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Multiplexed Cell- and Bead-based Assays in a No-wash Format for Biologics Screening and Characterization
David Onley, Lucy Chammas, Diana Caracino and Paul G. Wylie

Here we present the results generated from multiplexed no-wash protocols using TTP Labtech’s mirrorball, a laser scanning cytometer.

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Development of a Homogenous RSV Neutralization Assay on the Mirrorball Fluorescence Cytometer: A Simple, Safe and Robust Approach for High-throughput Screening
Anne F. Hammerstein and Lucy Chammas

Here we present the development and implementation of a simple RSV-neutralization assay on TTP Labtech’s mirrorball fluorescence cytometer.

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A Novel Set of Serum-Free, Xeno-Free Differentiation Media for Adipogenesis, Osteogenesis and Chondrogenesis of Human Mesenchymal Stem Cells from Various Tissue Sources
Mira Genser-Nir, Sharon Daniliuc, Marina Tevrovsky, Roni Hazan Brill, Yuliya-Yael Miropolski, David Fiorentini.

An overview of a novel SF, XF differentiation system which enables achieving defined conditions for rapid generation of differentiated hMSCs towards tissue engineering and drug screening applications.

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Platform Agnostic Data Processing Routine for Targeted and Untargeted Metabolite Identification in Drug Discovery
Richard Lee,1 Vitaly Lashin,2 Andrey Paramonov,2 Alexandr Sakharov,2 Alexey Aminov2

A discussion of a new informatics solution that addresses common challenges in metabolite identification and characterization--from LC/MS data processing and metabolites prediction to reporting and databasing of assembled biotransformation knowledge.

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Molecular Abnormalities in Schizophrenia: Neurotransmission, Oxidative Stress, and Apoptosis
Elizabeth J. Want,1 Hendrik Wesseling, 2 Elaine Holmes, 1 and Sabine Bahn 2

A multi-platform approach was employed to analyse distinct brain regions implicated in schizophrenia.

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Accurate Mass Spectral Database: Harnessing the Power of High Performance Mass Spectrometry at Long Last
Lorne Fell, Viatcheslav Artaev, Kevin McNitt, Steve Robles, Albert Lebedev

Standard mixtures comprising of alkanes, PAHs, semivolatiles, and pesticides were analyzed using a high resolution time-of-flight (HRTOF) mass spectrometer—Pegasus GC-HRT (LECO Corporation, Saint Joseph,MI)—at 10 spectra-per-second (m/z40–300) in high resolution mode (25,000 at FWHH). The resulting chromatographic peaks were automatically found, deconvoluted, and curated into an Accurate Mass Library (AML).

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Super-resolution single molecule localization microscopy of the exocytotic machinery underlying insulin secretion.
Deirdre M. Kavanagh, Alison Dun, Rory R. Duncan, and Colin Rickman.

Single molecule imaging of the tSNARE proteins involved in insulin secretion.

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Automated Sample Preparation of Whole Blood for Therapeutic Drug Monitoring and Diagnostics by LC-MS using a Commercial Autosampler
Christian Berchtold1, Irene Wegner1,Timm Hettich1, Reto Bolliger2, Guenter Boehm2, Goetz Schlotterbeck1

In this poster the parameters necessary to automatically prepare whole blood samples for online LC-MS applications in the field of diagnostics and TDM have been investigated. A strategy and the most important parameters are shown for the optimization of a PAL RTC autosampler
for the preparation of whole blood samples.

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Development and Application of Quantitative Immunoassays for Major Milk Allergens Bos d 5 (β-lactoglobulin) and Bos d 11 (β-casein)
1Ross A. R. Yarham, 1Anna Kuklinska-Pijanka MSc, 1David Gillick, 1Elizabeth Young, 2Karine Adel Patient PhD, 2Hervé Bernard PhD, 1Martin D. Chapman PhD, 1James P. Hindley PhD

In this study, we sought to develop accurate, sensitive and reliable assays that would enable quantification of multiple milk allergens.

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Diagnosing Coral disease with Metabolomic Markers
Michael Ochsenkuehn

Application of untargeted metabolomics for the identification of disease markers in corals

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Scientific News
JPK NanoWizard® Applied to a Wide Range of Research
The NanoWizard® AFM from JPK is applied for interdisciplinary research at the University of South Australia for applications including smart wound healing and how plants can protect themselves from toxins.
New Centre Offers Ultra-Speed Protein Analysis
UW-Madison researchers to establish development centre for next-gen protein measurement technologies.
Determination of 13 Organic Toxicants in Human Blood
Researchers have utilised liquid-liquid extraction coupling HPLC-MS/MS to identify and quantify organic toxicants in human blood.
Accessing Metabolic Information with Mass Spec
Scientists at the Helmholtz Zentrum München have developed a new mass spectrometry imaging method which, for the first time, makes it possible to analyze hundreds of metabolites in fixed tissue samples.
Unravelling the Roots of Insect’s Waterproof Coating
Researchers have identified the genes that control cuticular lipid production in Drosophila, by performing an RNAi screen and using Direct Analysis in Real Time and GC-MS.
Massive Helium Discovery a "Game Changer" for Medical Industry
A new development is gas exploration has yielded the discovery of a huge helium gas field, which could help relieve the dwindling supply.
Unidentified Spectra Detector
New algorithm clusters over 250 million spectra for analysis, such that millions of unidentified peptide sequences can be recognised.
Making Metabolite Identification More Efficient
Metasense combines the industry's most-comprehensive metabolic transformation prediction with efficient analysis of LC/MS analytical measurements to identify, visualize, and report chemical biotransformations.
ASMS 2016: Targeting Mass Spectrometry Tools for the Masses
The expanding application range of MS in life sciences, food, energy, and health sciences research was highlighted at this year's ASMS meeting in San Antonio, Texas.
What Makes a Good Scientist?
It’s the journey, not just the destination that counts as a scientist when conducting research.
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