Biognosys AG announced the launch of a free academic version of its Spectronaut™ software for analysis of Hyper Reaction Monitoring (HRM) data. HRM is a targeted proteomics technology invented at Biognosys that enables reproducible and accurate quantification of 1000s of proteins in a single instrument run.
HRM is based on data independent SWATH™ Acquisition performed on the AB SCIEX TripleTOF® 5600+ System. SWATH™ Acquisition generates vast and highly convoluted data sets that contain a full representation of all peptide ions present in the sample. The main limitation so far has been the lack of efficient software that could exploit the full potential of this technology for highly multiplexed and reproducible protein quantification.
Spectronaut™ from Biognosys uses a proprietary high speed data format that allows processing of 10’000s transitions within minutes. Furthermore, Spectronaut™ incorporates advanced mProphet algorithms (Nature Methods, 2011) adapted for HRM to enable accurate signal detection and statistically valid peak scoring. The powerful principle behind Spectronaut™ is the integration of the software algorithms with a reagent kit – HRM Calibration Kit – that provides optimal customized parameter calibration for any end-user system independently on the specific chromatography and mass spectrometer set-up. Spectronaut™ is a user-friendly desktop application that can be installed and run on a standard notebook.
Biognosys currently offers its HRM technology as a service for discovery applications and highly multiplexed protein quantification. With the Spectronaut™ release that is free for academic users Biognosys aims to facilitate further development and adoption of HRM technology at the same time supporting open innovation and collaboration between applied and academic research.