|Microextraction techniques combined with X-ray fluorescence spectrometry for determination of different metal ions in water|
Karina Kocot (a), Beata Zawisza (a), Eva Marguí (b), Ignasi Queralt (c), Rafal Sitko (a)
In present work new preconcentration procedures combining microextraction techniques and X-ray fluorescence spectroscopy were developed. Proposed methodologies are promising tools for multielemental analysis enabling detection of natural pollutants in different water samples.
|Application of Central Composite Design (CCD) for the Optimization of Ultrasound-assisted Extraction (UAE) of Phenolics from Corn (Zea mays)|
Widiastuti Setyaningsih, 1,2 Erwan Duros, 3 Ceferino Carrera, 4 Miguel Palma, 2 Carmelo G. Barroso 2
A new Ultrasound-assisted Extraction (UAE) of phenolic compounds from corn (Zea mays) has been optimized by means of chemometrics tool. The proposed method was validated and successfully applied to the analysis of phenolics content in several corn cultivars.
|Metabolism of Eight Model Pharmaceutical Compounds in Rat and Human HepatoPac Versus Liver Microsomes and Suspension Hepatocyte Platforms|
Julius O. Enoru, William DeMaio, Adiba Watanyar, Kenneth Draper, Amanda Moore & Okey Ukairo
This poster presents our comparative metabolite profiling analysis of eight model pharmaceutical compounds with various biotransformation reactions using a functionally stable model of primary hepatocytes [micropatterned co-cultures (MPCCs)] in parallel with the traditional liver microsomal and suspension hepatocyte systems.
|Quantitative Real-time PCR differentiation between Genetically Modified pollen and Genetically Modified flour in honey|
Eugénia de Andrade1*, Maria Lopes2, Maria Clara Fernandes1, Fátima Quedas2, Isabel Rodrigues1, Amélia Maria Lopes1
We investigated the ability of quantitative real-time PCR, using plasmids calibrants, together with the triploid maize seed endosperm and the haploid pollen, to get good estimates for the copy number of a transgene in relation to the copy number of a species specific gene as a mean to distinguish between pollen and flour from maize.
|Characterization of Proteins and Protein Self Association (Oligomerization) with SEC-MALS|
Michel Terray, Mark Pothecary
In this poster, a series of proteins and their oligomers were characterized using SEC-MALS with UV and RI detection. The monomer molecular weights are measured and compared with those from column calibration. The molecular weights of their oligomers are also compared with column calibration and the differences explored.
|MsCompare: An Untargeted LC & GC/MS Metabolomics Platform for Quality Control, Precise Deconvolution and Data Analysis|
A GC/MS or LC/MS workflow for Metabolomics applications includes a number of distinct steps: experimental design, sampling, sample preparation, data acquisition, data processing, deconvolution, identification and data interpretation.
The MsXelerator software includes Quality Control procedures and Analysis of Variance (ANOVA) to properly control each step in this workflow and to obtain reliable results for both quantitation and identification.
|Searching for Urinary Biomarkers of Exposure to Heterocyclic Amines (HCAs)|
Rosa Busquets, Henrik L. Frandsen, Kerstin Skog
Heterocyclic amines (HCAs) are foodborne carcinogens present in cooked meat and fish, mainly. Metabolites of HCAs have been determined in urine from volunteers following a controlled meal.
|Metabolic Stability Assay Using Human Hepatocyte Co-cultures and Integrated Qualitative/Quantitative High Resolution Mass Spectrometry|
Alex Zang, Ragu Ramanathan, Cornelia Smith, Caroline Lee, Helen Shen, and Zamas Lam
Purpose: To investigate Sequential Window Acquisition of all Theoretical fragment ion spectra (SWATH™) based integrated qualitative and quantitative (qual/quant) assay for simultaneous metabolic stability and metabolite profiling assessments.
|In Vitro Species Comparison Using Long-Term Hepatocyte Co-Cultures Model and Highly Sensitive UHPLC-QTOF-MS with SWATH Analysis|
Jian Yu; Ragu Ramanathan; Cornelia Smith; Caroline Lee; Helen Shen; Zamas Lam
Purpose: To develop a reliable, quicker, and cost-saving in vitro method to accurately predict major human metabolite profile in vivo and to de-risk disproportional or unique human metabolites before a drug candidate nomination