Posters

Sequenom EpiTYPER analysis of GNAS methylation in Pseudohypoparathyroidism type Ib patients reveals overall GNAS methylation defects also for the familial cases. Such abnormalities are not detectable via old methodologies such as PCR followed by methylation specific restriction digestion and are here for the first time described.

Nuclear resonance vibrational spectroscopy (NRVS) selectively reveals the complete vibrational density of states (VDOS) of a Mössbauer probe nucleus within a protein. Frequency moments of the VDOS determine effective force constants for 57Fe at the active sites of cytochrome c (cyt c) and deoxymyoglobin (Mb).

To study the outcomes at 6 months after fixed dose 131I therapy for Graves’ disease (GD) with an iodine restricted diet.

Ursolic and betulinic acids acids induce the processes of apoptosis. We tested the risk of osteoporosis induction under influence of triterpenoids, since they can interact with glucocorticoid receptors. In experiments on oviriectomized rats we found that acids do not show any negative influence on percentage of mineral components in the femur and have cholesterol-lowering action under experimental conditions.

a new type of preparative liquid chromatography columns is described with applications of the efficiency

Many problems in analytical spectroscopy require insight from several different techniques to adequately resolve the problem. A common difficulty with using several techniques is often the ability to manage the various data types and making the data available for review, analysis and reporting.

Recognizing differences between related LC/MS data sets is the basic premise for the determination of potential metabolites in drug development. Finding small differences between two or more datasets requires a deep and rigorous analysis of each data set to extract and determine those m/z values that give rise to chromatographic peaks.

Environmentally benign protocols have been developed for the synthesis of various pharmaceutically active heterocycles namely 1,3,4-oxadiazoles, 1,3,4-thiadiazoles, 1,3-dioxanes, pyrazoles, hydrazones and 3,4-dihydropyrimidin-2(1H)-ones, which proceed under the influence of microwave irradiation and using eco-friendly conditions. These greener protocols were catalyzed efficiently by solid supported Nafion®NR50, phosphorus pentasulfide on alumina under solvent-free conditions.

We used Ciphergen technology based on SELDI TOF-MS (Surface Enhanced Laser Desorption/Ionisation Time-of-Flight Mass Spectrometry) for analysis of protein expression profiles of 105 breast cancer tissue samples. The aim of this study was the identification of single proteins or protein patterns specific for different tumour subgroups, which should take advantage of the biomarker as an alternative to commonly used diagnostic and prognostic characteristics.