Corporate Banner
Satellite Banner
Mass Spectrometry
Scientific Community
Become a Member | Sign in
Home>News>This Article

IONICON PTR-MS System Monitors GMP Biotech Processes

Published: Tuesday, January 21, 2014
Last Updated: Tuesday, January 21, 2014
Bookmark and Share
PTR-MS analyze VOCs in real-time and in very low concentrations.

IONICON is a partner in the Austrian Center for Industrial Biotechnology (ACIB) for the development of novel tools for real-time process monitoring of biotechnological fermentations.

After the successful implementation in a laboratory environment, the IONICON PTR-technology could now make another important step and has been utilized for real-time process monitoring in an industrial, GMP (Good Manufacturing Practice) controlled fermentation process.

The ability to monitor volatile microbial metabolites in the off-gas, without an adaptation to the existing process, has enabled the introduction of PTR-MS to this highly regulated area.

Modern biopharmaceutical drugs are produced in biotechnological fermentation processes. Monitoring of these processes is becoming increasingly relevant, especially in the manufacturing sector to promote cost-effective production.

To date, biotechnical processes are generally monitored using off-line analytical procedures. The disadvantages are considerable time delays between sampling and achieving analytical results - often too late for applying corrective actions. Moreover, most off-line procedures are labor- and therefore cost-intensive. Direct real-time analysis overcome these obstacles and lead to more efficient processes as well as enhanced safety of pharmaceutical products.

The Austrian Centre of Industrial Biotechnology (ACIB) has dedicated a work package with a task force of academic partners, partners from the biotech industry and analytical device manufacturers. They have implemented and assessed the suitability of several techniques for real-time fermentation-process monitoring.

IONICON, a partner in ACIB, is the world’s leading producer of real-time gas analyzers based on Proton-Transfer-Reaction Mass-Spectrometry (PTR-MS). A PTR-MS can analyze volatile organic compounds (VOCs) in real-time and in very low concentrations. In a bioreactor, trace amounts of volatile metabolites are emitted as by-products of microbial growth and production.

With PTR-MS real-time monitoring of these VOCs in the bioreactor’s off-gas, it is possible to gauge the activity of these micro-organisms and to gain information for a better control of the fermentation process. Together with the group of Dr. Gerald Striedner at the University of Natural Resources and Life Sciences (BOKU) in Vienna, IONICON has worked on the implementation of PTR-MS for real-time process monitoring, which was reported earlier (see The first step of this implementation has been developed and successfully demonstrated on research-scale bioreactors among several other techniques that have been compared by the researchers.

Industrial fermentation processes are a highly legislated and regulated area following the quality standards laid down in so-called Good Manufacturing Practice (GMP) guidelines. The transfer of a technology that has proven successful in the lab into a GMP process is thus not at all straight forward. Any change to the process, such as the need to install additional probes into the bioreactor, can pose an almost insurmountable obstacle in practice.

“The advantage of the PTR-MS approach is its non-invasive implementation, where highly relevant process information can be gathered from analyzing the fermenter off-gas.”, says Dr. Hubertus Hohenblum from Boehringer Ingelheim, an industrial partner in the ACIB. The implementation is possible without any changes to the actual process. Moreover, the PTR-MS analyzer can be placed outside the GMP area. Among all evaluated real-time technologies, PTR-MS is the first, and so far the only, that has now made an important step to the “real world”.

For the past few weeks an IONICON PTR-MS system had been installed to monitor fermentation processes at the production site of Boehringer Ingelheim RCV GmbH & Co KG in Vienna. The high quality of the gathered data is proven by highly reproducible results. “This is an additional confirmation that demonstrates the suitability of the PTR-MS approach for large-scale, industrial processes”, says biotech researcher Dr. Rene Gutmann from IONICON. Furthermore, the fast analysis of IONICON PTR-MS systems allow for multiplexing to monitoring several fermentations in parallel, which easily justify the initial investment of these high-tech analyzers that are also available on a lease-to-own basis.

