May 15, 2014
Thermo Reports 22% Revenue Rise in Q1 2014
Results include the addition of Life Technologies as of February 4, 2014, and exclude the previously announced related divestitures as of March 22, 2014.
Wednesday, April 23, 2014
Thermo, Harvard Establish Center for Multiplexed Proteomics
Thermo Fisher Scientific Center for Multiplexed Proteomics at Harvard Medical School aims to expedite understanding of complex disease mechanisms.
Thursday, March 20, 2014
Thermo Completes Life Tech Acquisition
The completion of the transaction follows the receipt of all required regulatory approvals.
Tuesday, February 04, 2014
Thermo Reports 6% Q4 Revenue Growth
Thermo Fisher Scientific Inc. reported its financial results for the fourth quarter and full year ended December 31, 2013.
Friday, January 31, 2014
Biosimilar Characterization R&D Facility Opens in Austria
University of Salzburg, Sandoz and Thermo Fisher Scientific collaborate to advance protein-based pharmaceutical development, production, QA/QC.
Wednesday, October 23, 2013
Thermo Fisher Scientific and the DTU Form Systems Biology Collaboration
Orbitrap Fusion Tribrid LC-MS technology to power new cellular signaling network research.
Monday, September 02, 2013
Thermo Fisher Scientific Establishes Pesticide Analysis Center of Excellence
The center helps users apply the latest technology and expertise to challenging pesticide analysis in food and environmental laboratories.
Thursday, August 15, 2013
Scientific News
Structural Discoveries Could Aid in Better Drug Design
Scientists have uncovered the structural details of how some proteins interact to turn two different signals into a single integrated output.
New ACE-inhibiting Molecule Found in the Asparagus
Scientists have determined that sulfur-containing compounds in plants can inhibit ACE.
The Linking of Molecules and Microbes
Researchers have succeeded in making antibiotic substances and their bacterial producers simultaneously visible.
PTR-MS Breath Test Shows Potential for Detecting Liver Disease
Researchers at the University of Birmingham have published results that suggest a non-invasive breath test for liver disease using an IONICON PTR-MS.
New Test Could Distinguish Between Bipolar Disorder, Depression
Scientists are developing a test that could tell the difference between bipolar disorder and depression.
Early Detection of Lung Cancer
The University of Manchester has signed a collaboration agreement with Abcodia to perform proteomics studies on a cohort of non-small cell lung cancer cases from the UKCTOCS biobank, with the aim of discovering new blood-based biomarkers for earlier detection of the disease.
Detecting Hidden Ingredients
Researchers from China have used mass spectrometry to reveal the use of undeclared substances in dietary supplements.
How the Lung Repairs its Wounds
Researchers have gained detailed insights into the dynamic remodeling of the tissue during lung repair.
Automation Abound at AACC in Atlanta
Discover the latest breakthroughs, trends and products from the AACC Annual Meeting & Clinical Lab Expo.
Could a simple saliva test detect Alzheimer's?
Researchers have presented findings suggesting that a simple, non-invasive diagnostic for Alzheimer's could be within reach.
Scroll Up
Scroll Down
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,500+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,700+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FREE!