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Wednesday, August 20, 2014
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MS Data Processing With Full Flexibility Of Universal Processing Software
Frans Schoutsen, Barbara van Cann, Vincent Jespers, Michal Godula

Liquid Chromatography (LC) in combination with triple quadrupole Mass Spectrometry (MS) is more and more a common analysis technique in routine laboratories. Especially when using MS/MS selected reaction monitoring (SRM) for a large number of components data handling can be a bottle neck. This poster will show MS data processing of pesticides in water samples using an already established chromatography data system which is now also capable of processing large quantities of MS data.

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Analysis of Dithiocarbamate Pesticides by GC-MS
Soma Dasgupta1,2, Sumaiyya Mujawar1, Kaushik Banerjee1, Hans-Joachim Huebschmann3, Paul Silcock4

Analysis of dithiocarbamate pesticides (DTCs) using an optimized GC-MS method is described. DTCs are not stable and cannot be extracted or analyzed directly. The purpose of this work was develop a reliable method for identification and quantification of residues.

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Analysis of Multiresidue Pesticides Present in Ayurvedic Churna by GC-MS/MS
Manoj Surwade, Sunil T Kumar, Aarti Karkhanis, Manish Kumar, Soma Dasgupta, Hans-Joachim Huebschmann, Paul Silcock, Thermo Fisher Scientific

Analysis of Herbal powders or ‘churnas’ using an optimized GC-MS/MS method is described. As they have high amount of matrix co-extractives, they pose critical analytical problems. The purpose of this work was overcome the effect of the matrix and develop a reliable method.

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Automated Solid-Phase Extraction of Oraganochlorine Pesticides from Drinking Water
Frans Schoustsen and Pranathi R Perati

A new method was developed to automate the capture and elution of Organochlorine pesticides from drinking water. The concentrated analytes were then analyzed by GC with Electron Capture Detection (ECD).

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GC-MS/MS Analysis of Pesticide Residue in Green Tea Extracted by QuEChERS with Acetonitrile as Final Solvent
Cojocariu C., Morgan P., Silcock P., Godula M

QuEChERS involves an initial step when a few grams of the sample are extracted with acetonitrile followed by a clean-up step (with dispersive-SPE) used to remove, to a certain extent, unwanted matrix compound (such as pigments, sugars, organic acids). With QuEChERS, the final extract ends up with the pesticides in acetonitrile, which, being polar solvent, can be
problematic in GC-MS.

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Increasing Extraction Efficiency of Pesticides and Dioxins from Wet Samples Using a Novel Polymer During Accelerated Solvent Extraction
Katerina Bousova, Pranathi Perati, Rahmat Ullah, Kannan Srinivasan

Thermo Scientific™ Dionex™ ASE™ Prep MAP is a proprietary polymer designed to remove moisture and increase extraction efficiencies from wet samples including soils, tissues and food products. This unique formulation allows moisture removal under a variety of ionic strength conditions and accelerated solvent extraction conditions.

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Automatic Characterization of Lipids Using Charge Remote Fragmentation Ions and Peaks Characteristic of Fatty Acid Fragmentation From MALDI MS/MS Data
Ningombam Sanjib Meitei*(1); Arun Apte(2); Dietmar Waidelich(3); Fadi Abdi(4); Matthias Glueckmann(3)

Automated identification of lipids using CRF ions and high energy MS/MS fragment ions and novel software.

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Automatic Characterization of Lipids Using Charge Remote Fragmentation Ions and Peaks Characteristic of Fatty Acid Fragmentation From MALDI MS/MS Data
Ningombam Sanjib Meitei*(1); Arun Apte(2); Dietmar Waidelich(3); Fadi Abdi(4); Matthias Glueckmann(3)

Automated identification of lipids using CRF ions and high energy MS/MS fragment ions and novel software.

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Intact Protein LC-MS: How to Overcome the Challenges?
Robert van Ling, Evert-Jan Sneekes, Sebastiaan Dolman, Laurent Rieux, Mauro DePra, Christian Ravnsborg, Dafydd Milton, Remco Swart

Purpose: Facilitate intact protein LC-MS without compromising performance.
Methods: Construct prototypes of integrated column and sprayer dedicated for intact protein separations and compare performance.
Results: Three prototypes have been directly compared and all showed good protein separations, each with their unique area of strength.

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Instant Connect Gas Sampling Valve Module Introducing a New Flexibility in Gas Sampling for GC and GCMS
Stefano Pelagatti, Paolo Magni, Fausto Pigozzo

Purpose: We present a newly developed gas sampling valve module for the Thermo Scientific™ TRACE™ 1300 Series Gas Chromatograph (GC).

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Scientific News
Embalming Study ‘Rewrites’ Key Chapter in Egyptian History
New evidence to suggest that the origins of mummification started in ancient Egypt 1,500 years earlier than previously thought.
Identification of Psoriatic Arthritis Mediators in Synovial Fluid
This study represents the most comprehensive proteomic analysis of psoriatic arthritis synovial fluid, to date.
Mass Spec-Based Metabolomics Workflows to Evaluate Plant Immunity
This article reviews metabolomics studies used to evaluate plant immunity with an emphasis on mass spectrometry in its many forms.
Using LC-MS to Analyse Sulfonamides in Food Grade Honey
Sulfonamides are one of a number of groups of chemicals whose presence in honey is limited by international regulations.
Reimagining UHPLC
To learn more about the new Vanquish UHPLC system from Thermo Fisher Scientific we spoke to Wulff Niedner, Director of HPLC Product Marketing.
Mass Spec Predicts Improved Response to Anti-HER2 Therapy in Breast Cancer
Immunohistochemistry is the standard technique for detecting HER2 protein expression, it does not provide absolute quantification of this receptor and is prone to false-positives.
Mass Spectrometry Tool Helps Guide Brain Cancer Surgery
A tool to help brain surgeons test and more precisely remove cancerous tissue was successfully used during surgery, according to a Purdue University and Brigham and Women's Hospital study.
Evaluating Acute Hydrogen Sulfide Poisoning in Rats through Serum Metabolomics
Through the use of GC/MS-based metabolomics, researchers have evaluated the effect of acute hydrogen sulfide poisoning on the metabolic profiles of rats.
Simultaneous Determination of Free Urinary Steroids
Researchers have described an LC–MS/MS method that accurate, precise and is in accordance with FDA guidelines.
Human Proteome Project Uncovers 193 Proteins Not Known to Exist
The team of international researchers reports the identification of 193 novel proteins that came from regions of the genome not predicted to code for proteins.
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