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Integrated platform including automated bligh and dyer extraction and dual-column
Emmanuel Varesio, Guenter Boehm, Sandra Jahn, Renzo Picenoni, Gérard Hopfgartner

• Automated Bligh and Dyer extraction for metabolomic studies.
• Dual-column UHPLC setup for the analysis of the polar and lipidic fractions.
• Alternating acidic and basic mobile phase for the separation of the polar fraction.
• Identification of unknown compounds by SWATH HR MS2 spectra acquisition.

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Automated protein digestion workflows for MS-based proteomics applications
Gunnar Dittmar1, Oliver Popp1, Guenter Boehm2, Andreas Bruchmann3

The PAL RTC provides an affordable and reliable platform optimised for medium-throughput peptide preparation for shotgun-proteomics based mass spectrometry.
Reduce hands-on time on repetitive work!
Increase consistency!
Increase reproducibility

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Deciphering Regulatory Mechanisms in M. xanthus Using Isotopic Ratio Outlier Analysis (IROA) for Metabolome-wide Quantitation
Daniel Krug 1,2, Carsten Volz 1, Aiko Barsch 3, Chris Beecher 4, Felice de Jong 4, Rolf Müller 1,2

Comprehensive study of regulation in myxobacteria with an untargeted metabolomics setup using Isotopic Ratio Outlier Analysis (IROA) and UHR-Q-TOF

Reliable relative quantitation of known and unknown metabolites from a myxobacterial mutant strain in response to induction of the transcriptional antirepressor taA

Compound identification facilitated by the use of ultra-high resolution MS and the knowledge of the number of carbons in each molecule due to IROA

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Intact Protein LC-MS: How to Overcome the Challenges?
Robert van Ling, Evert-Jan Sneekes, Sebastiaan Dolman, Laurent Rieux, Mauro DePra, Christian Ravnsborg, Dafydd Milton, Remco Swart

Purpose: Facilitate intact protein LC-MS without compromising performance.
Methods: Construct prototypes of integrated column and sprayer dedicated for intact protein separations and compare performance.
Results: Three prototypes have been directly compared and all showed good protein separations, each with their unique area of strength.

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Instant Connect Gas Sampling Valve Module Introducing a New Flexibility in Gas Sampling for GC and GCMS
Stefano Pelagatti, Paolo Magni, Fausto Pigozzo

Purpose: We present a newly developed gas sampling valve module for the Thermo Scientific™ TRACE™ 1300 Series Gas Chromatograph (GC).

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GC-MS/MS Analysis of Pesticide Residues in Green Tea Extracted by QuEChERS with Acetonitrile as Final Solvent
Cojocariu C., Morgan P., Silcock P., Pelagatti S., Magni P. and Pigozzo F.

This poster describes the analysis of several challenging pesticides from green tea samples using GC-MS/MS and acetonitrile as final extraction solvent. The compounds analysed are representatives of various classes of pesticides, such as carboxamids, OC, OP, pyrethriods, aromatic, phenylamides.

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Determination of Veterinary Drug Residues in Milk by GC/MS and GC/ECD
Yu Chongtian, Lv Jianxia

Purpose: Develop a rapid, sensitive method for determining amitraz and its metabolites in milk.
Methods: Samples were hydrolyzed in acidic condition and extracted with hexane. After derivativation, the samples were analyzed by GC-ECD or directly by GC-MS.
Results: The results show that GC-MS determination of amitraz and its metabolites is rapid and sensitive.

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AUTOMATED WORKFLOW FOR THE DETERMINATION OF FATTY ACID METHYL ESTERS (FAME)
Beat Schilling1, Reto Bolliger2, Guenter Boehm2

Transesterification of fatty acid esters with Na-methoxide is a fast, efficient and very robust method for fat analysis in food samples. With the use of three ISs the completeness of the transesterification as well as the extent of undesired saponification can be checked.
The PALworkstation allows to fully automate the FAME preparation, including injection into the GC.

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GC-MS Comparison of Lavindin Grosso Oil Obtained by Steam Distillation and SFE
Claudia Worley, Lisa Zona, Susan Sonchik Marine

Oil was extracted from Lavindin Grosso using steam distillation and using supercritical fluid extraction (SFE). Multiple analyses of the same extract demonstrated repeatability of the analytical method. The variability between batches of distillate bracketed the composition obtained by SFE. The differences among the oils could be attributed more to where the lavender was grown than to the extraction method.

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Showing Results 41 - 50 of 127
Scientific News
Boy or Girl? Lemur Scents Have the Answer
A new study finds that a pregnant lemur’s signature scent depends in part on whether she’s carrying a girl or a boy.
Identification of Fumonisins in Corn
In this study, fumonisin A1, A2, and a3 are identified and quantified by high-resolution liquid chromatography-orbitrap mass spectrometry.
Ground-Breaking Lung Cancer Breath Test In Clinical Trial
University of Leicester and Leicester’s Hospitals to evaluate revolutionary device which detects lung cancer in early stages.
Pittcon Announces Wide Variety of Short Courses
Courses range from beginner and intermediate to advanced levels.
Saliva Test to Diagnose Autism
A spit test may one day be able to diagnose autism according to researchers at Clarkson University and the State University of New York at Plattsburgh.
Improved Accuracy of Pesticide Detection in Food
European Reference Laboratory improves accuracy of pesticide detection in food with AB SCIEX mass spectrometry instrumentation.
A Better Understanding of Earth’s Geological Evolution
New instruments to more precisely measure a uranium element carbon dating ratio of 238U/235U.
Insights Into A Rare Genetic Disease
Study shows mutation in NGLY1 gene is linked to a genetic disorder with severe consequences.
Researchers Study Cultural Heritage Painting Cross-Sections
Study of cultural heritage painting cross-sections using AFM-based nanoscale mass spectrometry technique.
Assessing Tumors During Surgery
A novel biomarker for mutant p53 could help pathologists assessing tumors during surgery.
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