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New Method Speeds and Improves Glycoprotein/Glycopeptide Isolation

Published: Wednesday, August 29, 2012
Last Updated: Wednesday, August 29, 2012
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Concanavalin A column for analysis of glycoproteins and their tryptic glycopeptides.

Thermo Fisher Scientific has announced a new method that can automatically extract glycoproteins from samples, and glycopeptides from tryptic digests, thus enabling faster isolation, better sample recovery, high peak efficiency, and high-throughput capability.

Application Note (AN) 1014: Concanavalin A Column for Analysis of Glycoproteins and Their Tryptic Glycopeptides demonstrates that this approach enhances efficiency by leveraging innovative products.

For example, this new method uses a high-performance liquid chromatography (HPLC)-compatible Concanavalin A (Con A) column that is built on a monolithic support and offers advantages over other Con A columns.

AN 1014 also shows that a fully biocompatible analytical LC system can automate the entire off-line process, from sample enrichment and fraction collection to the reanalysis of collected sample by peptide mapping with both ultraviolet and mass spectrometry detections.

Monitoring oxonium ions with a single quadrupole mass spectrometer is a selective, sensitive, and reliable method that confirms the identity of the glycopeptides captured by the Con A column.

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