Thermo Fisher Scientific Inc., has introduced a new data-independent acquisition (DIA) proteomics capability for the Thermo Scientific Q Exactive high performance quadrupole-Orbitrap LC-MS/MS. The new DIA feature allows the Q ExactiveTM mass spectrometer to collect MS/MS spectra on virtually all ions in a sample by selecting broad m/z windows and fragmenting all precursors in that window. This allows the instrument to quantify nearly all detected peptides in the sample in a single run.
Unlike other DIA-capable mass spectrometry platforms, which require switching to a triple quadrupole instrument to perform targeted peptide quantitation, Q Exactive LC/MS now lets users perform both DIA and targeted quantification experiments on the same instrument.
The DIA data collected on the Q Exactive MS can be processed with Thermo Scientific Pinpoint software 1.3. The DIA acquisition strategy adds another multiplexing method to the Q Exactive instrument, which already allows users to multiplex up to 10 precursor ions for quantification in full MS and MS/MS mode.
The Q Exactive MS offers ultrahigh resolution up to 140,000 This delivers very accurate quantitation by decreasing interference from targeted peptidesThis makes the technique especially useful for quantifying proteins/peptides of interest from DIA experiments. Plus, the Q Exactive instrument provides truly SRM-equivalent quantitative performance.
“The next generation of high-throughput quantitative proteomics is imminent,” said Andreas Huhmer, marketing director, Thermo Fisher. “Multiplexing strategies, such as the data-independent acquisition methods, will play an important role in elevating quantitative proteomics to this new level, and the unique architecture of hybrid Orbitrap instruments will play an important role in advancing the technique.”
The new instrument control software is available as a developer’s kit for Q Exactive LC/MS customers who want to acquire DIA capability.
The Q Exactive instrument is the first commercially available mass spectrometer to combine quadrupole precursor selection with HR/AM Orbitrap mass analysis to deliver high confidence quantitative/qualitative workflows. Thermo Fisher coined the term “Quanfirmation” to describe the instrument’s ability to perform compound identification, quantitation and confirmation in a single run.