Corporate Banner
Satellite Banner
Mass Spectrometry
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

IONICON PTR-MS System Monitors GMP Biotech Processes

Published: Tuesday, January 21, 2014
Last Updated: Tuesday, January 21, 2014
Bookmark and Share
PTR-MS analyze VOCs in real-time and in very low concentrations.

IONICON is a partner in the Austrian Center for Industrial Biotechnology (ACIB) for the development of novel tools for real-time process monitoring of biotechnological fermentations.

After the successful implementation in a laboratory environment, the IONICON PTR-technology could now make another important step and has been utilized for real-time process monitoring in an industrial, GMP (Good Manufacturing Practice) controlled fermentation process.

The ability to monitor volatile microbial metabolites in the off-gas, without an adaptation to the existing process, has enabled the introduction of PTR-MS to this highly regulated area.

Modern biopharmaceutical drugs are produced in biotechnological fermentation processes. Monitoring of these processes is becoming increasingly relevant, especially in the manufacturing sector to promote cost-effective production.

To date, biotechnical processes are generally monitored using off-line analytical procedures. The disadvantages are considerable time delays between sampling and achieving analytical results - often too late for applying corrective actions. Moreover, most off-line procedures are labor- and therefore cost-intensive. Direct real-time analysis overcome these obstacles and lead to more efficient processes as well as enhanced safety of pharmaceutical products.

The Austrian Centre of Industrial Biotechnology (ACIB) has dedicated a work package with a task force of academic partners, partners from the biotech industry and analytical device manufacturers. They have implemented and assessed the suitability of several techniques for real-time fermentation-process monitoring.

IONICON, a partner in ACIB, is the world’s leading producer of real-time gas analyzers based on Proton-Transfer-Reaction Mass-Spectrometry (PTR-MS). A PTR-MS can analyze volatile organic compounds (VOCs) in real-time and in very low concentrations. In a bioreactor, trace amounts of volatile metabolites are emitted as by-products of microbial growth and production.

With PTR-MS real-time monitoring of these VOCs in the bioreactor’s off-gas, it is possible to gauge the activity of these micro-organisms and to gain information for a better control of the fermentation process. Together with the group of Dr. Gerald Striedner at the University of Natural Resources and Life Sciences (BOKU) in Vienna, IONICON has worked on the implementation of PTR-MS for real-time process monitoring, which was reported earlier (see http://blog.ionicon.com/2012/05/press-conference-successful-implementation-ptrms-bioprocess-monitoring/). The first step of this implementation has been developed and successfully demonstrated on research-scale bioreactors among several other techniques that have been compared by the researchers.

Industrial fermentation processes are a highly legislated and regulated area following the quality standards laid down in so-called Good Manufacturing Practice (GMP) guidelines. The transfer of a technology that has proven successful in the lab into a GMP process is thus not at all straight forward. Any change to the process, such as the need to install additional probes into the bioreactor, can pose an almost insurmountable obstacle in practice.

“The advantage of the PTR-MS approach is its non-invasive implementation, where highly relevant process information can be gathered from analyzing the fermenter off-gas.”, says Dr. Hubertus Hohenblum from Boehringer Ingelheim, an industrial partner in the ACIB. The implementation is possible without any changes to the actual process. Moreover, the PTR-MS analyzer can be placed outside the GMP area. Among all evaluated real-time technologies, PTR-MS is the first, and so far the only, that has now made an important step to the “real world”.

For the past few weeks an IONICON PTR-MS system had been installed to monitor fermentation processes at the production site of Boehringer Ingelheim RCV GmbH & Co KG in Vienna. The high quality of the gathered data is proven by highly reproducible results. “This is an additional confirmation that demonstrates the suitability of the PTR-MS approach for large-scale, industrial processes”, says biotech researcher Dr. Rene Gutmann from IONICON. Furthermore, the fast analysis of IONICON PTR-MS systems allow for multiplexing to monitoring several fermentations in parallel, which easily justify the initial investment of these high-tech analyzers that are also available on a lease-to-own basis.


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 3,200+ scientific posters on ePosters
  • More than 4,600+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

PTR-MS Breath Test Shows Potential for Detecting Liver Disease
Researchers at the University of Birmingham have published results that suggest a non-invasive breath test for liver disease using an IONICON PTR-MS.
Thursday, August 20, 2015
IONICON Selects RJ Lee Group as Exclusive U.S. Distributor
RJ Lee Group has become the exclusive U.S. distribution partner for IONICON’s Trace Gas Analysis products.
Thursday, June 27, 2013
World’s Leading PTR-MS Company IONICON Integrates its Subsidiary
IONICON to celebrate its 15th anniversary in July 2013.
Friday, June 07, 2013
RJ Lee Group's Mobile Lab is Now Deployed for Real-Time VOCs Monitoring
RJ Lee Group equipes mobile lab with IONICON PTR-MS and provides rapid, mobile monitoring of organic contamination and leaks caused by hurricane "Sandy".
Thursday, November 15, 2012
IONICON Announces New Distribution Partner for Australia and New Zealand
IONICON and Ecotech are teaming up.
Wednesday, August 22, 2012
IONICON Announces New Distribution Partner for Singapore, Malaysia and Thailand
IONICON and Omega join efforts in Southeast Asia.
Monday, April 30, 2012
IONICON US Starts its Operations in North America
Effective and fast local PTR-MS sales and support for US customers now directly available from IONICON US, Inc., New Jersey.
Thursday, October 20, 2011
IONICON Announces New Distribution Partner for India
IONICON and MARS Bioanalytical have teamed up to target the Indian market.
Sunday, July 24, 2011
IONICON Appoints New CEO
Mr. Lukas MÄRK stepped in as CEO IONICON Analytik GmbH on June 1, 2011.
Monday, July 11, 2011
Scientific News
ASMS 2016: Targeting Mass Spectrometry Tools for the Masses
The expanding application range of MS in life sciences, food, energy, and health sciences research was highlighted at this year's ASMS meeting in San Antonio, Texas.
What Makes a Good Scientist?
It’s the journey, not just the destination that counts as a scientist when conducting research.
A New Tool Brings Personalized Medicine Closer
Scientists from EPFL and ETHZ have developed a powerful tool for exploring and determining the inherent biological differences between individuals, which overcomes a major hurdle for personalized medicine.
Rapid Retrieval of Live, Infectious Pathogens from Clinical Samples
An engineered pathogen-binding protein enables rapid isolation of infectious bacteria from joint fluids and accelerates their identification.
Getting a Better Look at How HIV Infects and Takes Over its Host Cells
A new approach, developed by a team of researchers led by The Rockefeller University and The Aaron Diamond AIDS Research Center (ADARC), offers an unprecedented view of how a virus infects and appropriates a host cell, step by step.
Detecting Fake Parmesan Cheeses
Scientists report on a way to catch adulteration of the regional artisanal products.
The Need for Speed
Evaluating MALDI-TOF as a high-throughput screening technology for the pharmaceutical industry.
Process Analysis in Real Time
With a real-time mass spectrometer developed by Fraunhofer researchers, it has become possible for the first time to analyze up to 30 components simultaneously from the gas phase and a liquid, including in-situ analysis.
Triple-Negative Breast Cancer Target Is Found
Researchers at UC Berkeley discover a target that drives cancer metabolism in triple-negative breast cancer.
Plasma Biomarkers for Breast Cancer Diagnosis
Plasma lipidomics profiling identified lipid biomarkers in distinguishing early-stage breast cancer from benign lesions.
Scroll Up
Scroll Down
Skyscraper Banner

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,200+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,600+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!