Corporate Banner
Satellite Banner
Mass Spectrometry
Scientific Community
Become a Member | Sign in
Home>News>This Article

Thermo Fisher Scientific Launches World’s First “Tribrid” LC-MS System

Published: Tuesday, June 11, 2013
Last Updated: Tuesday, June 11, 2013
Bookmark and Share
Transforming life sciences research with revolutionary depth of analysis and usability.

Thermo Fisher Scientific Inc. has introduced the Thermo Scientific Orbitrap Fusion Tribrid liquid chromatography-mass spectrometry (LC-MS) system, combining its three mass analyzers -quadrupole, Orbitrap and linear ion trap - in a novel “Tribrid” architecture that offers unprecedented depth of analysis of complex biological samples.

The new Orbitrap Fusion LC-MS made its debut at the ASMS Conference on Mass Spectrometry and Allied Topics, being held in Minneapolis from June 9-13 at the Minneapolis Convention Center in booth 154.

“Our mission has been to create the highest-performing commercial mass spectrometer ever built,” said Dr. Ian Jardine, chief technology officer, chromatography and mass spectrometry, Thermo Fisher Scientific.

Dr. Jardine continued, “Additionally, we wanted to make this power widely accessible to the science community and make it incredibly easy to use. In creating the Orbitrap Fusion, we have combined and improved our quadrupole, linear ion trap and Orbitrap technologies in an ultimately flexible research MS system - to arrive at an entirely new class of instrument that will transform our customers’ science.”

“Our largest challenges have been sensitivity and throughput,” explained Dr. Steven Gygi, professor of cell biology at Harvard Medical School. “The Orbitrap Fusion instrument is revolutionary in allowing us to achieve much greater proteome-wide coverage with much greater quantitative accuracy than ever before.”

One way the Orbitrap Fusion system can address throughput challenges is through tandem mass tags (TMT). This technique enables mass spectrometers to determine relative quantification of proteins in multiple samples simultaneously.

The Orbitrap Fusion instrument dramatically increases depth and quality of data compared to previous tools to make TMT results far better.

The new platform takes advantage of MS3 selectivity to improve quantitative accuracy, and can collect twice as many MS3 scans per unit time, at significantly greater sensitivity than was previously possible.

New TMT reagents are also available from Thermo Fisher, allowing comprehensive, simultaneous analysis of up to 10 samples.

At the heart of the Orbitrap Fusion LC-MS is a configuration of three different mass analyzers that work together to raise analytical performance to new levels and enable completely new experimental methods:
• A quadrupole for precursor selection at isolation widths down to 0.4 amu for excellent sensitivity and selectivity;
• An ultra-high-field Orbitrap offering resolution in excess of 450,000 and scan rates up to 15 Hz for unsurpassed selectivity and speed of analysis;
• An ion routing multipole followed by dual-pressure linear ion trap providing MSn HCD, CID and ETD fragmentations and fast, sensitive mass analysis with scan rates of up to 20 Hz. Synchronous precursor selection enhances the instrument’s signal-to-noise ratio.

This Tribrid configuration enables users to positively identify larger numbers of low-abundance proteins faster than previously possible with existing commercial instruments. Its unique architecture enables simultaneous precursor isolation, fragmentation, and data acquisition in both the Orbitrap and linear ion trap mass analyzers. More high-quality data can be collected compared to existing instruments, expanding the range of possible experiments.

The ability to choose between fragmentation modes at any stage of MSn analysis with detection by the Orbitrap or linear ion trap analyzer makes possible a range of novel experiments to achieve a new level of structural information from metabolites, glycans, posttranslational modifications and sequence polymorphisms.

In typical metabolomics experiments, scientists frequently encounter unknowns along with target compounds. To identify the unknowns, the sample must be rerun on an LC-linear ion trap instrument to obtain MSn data.

But it is difficult to match chromatography retention time on the second run, introducing substantial uncertainty. With its unique three mass analyzer configuration, however, the new Orbitrap Fusion Tribrid LC-MS addresses this problem, offering users the ability to transform many small molecule experiments by conclusively identifying unknowns as they are encountered.

