Corporate Banner
Satellite Banner
Mass Spectrometry
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

AB SCIEX Advances Proteomics Research with New Eksigent nanoLC System

Published: Monday, September 10, 2012
Last Updated: Monday, September 10, 2012
Bookmark and Share
Industry’s first liquid chromatography instrument to provide plug-and-play interchangeability between nano LC and micro LC on the same platform for discovery proteomics and peptide quantitation.

AB SCIEX announces the launch of the Eksigent ekspert™ nanoLC 400 system.  The new Eksigent system is the world’s first plug-and-play liquid chromatography system that integrates nano LC and micro LC on the same platform, providing the broadest functionality with the benefits of both flow rate ranges.  This flexibility enables fast and easy switching between high-sensitivity discovery proteomics and high-throughput targeted peptide quantitation commonly used in advanced biological studies.  The ekspert nanoLC 400 system is on display at the HUPO conference in Boston this week.  

Discovery proteomics is a strategy in which an LC system in combination with a mass spectrometer is used to identify and quantify proteins in a global untargeted manner.  Once a set of interesting proteins has been identified, targeted techniques are used to verify and validate the results in further sample types by targeted peptide quantitation.  

The ultimate goal is to advance the understanding of systems biology by identifying new biomarkers or new proteins of interest and to potentially improve medical methodologies.  The new Eksigent ekspert nanoLC 400 system improves the efficiency of these proteomics workflows by providing the highest separation quality and reliability.  

The novel, interchangeable LC flow modules in the nanoLC 400 system allow scientists to quickly go from low flow nano LC to a higher flow rate of micro LC, with a simple switch of a module. This is ideal for mixing non-targeted and targeted approaches, especially when used with a next-generation mass spectrometer like the AB SCIEX TripleTOF® 5600+ system. In addition, the retention time stability afforded by advances in microfluidic flow control (MFCPlus™ technology) is critical for quantitative applications such as Scheduled MRMHR Workflow and SWATH™ Acquisition.

“Scientists want to do more with less,” said Remco van Soest, Product Manager for the ekspert nanoLC 400 system at AB SCIEX.  “Because the new ekspert nanoLC 400 system bridges the gap between low flow and higher flow rates in LC/MS workflows, laboratories no longer need separate LC systems to go from discovery to verification and validation in the protein biomarker research continuum.  The plug-and-play functionality of the nanoLC system opens up new possibilities for biomedical and proteomics researchers.”

The nanoLC 400 system is the only low-flow system that – when combined with the existing Eksigent cHiPLC® system – enables highly reproducible proteomics across a broad range of workflows in busy multi-user, multi-instrument laboratories.  The cHiPLC system is a chip-based platform that serves as a "docking station" for easy switching between different types of experiments, such as direct injection and trap-loading. With the new nanoLC 400 system, injections can now be done 5x to 10x faster with a higher precision autosampler for nano LC.  

“The Eksigent nanoLC system enables me to go to a higher flow rate within the same system, when I need it, and that is a tremendous benefit,” said Nick Morrice, Group Leader at the Beatston Institute for Cancer Research in Glasgow, Scotland.  “Nano LC with 75 um ID columns is great for thorough, detailed discovery, but it takes longer than I would like.  Using microflow LC on my TripleTOF 5600 system with the 25 um ID hybrid electrode and the DuoSpray Source, my routine protein identifications using the same injection volume are three times faster than nano LC.  The flexibility of being able to switch between nano LC and micro LC in minutes is really valuable.  It’s a step forward for the proteomics community.”

Quantitative proteomics research continues to evolve, with a continued shift toward targeted analysis and more recently data-independent analysis.  This shift requires a new level of reproducibility and integration for qualitative and quantitative analysis.  The Eksigent ekspert nanoLC 400 system is designed for next-generation proteomics and data-independent workflows, such as MS/MSALL with SWATH™ Acquisition.  Released earlier this year, SWATH Acquisition is a groundbreaking mass spectrometry-based technique that enables quantification of all detectable peptides and proteins in a sample from a single analysis.  

For more information about the Eksigent ekspert nanoLC 400 system, go to www.eksigent.com.


