Corporate Banner
Satellite Banner
Mass Spectrometry
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

AB SCIEX Expands its iChemistry™ Solutions Portfolio with Sensitivity-enhancing Reagents for Mass Spectrometry

Published: Thursday, May 24, 2012
Last Updated: Thursday, May 24, 2012
Bookmark and Share
Amplifex™ reagents support the company’s strategy to push the limits of detection.

AB Sciex Pte. Ltd has announced the commercial availability of Amplifex™ Reagents, the first of a new family of advanced chemistries designed to boost the performance of mass spectrometers, improve data precision and significantly increase sensitivity beyond conventional limits.

The new reagents join the industry-leading iTRAQ® reagents for proteomics and IDQuant™ standards for pesticide analysis in the company’s intelligently-designed portfolio of mass spectrometry-based tagging chemistries, now called SCIEX iChemistry™ Solutions.

Industry research scientists will be presenting their novel work using these reagents at the ASMS conference in Vancouver this week.

Today, scientists around the world are working on assays that require levels of quantitation not possible with even the most sensitive and robust mass spectrometry instruments.

While mass spectrometry is a powerful technology for compound detection and quantitation, sensitivity can sometimes be negatively impacted by matrix suppression, high background noise levels, poor ionization efficiency or poor compound fragmentation.

With the Amplifex™ Reagents, improvements in sensitivity, compared to even the most sensitive of mass spectrometry instruments, can range from 5x to 1000x, depending on the analyte.

Amplifex™ Reagents are the only derivatizing agents custom designed, synthesized and tested to boost ionization efficiency, improve fragmentation and chromatographic properties, and overcome other workflow restraints that laboratories can face, including minimal available sample volumes.

Among the laboratories that had early access to the new Amplifex™ Reagents was the Wisconsin National Primate Research Center & Institute for Clinical & Translational Research at the University of Wisconsin-Madison.

As part of their study into the metabolomics systems of primates, scientists had struggled to achieve the sensitivity levels necessary to examine 1α,25-Dihydroxyvitamin D3.

After incorporating the new Amplifex™ Diene reagent into their workflow however, their sensitivity dilemma was solved.

“Using Amplifex™ Diene, we were able to achieve LOD of 2 pg/ml on the QTRAP® 5500 system for both 1α,25-Dihydroxyvitamin D3 and 1α,25-Dihydroxyvitamin D2, starting with a sample volume of 200 µL, thereby using half the serum volume we would otherwise have used,” said Toni E. Ziegler, Ph.D., Wisconsin National Primate Research Center & Institute of Clinical & Translational Research, University of Wisconsin-Madison. “Through the use of intelligent chemistry, AB SCIEX is pushing the limits of sensitivity to levels unachievable with current LC/MS/MS methods.”

As part of her research into biomarker discovery for Cerebrotendinous Xanthomatosis disease, the new Amplifex™ Keto reagent enabled a scientist at Oregon Health & Science University to successfully measure low concentrations of specific ketosterols, despite the sensitivity and low sample volume requirements necessary for her research.

“The new Amplifex™ Keto reagent is very easy to use and fast, and it enabled me to achieve sensitivity levels of 2.5-5 pg/ml using only 5ml plasma,” said Andrea DeBarber, PhD., Research Assistant Professor, Physiology & Pharmacology Department, Associate Director, Bioanalytical Shared Resource Facility, Oregon Health & Science University. “I agree that the new Amplifex reagent is truly expanding the boundaries of sensitivity for ketosterol biomarker quantification.”

iTRAQ® and the new Amplifex™ Reagents are a part of a family of SCIEX iChemistry™ Solutions, the world’s only reagents and consumables custom designed for mass spectrometry applications.

“We are not only pushing the limits of sensitivity through instrumentation, but we are also pushing the limits through chemistry,” said Babu Purkayastha, Director of R&D for Consumables at AB SCIEX. “We are known for our iTRAQ chemistries, and with our new Amplifex reagents, we are once again enabling researchers to perform challenging analyses and workflows by mass spectrometry, so they can address key analytical and biological questions. Going forward, we will continue to innovate in this area of advanced chemistries.”

Derivatization is no longer a painful, day-long process. With Amplifex™ Reagents, sample preparation workflows are quick, simple, reproducible and robust as well as yielding higher recoveries than traditional reagents. The first two sets of reagents in the Amplifex™ line are:

• Amplifex™-Diene Reagent - a reagent that can be used to derivatize any molecule with a cis-diene system, including compounds such as Vitamin D3 and Vitamin D2 analogs, such as 1α, 25-Dihydroxyvitamin D3 and 1α, 25-Dihydroxyvitamin D2, among others. The result is a signal enhancement of up to 1000-fold.

• Amplifex™-Keto Reagent - a reagent that is universal and reacts with keto or aldehyde compounds, including ketosteroids, such as testosterone, among others. Signal enhancements improve mass limits of detection by up to 500-fold.