Performance with Accessiblity
New intelligent software on the Orbitrap Fusion LC-MS provides Dynamic Scan Management (DSM), a capability that automatically adjusts scan parameters as experiments are running to achieve optimal results.

The Orbitrap Fusion system features a novel, easy-to-use drag and drop method editor that removes most of the guesswork from setting up complex methods. This is part of a comprehensive suite of MS software that supports the platform’s unprecedented usability. It includes:
• Thermo Scientific Freestyle software, a new data visualization tool that also facilitates rapid method development and data quality assessment.
• Thermo Scientific Proteome Discoverer, a comprehensive software application for protein identification.
• Thermo Scientific Compound Discoverer software, used to perform small molecule structural identification in a wide range of applications.
• Thermo Scientific SIEVE software for differential quantitative analysis of proteomic and small molecule samples.
• mzCloud software, a novel mass spectral database that supports identification and structural elucidation of unknowns.

Further Information
Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,800+ scientific posters on ePosters
  • More than 4,000+ scientific videos on LabTube
  • 35 community eNewsletters

Sign In

Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Thermo Fisher Scientific Chosen for Two Awards
Thermo Scientific Vanquish UHPLC System voted Scientists’ Choice Best New Separations Product, Thermo Fisher Scientific voted Reviewers’ Choice Company of the Year.
Thursday, March 12, 2015
Thermo, University of Birmingham Partner to Study Brominated Flame Retardant Pollution
Research aims to increase understanding of the environmental fate and behavior of brominated persistent organic chemicals through deploying recent advances in analytical instrumentation.
Thursday, August 28, 2014
Thermo Fisher Scientific Signs Distribution Agreement with MKI
The agreement sees Thermo become the exclusive worldwide marketer and distributor of MKI’s Lipid Search software for interpretation of lipidomic research data.
Tuesday, July 09, 2013
Thermo Fisher Scientific and Newman-Lakka Institute to Collaborate
The collaboration will research diagnostic approaches for personalized cancer treatment.
Tuesday, June 25, 2013
Thermo Fisher Scientific Reports Second Quarter 2012 Results
Adjusted operating income for the second quarter of 2012 increased 16% compared with the year-ago period.
Thursday, July 26, 2012
Scientific News
High Throughput Mass Spectrometry-Based Screening Assay Trends
Dr John Comley provides an insight into HT MS-based screening with a focus on future user requirements and preferences.
New Method Identifies Up to Twice as Many Proteins and Peptides
An international team of researchers developed a method that identifies up to twice as many proteins and peptides in mass spectrometry data than conventional approaches.
Metabolic Profiles Distinguish Early Stage Ovarian Cancer with Unprecedented Accuracy
Studying blood serum compounds of different molecular weights has led scientists to a set of biomarkers that may enable development of a highly accurate screening test for early-stage ovarian cancer.
Acetaldehyde and Formaldehyde Content in Foods
Korean researchers have determined the content of the toxic and carcinogenic aldehydes, acetaldehyde and formaldehyde, in a variety of food groups.
Combining the Power of Mass Spectrometry & Microscopy
A tool that provides world-class microscopy and spatially resolved chemical analysis shows considerable promise for advancing a number of areas of study, including chemical science, pharmaceutical development and disease progression.
Increasing Vitamin D Supplementation
New study from ETH Zurich finds that elderly women should consume more vitamin D than previously recommended during the winter months.
Advancing Lithium-Ion Battery Research
The increasing number of components in a lithium-ion battery makes it essential to use a variety of analytical methods to attain complete characterization.
Clearest Ever Images of Enzyme that Plays Key Roles in Aging, Cancer
UCLA-led research on telomerase could lead to new strategies for treating disease
Fruit Fly Pheromone Flags Great Real Estate for Starting a Family
Finding could aid efforts to control mosquito-borne diseases like malaria by manipulating odorants
Education and Expense: The Barriers to Mass Spectrometry in Clinical Laboratories?
Here we examine the perceived barriers to mass spec in clinical laboratories and explore the possible drivers behind the recent shift in uptake of the technology in clinical settings.
Scroll Up
Scroll Down
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,800+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,000+ scientific videos