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 3,000+ scientific posters on ePosters
  • More than 4,400+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

SCIEX, QPS Holdings Collaborate
Agreement aims to advance the hybrid LBA-LCMS workflow for biologics quantification.
Wednesday, April 06, 2016
SCIEX, Children's Medical Research Institute Collaborate
SCIEX industrialized proteomics solutions, using SWATH® Acquisition-based workflows and powered by the OneOmics™ Project, will enable large scale proteome studies to advance cancer research.
Thursday, February 11, 2016
SCIEX, A*STAR’s BTI Launch Collaboration
Agreement aims to boost innovation of protein drugs that target new diseases and are safer for patients.
Wednesday, January 27, 2016
SCIEX, Pressure BioSciences Partner
SCIEX and Pressure BioSciences to offer complete solution for next-generation proteomics, to increase the reproducibility and depth of proteomic analysis.
Wednesday, January 13, 2016
SCIEX, University of Manchester Partner
SWATH® Acquisition-based next-generation proteomics technology promises to make industrial proteomics a reality.
Thursday, October 29, 2015
SCIEX Partners with the Paulovich Lab at Fred Hutchinson Cancer Research Center
Collaboration targets research reproducibility crisis with highly validated multiplex quantitation assays.
Wednesday, September 23, 2015
SCIEX and New Objective Announce Partnership
New nanospray sources to offer maximum sensitivity and robustness for NanoLC-MS.
Tuesday, June 02, 2015
SCIEX and New Objective Partner
Partnership will provide advanced nanospray ionization technologies for next-generation proteomics.
Tuesday, May 26, 2015
SCIEX and Hepregen Announce Co-Marketing Agreement
Alliance establishes SCIEX and Hepregen as a one-stop solution provider for drug metabolism investigators.
Friday, May 22, 2015
SCIEX and Mass Consortium Corporation Announce Exclusive Reseller Agreement
SCIEX to provide customers with XCMSplus software solution for simplified and accelerated metabolomics workflows.
Tuesday, March 31, 2015
SCIEX and Labor Berlin Collaboration to Advance Forensic Testing
Collaboration for the development of a hybrid Quadrupole Time-of-Flight (TOF) MS/MS reference library.
Thursday, March 05, 2015
New Capabilities for Bioanalysis Studies at the International Pharmaceutical Research Center
AB SCIEX Triple Quad™ 6500 LC/MS/MS systems enable new levels of sensitivity for clinical trials of inhaled drugs.
Wednesday, February 11, 2015
Improved Accuracy of Pesticide Detection in Food
European Reference Laboratory improves accuracy of pesticide detection in food with AB SCIEX mass spectrometry instrumentation.
Wednesday, February 04, 2015
AB SCIEX Wins New Product Innovation Award in Asia Pacific
The award recognizes the company’s continued innovation in the mass spectrometry industry and a number of new products introduced into the market in recent years.
Thursday, October 30, 2014
AB SCIEX Scientist Wins HUPO 2014 Science and Technology Award
Winner becomes second team member to be recognized for the commercialization of transformative chemistries such as iTRAQ® eagents.
Monday, October 06, 2014
Scientific News
Altered Metabolism of Four Compounds Drives Glioblastoma Growth
Findings suggest new ways to treat the malignancy, slow its progression and reveal its extent more precisely.
Coding and Computers Help Spot Methane, Explosives
Coded apertures improve and shrink mass spectrometers for field use.
Why Bearcats Smell Like Buttered Popcorn
Researchers pinpoint chemical compound that gives rare animal its popcorn-like scent.
UCSD Scientists Receive NIH Grant to Combat Antibiotic Resistance
Interdisciplinary program will use systems biology approaches to understand how antibiotics work in concert with patient’s immune system.
Virus Causing Tilapia Die-Offs Identified
Discovery of the virus causing Tilapia die-offs in Israel and Ecuador points the way to protecting a fish that feeds multitudes.
Novel Collagen Fingerprinting Identifies A Neanderthal
Study from the universities of Oxford and Manchester uses ZooMS technique to identify traces of an extinct human.
Hope for Combating Muscular Dystrophy
Decoding a sugar molecule and identifying a mechanism linking it to MS could help in the development of therapy for the disease.
Decoding Sugar Molecules Offers New Key For Combating Muscular Dystrophy
Japanese scientists find a rare sugar unit called ribitol 5-phosphate within the sugar molecules on the surface of muscle cells. Mutations in 3 genes linked to muscular dystrophy affect the creation of this sugar molecule.
A Vision for Precision Medicine
The University of Manchester and the University of Dundee partner to share disease screening data.
New Targets for Diabetes, Inflammation Discovered
The Scripps Research Institute and Salk Scientists discover 'outlier' enzymes that could offer new targets to treat diabetes and inflammation.
Scroll Up
Scroll Down
SELECTBIO

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,000+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,400+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!