Corporate Banner
Satellite Banner
Mass Spectrometry
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

Thermo Fisher Scientific Launches World’s First “Tribrid” LC-MS System

Published: Tuesday, June 11, 2013
Last Updated: Tuesday, June 11, 2013
Bookmark and Share
Transforming life sciences research with revolutionary depth of analysis and usability.

Thermo Fisher Scientific Inc. has introduced the Thermo Scientific Orbitrap Fusion Tribrid liquid chromatography-mass spectrometry (LC-MS) system, combining its three mass analyzers -quadrupole, Orbitrap and linear ion trap - in a novel “Tribrid” architecture that offers unprecedented depth of analysis of complex biological samples.

The new Orbitrap Fusion LC-MS made its debut at the ASMS Conference on Mass Spectrometry and Allied Topics, being held in Minneapolis from June 9-13 at the Minneapolis Convention Center in booth 154.

“Our mission has been to create the highest-performing commercial mass spectrometer ever built,” said Dr. Ian Jardine, chief technology officer, chromatography and mass spectrometry, Thermo Fisher Scientific.

Dr. Jardine continued, “Additionally, we wanted to make this power widely accessible to the science community and make it incredibly easy to use. In creating the Orbitrap Fusion, we have combined and improved our quadrupole, linear ion trap and Orbitrap technologies in an ultimately flexible research MS system - to arrive at an entirely new class of instrument that will transform our customers’ science.”

“Our largest challenges have been sensitivity and throughput,” explained Dr. Steven Gygi, professor of cell biology at Harvard Medical School. “The Orbitrap Fusion instrument is revolutionary in allowing us to achieve much greater proteome-wide coverage with much greater quantitative accuracy than ever before.”

One way the Orbitrap Fusion system can address throughput challenges is through tandem mass tags (TMT). This technique enables mass spectrometers to determine relative quantification of proteins in multiple samples simultaneously.

The Orbitrap Fusion instrument dramatically increases depth and quality of data compared to previous tools to make TMT results far better.

The new platform takes advantage of MS3 selectivity to improve quantitative accuracy, and can collect twice as many MS3 scans per unit time, at significantly greater sensitivity than was previously possible.

New TMT reagents are also available from Thermo Fisher, allowing comprehensive, simultaneous analysis of up to 10 samples.

At the heart of the Orbitrap Fusion LC-MS is a configuration of three different mass analyzers that work together to raise analytical performance to new levels and enable completely new experimental methods:
• A quadrupole for precursor selection at isolation widths down to 0.4 amu for excellent sensitivity and selectivity;
• An ultra-high-field Orbitrap offering resolution in excess of 450,000 and scan rates up to 15 Hz for unsurpassed selectivity and speed of analysis;
• An ion routing multipole followed by dual-pressure linear ion trap providing MSn HCD, CID and ETD fragmentations and fast, sensitive mass analysis with scan rates of up to 20 Hz. Synchronous precursor selection enhances the instrument’s signal-to-noise ratio.

This Tribrid configuration enables users to positively identify larger numbers of low-abundance proteins faster than previously possible with existing commercial instruments. Its unique architecture enables simultaneous precursor isolation, fragmentation, and data acquisition in both the Orbitrap and linear ion trap mass analyzers. More high-quality data can be collected compared to existing instruments, expanding the range of possible experiments.

The ability to choose between fragmentation modes at any stage of MSn analysis with detection by the Orbitrap or linear ion trap analyzer makes possible a range of novel experiments to achieve a new level of structural information from metabolites, glycans, posttranslational modifications and sequence polymorphisms.

In typical metabolomics experiments, scientists frequently encounter unknowns along with target compounds. To identify the unknowns, the sample must be rerun on an LC-linear ion trap instrument to obtain MSn data.

But it is difficult to match chromatography retention time on the second run, introducing substantial uncertainty. With its unique three mass analyzer configuration, however, the new Orbitrap Fusion Tribrid LC-MS addresses this problem, offering users the ability to transform many small molecule experiments by conclusively identifying unknowns as they are encountered.