Further Information
Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,800+ scientific posters on ePosters
  • More than 4,000+ scientific videos on LabTube
  • 35 community eNewsletters

Sign In

Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

PTR-MS Breath Test Shows Potential for Detecting Liver Disease
Researchers at the University of Birmingham have published results that suggest a non-invasive breath test for liver disease using an IONICON PTR-MS.
Thursday, August 20, 2015
IONICON Selects RJ Lee Group as Exclusive U.S. Distributor
RJ Lee Group has become the exclusive U.S. distribution partner for IONICON’s Trace Gas Analysis products.
Thursday, June 27, 2013
World’s Leading PTR-MS Company IONICON Integrates its Subsidiary
IONICON to celebrate its 15th anniversary in July 2013.
Friday, June 07, 2013
RJ Lee Group's Mobile Lab is Now Deployed for Real-Time VOCs Monitoring
RJ Lee Group equipes mobile lab with IONICON PTR-MS and provides rapid, mobile monitoring of organic contamination and leaks caused by hurricane "Sandy".
Thursday, November 15, 2012
IONICON Announces New Distribution Partner for Australia and New Zealand
IONICON and Ecotech are teaming up.
Wednesday, August 22, 2012
IONICON Announces New Distribution Partner for Singapore, Malaysia and Thailand
IONICON and Omega join efforts in Southeast Asia.
Monday, April 30, 2012
IONICON US Starts its Operations in North America
Effective and fast local PTR-MS sales and support for US customers now directly available from IONICON US, Inc., New Jersey.
Thursday, October 20, 2011
IONICON Announces New Distribution Partner for India
IONICON and MARS Bioanalytical have teamed up to target the Indian market.
Sunday, July 24, 2011
IONICON Appoints New CEO
Mr. Lukas MÄRK stepped in as CEO IONICON Analytik GmbH on June 1, 2011.
Monday, July 11, 2011
Scientific News
High Throughput Mass Spectrometry-Based Screening Assay Trends
Dr John Comley provides an insight into HT MS-based screening with a focus on future user requirements and preferences.
New Method Identifies Up to Twice as Many Proteins and Peptides
An international team of researchers developed a method that identifies up to twice as many proteins and peptides in mass spectrometry data than conventional approaches.
Metabolic Profiles Distinguish Early Stage Ovarian Cancer with Unprecedented Accuracy
Studying blood serum compounds of different molecular weights has led scientists to a set of biomarkers that may enable development of a highly accurate screening test for early-stage ovarian cancer.
Acetaldehyde and Formaldehyde Content in Foods
Korean researchers have determined the content of the toxic and carcinogenic aldehydes, acetaldehyde and formaldehyde, in a variety of food groups.
Combining the Power of Mass Spectrometry & Microscopy
A tool that provides world-class microscopy and spatially resolved chemical analysis shows considerable promise for advancing a number of areas of study, including chemical science, pharmaceutical development and disease progression.
Increasing Vitamin D Supplementation
New study from ETH Zurich finds that elderly women should consume more vitamin D than previously recommended during the winter months.
Advancing Lithium-Ion Battery Research
The increasing number of components in a lithium-ion battery makes it essential to use a variety of analytical methods to attain complete characterization.
Clearest Ever Images of Enzyme that Plays Key Roles in Aging, Cancer
UCLA-led research on telomerase could lead to new strategies for treating disease
Fruit Fly Pheromone Flags Great Real Estate for Starting a Family
Finding could aid efforts to control mosquito-borne diseases like malaria by manipulating odorants
Education and Expense: The Barriers to Mass Spectrometry in Clinical Laboratories?
Here we examine the perceived barriers to mass spec in clinical laboratories and explore the possible drivers behind the recent shift in uptake of the technology in clinical settings.
Scroll Up
Scroll Down
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,800+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,000+ scientific videos