|Intact Protein LC-MS: How to Overcome the Challenges?|
Robert van Ling, Evert-Jan Sneekes, Sebastiaan Dolman, Laurent Rieux, Mauro DePra, Christian Ravnsborg, Dafydd Milton, Remco Swart
Purpose: Facilitate intact protein LC-MS without compromising performance.
Methods: Construct prototypes of integrated column and sprayer dedicated for intact protein separations and compare performance.
Results: Three prototypes have been directly compared and all showed good protein separations, each with their unique area of strength.
|Instant Connect Gas Sampling Valve Module Introducing a New Flexibility in Gas Sampling for GC and GCMS|
Stefano Pelagatti, Paolo Magni, Fausto Pigozzo
Purpose: We present a newly developed gas sampling valve module for the Thermo Scientific™ TRACE™ 1300 Series Gas Chromatograph (GC).
|GC-MS/MS Analysis of Pesticide Residues in Green Tea Extracted by QuEChERS with Acetonitrile as Final Solvent|
Cojocariu C., Morgan P., Silcock P., Pelagatti S., Magni P. and Pigozzo F.
This poster describes the analysis of several challenging pesticides from green tea samples using GC-MS/MS and acetonitrile as final extraction solvent. The compounds analysed are representatives of various classes of pesticides, such as carboxamids, OC, OP, pyrethriods, aromatic, phenylamides.
|Determination of Veterinary Drug Residues in Milk by GC/MS and GC/ECD|
Yu Chongtian, Lv Jianxia
Purpose: Develop a rapid, sensitive method for determining amitraz and its metabolites in milk.
Methods: Samples were hydrolyzed in acidic condition and extracted with hexane. After derivativation, the samples were analyzed by GC-ECD or directly by GC-MS.
Results: The results show that GC-MS determination of amitraz and its metabolites is rapid and sensitive.
|AUTOMATED WORKFLOW FOR THE DETERMINATION OF FATTY ACID METHYL ESTERS (FAME)|
Beat Schilling1, Reto Bolliger2, Guenter Boehm2
Transesterification of fatty acid esters with Na-methoxide is a fast, efficient and very robust method for fat analysis in food samples. With the use of three ISs the completeness of the transesterification as well as the extent of undesired saponification can be checked.
The PALworkstation allows to fully automate the FAME preparation, including injection into the GC.
|GC-MS Comparison of Lavindin Grosso Oil Obtained by Steam Distillation and SFE|
Claudia Worley, Lisa Zona, Susan Sonchik Marine
Oil was extracted from Lavindin Grosso using steam distillation and using supercritical fluid extraction (SFE). Multiple analyses of the same extract demonstrated repeatability of the analytical method. The variability between batches of distillate bracketed the composition obtained by SFE. The differences among the oils could be attributed more to where the lavender was grown than to the extraction method.
|Purification of Synthetic Oligodeoxynucleotides via Catching by Polymerization|
Suntara Fueangfung, Shiyue Fang
This poster describes new simple methods for oligodeoxynucleotide purification without chromotography.
|Chemometrical optimization and fast determination of debittering of table olives by means of capillary electrophoresis|
Albillos SM, Busto MC, Ortega N, Pilar-Izquierdo MC, Ramos-Gómez S, Pérez-Mateos M
Table olives require a thorough process of debittering in order to achieve the organoleptic quality of olives before consumption. Capillary electrophoresis offers several advantages to the determination of the bitter responsible compound oleuropein in table olives, such as speed, reduced amount of solvents and reagents, small amount of sample, high efficiency and lower costs.
|Identification and classification of antifouling compounds secreted by anti MIC microorganisms. A metabolomic analysis.|
Albillos SM; Balaña-Fouce R; Montero O; Barreiro-Mendez C; Blas-Galindo E; Barros-García R; Guedella-Bustamante E; Ullán RV
BIOCORIN project, aims to develop a green alternative to the coatings and solutions used up to date for MIC corrosion control. Some of the results of this project are presented here, with the identification of several relevant antifouling compounds secreted by environmentally isolated anti-MIC strains of microorganisms via a metabolomic approach.