Corporate Banner
Satellite Banner
Mass Spectrometry
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

IONICON PTR-MS System Monitors GMP Biotech Processes

Published: Tuesday, January 21, 2014
Last Updated: Tuesday, January 21, 2014
Bookmark and Share
PTR-MS analyze VOCs in real-time and in very low concentrations.

IONICON is a partner in the Austrian Center for Industrial Biotechnology (ACIB) for the development of novel tools for real-time process monitoring of biotechnological fermentations.

After the successful implementation in a laboratory environment, the IONICON PTR-technology could now make another important step and has been utilized for real-time process monitoring in an industrial, GMP (Good Manufacturing Practice) controlled fermentation process.

The ability to monitor volatile microbial metabolites in the off-gas, without an adaptation to the existing process, has enabled the introduction of PTR-MS to this highly regulated area.

Modern biopharmaceutical drugs are produced in biotechnological fermentation processes. Monitoring of these processes is becoming increasingly relevant, especially in the manufacturing sector to promote cost-effective production.

To date, biotechnical processes are generally monitored using off-line analytical procedures. The disadvantages are considerable time delays between sampling and achieving analytical results - often too late for applying corrective actions. Moreover, most off-line procedures are labor- and therefore cost-intensive. Direct real-time analysis overcome these obstacles and lead to more efficient processes as well as enhanced safety of pharmaceutical products.

The Austrian Centre of Industrial Biotechnology (ACIB) has dedicated a work package with a task force of academic partners, partners from the biotech industry and analytical device manufacturers. They have implemented and assessed the suitability of several techniques for real-time fermentation-process monitoring.

IONICON, a partner in ACIB, is the world’s leading producer of real-time gas analyzers based on Proton-Transfer-Reaction Mass-Spectrometry (PTR-MS). A PTR-MS can analyze volatile organic compounds (VOCs) in real-time and in very low concentrations. In a bioreactor, trace amounts of volatile metabolites are emitted as by-products of microbial growth and production.

With PTR-MS real-time monitoring of these VOCs in the bioreactor’s off-gas, it is possible to gauge the activity of these micro-organisms and to gain information for a better control of the fermentation process. Together with the group of Dr. Gerald Striedner at the University of Natural Resources and Life Sciences (BOKU) in Vienna, IONICON has worked on the implementation of PTR-MS for real-time process monitoring, which was reported earlier (see http://blog.ionicon.com/2012/05/press-conference-successful-implementation-ptrms-bioprocess-monitoring/). The first step of this implementation has been developed and successfully demonstrated on research-scale bioreactors among several other techniques that have been compared by the researchers.

Industrial fermentation processes are a highly legislated and regulated area following the quality standards laid down in so-called Good Manufacturing Practice (GMP) guidelines. The transfer of a technology that has proven successful in the lab into a GMP process is thus not at all straight forward. Any change to the process, such as the need to install additional probes into the bioreactor, can pose an almost insurmountable obstacle in practice.

“The advantage of the PTR-MS approach is its non-invasive implementation, where highly relevant process information can be gathered from analyzing the fermenter off-gas.”, says Dr. Hubertus Hohenblum from Boehringer Ingelheim, an industrial partner in the ACIB. The implementation is possible without any changes to the actual process. Moreover, the PTR-MS analyzer can be placed outside the GMP area. Among all evaluated real-time technologies, PTR-MS is the first, and so far the only, that has now made an important step to the “real world”.

For the past few weeks an IONICON PTR-MS system had been installed to monitor fermentation processes at the production site of Boehringer Ingelheim RCV GmbH & Co KG in Vienna. The high quality of the gathered data is proven by highly reproducible results. “This is an additional confirmation that demonstrates the suitability of the PTR-MS approach for large-scale, industrial processes”, says biotech researcher Dr. Rene Gutmann from IONICON. Furthermore, the fast analysis of IONICON PTR-MS systems allow for multiplexing to monitoring several fermentations in parallel, which easily justify the initial investment of these high-tech analyzers that are also available on a lease-to-own basis.


Further Information
Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,500+ scientific posters on ePosters
  • More than 3,700+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

IONICON Selects RJ Lee Group as Exclusive U.S. Distributor
RJ Lee Group has become the exclusive U.S. distribution partner for IONICON’s Trace Gas Analysis products.
Thursday, June 27, 2013
World’s Leading PTR-MS Company IONICON Integrates its Subsidiary
IONICON to celebrate its 15th anniversary in July 2013.
Friday, June 07, 2013
RJ Lee Group's Mobile Lab is Now Deployed for Real-Time VOCs Monitoring
RJ Lee Group equipes mobile lab with IONICON PTR-MS and provides rapid, mobile monitoring of organic contamination and leaks caused by hurricane "Sandy".
Thursday, November 15, 2012
IONICON Announces New Distribution Partner for Australia and New Zealand
IONICON and Ecotech are teaming up.
Wednesday, August 22, 2012
IONICON Announces New Distribution Partner for Singapore, Malaysia and Thailand
IONICON and Omega join efforts in Southeast Asia.
Monday, April 30, 2012
IONICON US Starts its Operations in North America
Effective and fast local PTR-MS sales and support for US customers now directly available from IONICON US, Inc., New Jersey.
Thursday, October 20, 2011
IONICON Announces New Distribution Partner for India
IONICON and MARS Bioanalytical have teamed up to target the Indian market.
Sunday, July 24, 2011
IONICON Appoints New CEO
Mr. Lukas MÄRK stepped in as CEO IONICON Analytik GmbH on June 1, 2011.
Monday, July 11, 2011
Scientific News
Could a simple saliva test detect Alzheimer's?
Researchers have presented findings suggesting that a simple, non-invasive diagnostic for Alzheimer's could be within reach.
Facebook for the Proteome
Researchers have developed a network for describing protein-protein interactions that can then be used to examine protein interactions that may have biological or clinical significance.
Researchers Reveal Elusive Molecule
A long-standing chemistry puzzle has been solved, with potential implications ranging from industrial processes to atmospheric chemistry.
‘Atomic Detectives’ Scientists that Keep us Safe from Radioactive Criminality
Stephan Richter from the JRC-Institute for Reference Materials and Measurements has examined the merits and limitations of available mass spectrometric instrumentations for nuclear safeguards applications.
MSU Scientists Uncover a ‘Funky Cofactor’
While examining the chemical reactions of bacterial cells in a lab at Michigan State University, researchers found an unusually complex molecule in a protein that completes a simple process.
Aluminum Clusters Shut Down Molecular Fuel Factory
3-D images give clues to extending catalyst life.
Chemists Design a Quantum-Dot Spectrometer
New instrument is small enough to function within a smartphone, enabling portable light analysis.
Creating Bacterial ‘Fight Clubs’ to Discover New Drugs
Creating bacterial “fight clubs” is an effective way to find new drugs from natural sources.
Informatics Tool Helps Scientists Prioritize Protein Modification Research
Researchers have developed a new informatics technology that analyzes existing data repositories of protein modifications and 3D protein structures to help scientists identify and target research on "hotspots" most likely to be important for biological function.
Iron: A Biological Element?
Study shows findings which have meaning for fields as diverse as mining and the search for life in space.
Scroll Up
Scroll Down
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,500+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,700+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FREE!