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Clinical Utility of IGF 1 and 2; Determination by High Resolution Mass Spectrometry

Published: Monday, September 24, 2012
Last Updated: Monday, September 24, 2012
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Study demonstrates that accurate mass LC-MS approaches for proteins can offer precise quantitative measurements that meet the criteria for clinical utility.

Abstract

Measurement of insulin-like growth factor-1 (IGF-I) has utility for the diagnosis and management of growth disorders, but inter-assay comparison of results has been complicated by a multitude of reference standards, antibodies, detection methods, and pre-analytical preparation strategies. We developed a quantitative LC-MS method for intact IGF-I, which has advantages in throughput and complexity when compared to mass spectrometric approaches that rely on stable isotope dilution analysis of tryptic peptides. Since the method makes use of full-scan data, the assay was easily extended to provide quantitative measurement of IGF-II using the same assay protocol. The validated LC-MS assay for IGF-I and IGF-II provides accurate results across the pediatric and adult reference range and is suitable for clinical use.

This article is published online in PLoS One and is free to access.


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