Performance with Accessiblity
New intelligent software on the Orbitrap Fusion LC-MS provides Dynamic Scan Management (DSM), a capability that automatically adjusts scan parameters as experiments are running to achieve optimal results.

The Orbitrap Fusion system features a novel, easy-to-use drag and drop method editor that removes most of the guesswork from setting up complex methods. This is part of a comprehensive suite of MS software that supports the platform’s unprecedented usability. It includes:
• Thermo Scientific Freestyle software, a new data visualization tool that also facilitates rapid method development and data quality assessment.
• Thermo Scientific Proteome Discoverer, a comprehensive software application for protein identification.
• Thermo Scientific Compound Discoverer software, used to perform small molecule structural identification in a wide range of applications.
• Thermo Scientific SIEVE software for differential quantitative analysis of proteomic and small molecule samples.
• mzCloud software, a novel mass spectral database that supports identification and structural elucidation of unknowns.


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 3,000+ scientific posters on ePosters
  • More than 4,500+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Thermo Fisher Scientific Chosen for Two Awards
Thermo Scientific Vanquish UHPLC System voted Scientists’ Choice Best New Separations Product, Thermo Fisher Scientific voted Reviewers’ Choice Company of the Year.
Thursday, March 12, 2015
Thermo, University of Birmingham Partner to Study Brominated Flame Retardant Pollution
Research aims to increase understanding of the environmental fate and behavior of brominated persistent organic chemicals through deploying recent advances in analytical instrumentation.
Thursday, August 28, 2014
Thermo Fisher Scientific Signs Distribution Agreement with MKI
The agreement sees Thermo become the exclusive worldwide marketer and distributor of MKI’s Lipid Search software for interpretation of lipidomic research data.
Tuesday, July 09, 2013
Thermo Fisher Scientific and Newman-Lakka Institute to Collaborate
The collaboration will research diagnostic approaches for personalized cancer treatment.
Tuesday, June 25, 2013
Thermo Fisher Scientific Reports Second Quarter 2012 Results
Adjusted operating income for the second quarter of 2012 increased 16% compared with the year-ago period.
Thursday, July 26, 2012
Scientific News
HIV Particles Used to Trap Intact Mammalian Protein Complexes
Belgian scientists from VIB and UGent developed Virotrap, a viral particle sorting approach for purifying protein complexes under native conditions.
Altered Metabolism of Four Compounds Drives Glioblastoma Growth
Findings suggest new ways to treat the malignancy, slow its progression and reveal its extent more precisely.
Coding and Computers Help Spot Methane, Explosives
Coded apertures improve and shrink mass spectrometers for field use.
Why Bearcats Smell Like Buttered Popcorn
Researchers pinpoint chemical compound that gives rare animal its popcorn-like scent.
UCSD Scientists Receive NIH Grant to Combat Antibiotic Resistance
Interdisciplinary program will use systems biology approaches to understand how antibiotics work in concert with patient’s immune system.
Virus Causing Tilapia Die-Offs Identified
Discovery of the virus causing Tilapia die-offs in Israel and Ecuador points the way to protecting a fish that feeds multitudes.
Novel Collagen Fingerprinting Identifies A Neanderthal
Study from the universities of Oxford and Manchester uses ZooMS technique to identify traces of an extinct human.
Hope for Combating Muscular Dystrophy
Decoding a sugar molecule and identifying a mechanism linking it to MS could help in the development of therapy for the disease.
Decoding Sugar Molecules Offers New Key For Combating Muscular Dystrophy
Japanese scientists find a rare sugar unit called ribitol 5-phosphate within the sugar molecules on the surface of muscle cells. Mutations in 3 genes linked to muscular dystrophy affect the creation of this sugar molecule.
A Vision for Precision Medicine
The University of Manchester and the University of Dundee partner to share disease screening data.
Scroll Up
Scroll Down
Skyscraper Banner

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,000+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,500+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!