Corporate Banner
Satellite Banner
Mass Spectrometry
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

Doping Detection Stays a Neck Ahead

Published: Monday, August 13, 2012
Last Updated: Monday, August 13, 2012
Bookmark and Share
Use of LC-MS/MS for the detection of doping in horse racing.

Horse racing is a massive world-wide industry and regular testing is essential to maintain its integrity.

As with human sport, the authorities constantly need to develop methodologies to detect new compounds that drug cheats are using or may start to use. One such compound is peginesatide.

Peginesatide is the first representative of a new class of compounds that mimic the effects of erythropoietin; these include an increase in the number of red blood cells and of haemoglobin levels in the blood.

Both of these increase endurance and so-called erythropoiesis-stimulating agents (ESAs) are banned in human and equine sports.

The approval of the use of peginesatide by the US Food and Drug Administration (FDA) to treat anaemia in patients on dialysis increases its availability and the chance of its use in illegal performance enhancement.

A new mass spectrometry method for detecting peginesatide in humans has already been developed, and now the extension of this for the detection of peginesatide in horse serum is described in an article published in EJMS-European Journal of Mass Spectrometry (doi: 10.1255/ejms.1189) written by Ines Möller, Andreas Thomas, AnkeWingender, Marc Machnik, Wilhelm Schänzer and Mario Thevis from the German Sport University Cologne, Germany.

This is timely since the German Equestrian Federation has recently added peginesatide to its prohibited substance list.

The new method uses electrospray ionization (ESI) liquid chromatography-tandem mass spectrometry (LC-MS/MS). Samples are prepared using the serine endopeptidasesubtilisin to break down the compounds in the sample prior to LC-MS/MS analysis.

According to lead author Mario Thevis, “The method is precise, specific and linear over a wide concentration range. Further, being simple, fast, cost effective, easily transferable to other laboratories and in accordance with the criteria for ‘identification by chromatography and mass spectrometry’ outlined by the Association of Official Racing Chemists (AORC), the method is suitable for routine use in the horse sports drug testing arena.”


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 3,200+ scientific posters on ePosters
  • More than 4,700+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.


Scientific News
ASMS 2016: Targeting Mass Spectrometry Tools for the Masses
The expanding application range of MS in life sciences, food, energy, and health sciences research was highlighted at this year's ASMS meeting in San Antonio, Texas.
Making Metabolite Identification More Efficient
Metasense combines the industry's most-comprehensive metabolic transformation prediction with efficient analysis of LC/MS analytical measurements to identify, visualize, and report chemical biotransformations.
What Makes a Good Scientist?
It’s the journey, not just the destination that counts as a scientist when conducting research.
A New Tool Brings Personalized Medicine Closer
Scientists from EPFL and ETHZ have developed a powerful tool for exploring and determining the inherent biological differences between individuals, which overcomes a major hurdle for personalized medicine.
Rapid Retrieval of Live, Infectious Pathogens from Clinical Samples
An engineered pathogen-binding protein enables rapid isolation of infectious bacteria from joint fluids and accelerates their identification.
Getting a Better Look at How HIV Infects and Takes Over its Host Cells
A new approach, developed by a team of researchers led by The Rockefeller University and The Aaron Diamond AIDS Research Center (ADARC), offers an unprecedented view of how a virus infects and appropriates a host cell, step by step.
Detecting Fake Parmesan Cheeses
Scientists report on a way to catch adulteration of the regional artisanal products.
The Need for Speed
Evaluating MALDI-TOF as a high-throughput screening technology for the pharmaceutical industry.
Process Analysis in Real Time
With a real-time mass spectrometer developed by Fraunhofer researchers, it has become possible for the first time to analyze up to 30 components simultaneously from the gas phase and a liquid, including in-situ analysis.
Triple-Negative Breast Cancer Target Is Found
Researchers at UC Berkeley discover a target that drives cancer metabolism in triple-negative breast cancer.
Scroll Up
Scroll Down
Skyscraper Banner

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,200+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,700+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!