Further Information
Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,400+ scientific posters on ePosters
  • More than 3,700+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

SCIEX and New Objective Announce Partnership
New nanospray sources to offer maximum sensitivity and robustness for NanoLC-MS.
Tuesday, June 02, 2015
SCIEX and New Objective Partner
Partnership will provide advanced nanospray ionization technologies for next-generation proteomics.
Tuesday, May 26, 2015
SCIEX and Hepregen Announce Co-Marketing Agreement
Alliance establishes SCIEX and Hepregen as a one-stop solution provider for drug metabolism investigators.
Friday, May 22, 2015
SCIEX and Mass Consortium Corporation Announce Exclusive Reseller Agreement
SCIEX to provide customers with XCMSplus software solution for simplified and accelerated metabolomics workflows.
Tuesday, March 31, 2015
SCIEX and Labor Berlin Collaboration to Advance Forensic Testing
Collaboration for the development of a hybrid Quadrupole Time-of-Flight (TOF) MS/MS reference library.
Thursday, March 05, 2015
New Capabilities for Bioanalysis Studies at the International Pharmaceutical Research Center
AB SCIEX Triple Quad™ 6500 LC/MS/MS systems enable new levels of sensitivity for clinical trials of inhaled drugs.
Wednesday, February 11, 2015
Improved Accuracy of Pesticide Detection in Food
European Reference Laboratory improves accuracy of pesticide detection in food with AB SCIEX mass spectrometry instrumentation.
Wednesday, February 04, 2015
AB SCIEX Wins New Product Innovation Award in Asia Pacific
The award recognizes the company’s continued innovation in the mass spectrometry industry and a number of new products introduced into the market in recent years.
Thursday, October 30, 2014
AB SCIEX Scientist Wins HUPO 2014 Science and Technology Award
Winner becomes second team member to be recognized for the commercialization of transformative chemistries such as iTRAQ® eagents.
Monday, October 06, 2014
AB SCIEX, Illumina Collaborate to Create Multi-omics Cloud-Computing Environment
Integration of SWATH™ Apps with BaseSpace® brings next-generation proteomics and next-generation sequencing together for the first time.
Thursday, October 02, 2014
AB SCIEX, Dalton Pharma Services Announce ADC Partnership
Research collaboration aims to develop Antibody-Drug Conjugate analysis capabilities.
Wednesday, August 27, 2014
Barry Karger Receives Arnold O. Beckman Award
Award honors Prof Karger for his outstanding achievements in the field of electrodriven separation techniques.
Monday, April 28, 2014
AB SCIEX to Present ESI-MS Workshop
The workshop will be presented at HPLC 2014.
Tuesday, April 22, 2014
Halal Meat Authenticity Testing Reassures Consumers of No Pig or Horse Contamination
New LC/MS/MS-based meat speciation method allows food testing laboratories to detect pig and horse contamination in diverse food products, with higher accuracy than existing methods.
Tuesday, January 28, 2014
Researchers Solve a Mystery about Type 2 Diabetes Drug
AB SCIEX TripleTOF® and QTRAP® technologies support breakthrough medical study.
Friday, November 22, 2013
Scientific News
Could a simple saliva test detect Alzheimer's?
Researchers have presented findings suggesting that a simple, non-invasive diagnostic for Alzheimer's could be within reach.
Facebook for the Proteome
Researchers have developed a network for describing protein-protein interactions that can then be used to examine protein interactions that may have biological or clinical significance.
Researchers Reveal Elusive Molecule
A long-standing chemistry puzzle has been solved, with potential implications ranging from industrial processes to atmospheric chemistry.
‘Atomic Detectives’ Scientists that Keep us Safe from Radioactive Criminality
Stephan Richter from the JRC-Institute for Reference Materials and Measurements has examined the merits and limitations of available mass spectrometric instrumentations for nuclear safeguards applications.
MSU Scientists Uncover a ‘Funky Cofactor’
While examining the chemical reactions of bacterial cells in a lab at Michigan State University, researchers found an unusually complex molecule in a protein that completes a simple process.
Aluminum Clusters Shut Down Molecular Fuel Factory
3-D images give clues to extending catalyst life.
Chemists Design a Quantum-Dot Spectrometer
New instrument is small enough to function within a smartphone, enabling portable light analysis.
Creating Bacterial ‘Fight Clubs’ to Discover New Drugs
Creating bacterial “fight clubs” is an effective way to find new drugs from natural sources.
Informatics Tool Helps Scientists Prioritize Protein Modification Research
Researchers have developed a new informatics technology that analyzes existing data repositories of protein modifications and 3D protein structures to help scientists identify and target research on "hotspots" most likely to be important for biological function.
Iron: A Biological Element?
Study shows findings which have meaning for fields as diverse as mining and the search for life in space.
Scroll Up
Scroll Down
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,400+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,700+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FREE!