Corporate Banner
Satellite Banner
Technology Networks Header
Sunday, February 01, 2015
Technology Networks
 
Register | Sign in
Home Page>Posters

  Posters

Alteration of urinary metabolic profile in rats treated with methylmercury
Pierre Ayotte, Dinesh Kumar, Pierre-Yves Tremblay, Xiaolei Jin, Sevini Shahbaz, Rekha Mehta, Hing Man Chan, and Oliver Fiehn

A metabolomic approach was used to detect changes in urinary metabolic profile of rats treated orally with methylmercury (MeHg), a ubiquitous fish contaminant with well known neurotoxic properties. An aliquot of urine was collected on day 14, extracted and the extract successively methoxymated and trimethylsilylated prior to analysis by GC/TOF-MS. Results indicate that several metabolic pathways were altered by MeHg treatment, including urea cycle, glutathione metabolism and amino acid degradati

More information
Minor change in the structure major change in the mass spectrum in the example of an N-toluene-pyrrolidine derivative
József Pánczél

MS2 and further MSn spectra of SAR cannot provide sufficient information for metabolite structure elucidation because the masses of fragments in MSn correspond only to separate moieties of SAR. Nevertheless almost the exact structure of a dehydrogenated compound - has a poor MS2 spectrum - could be determined by further fragmentation in an LTQ-Orbitrap instrument and using the MSn mass spectra of an impurity of SAR.

More information
Capture Compounds towards a targeted reduction of proteome complexity
Erik Duelsner, Aysel Alici, Christian Jurinke, Hubert Koester

Capture Compound Mass Spectrometry (CCMS) enables the enrichment of proteins based on their functionality. CCMS is commercialized as kits for research applications and in collaborations with pharmaceutical companies. The focus is on investigating mechanisms of drug action and avoidance of toxic effects of small molecule drugs.

More information
A Novel Approach to Internal Standardization in LC/MS/MS Analysis; Sensitive LC/MS/MS Analysis of Gentamicin
Bruce Babson, Noel Henderson and Nicholas Chestara

A novel approach to internal standardization in LC/MS/MS is demonstrated. Gentamicin, a multi-component aminoglycoside, is derivatized with propyl-d7 chloroformate making the internal standard. Gentamicin components in the samples are derivatized with non-labeled propyl chloroformate making them analogous to the internal standard. Additional benefits of the derivatization are increased hydrophobicity and excellent MS/MS fragmentation.

More information
A novel method for coupling capillary columns
Wil van Egmond, Daniela Peroni,

Using a proprietary, low-melting, deactivated glass we have developed a novel instant Meltfit™ connection. Reliable connectors are made in-situ in less than a minute.

More information
Single stage reversed-phase liquid chromatography-mass spectrometry for the characterization of triglyceride positional isomers.
Haslina Hasan, Brendan J Keely and Julie Wilson

The high level of selectivity afforded by RP-HPLC in combination with CID allowed recognition of eight groups of TAG positional isomers. Development of UPLC approach has reduced the run time by half while maintaining separation. The improved method combined with principal components analysis was used to assess the extent of variation of major TAGs in subcutaneous and intermuscular pork fat from four different locations of adipose tissues.

More information
Five noncovalent peptidic ligands show different affinity rankings in solution and gas phase
Andrey Dyachenko , Michael Goldflam , Marta Vilaseca , Ernest Giralt

Stability of noncovalent complexes of VEGF protein with 5 peptidic ligands is studied. Experiments were conducted in solution (NMR CSP, ITC) and in gas phase (CID TOF MS). Each ligand differs from others in chirality of one amino acid. It was shown, that trend of stability of the studied noncovalent complexes is reversed in the gas phase relatively to the solution. An explanation of this behavior is presented.

More information
Mass-Spectrometric Analysis with Sequenom EpiTYPER of GNAS Methylation in Pseudohypoparathyroydism Type Ib Patients Reveals Overall Methylation Defects also for the Familial Cases
Benedetta Izzi1, Bart Claes2, Diether Lambrechts2, Chris Van Geet1,3, Kathleen Freson1

Sequenom EpiTYPER analysis of GNAS methylation in Pseudohypoparathyroidism type Ib patients reveals overall GNAS methylation defects also for the familial cases. Such abnormalities are not detectable via old methodologies such as PCR followed by methylation specific restriction digestion and are here for the first time described.

More information
Force Spectroscopy of the Iron Atom in Heme Proteins
J. T. Sage, B M. Leu, T. H. Ching, Y. Zhang, J. E. Straub, J. Zhao, W. Sturhahn, E. E. Alp

Nuclear resonance vibrational spectroscopy (NRVS) selectively reveals the complete vibrational density of states (VDOS) of a Mössbauer probe nucleus within a protein. Frequency moments of the VDOS determine effective force constants for 57Fe at the active sites of cytochrome c (cyt c) and deoxymyoglobin (Mb).

More information
<< 6 7 8 9 10 11 12 >>
Showing Results 81 - 90 of 124
Scientific News
A Better Understanding of Earth’s Geological Evolution
New instruments to more precisely measure a uranium element carbon dating ratio of 238U/235U.
Insights Into A Rare Genetic Disease
Study shows mutation in NGLY1 gene is linked to a genetic disorder with severe consequences.
Researchers Study Cultural Heritage Painting Cross-Sections
Study of cultural heritage painting cross-sections using AFM-based nanoscale mass spectrometry technique.
Assessing Tumors During Surgery
A novel biomarker for mutant p53 could help pathologists assessing tumors during surgery.
Pharmaceuticals, Personal Care Products Could Taint Swimming Pools
A new study suggests pharmaceuticals and chemicals from personal care products end up in swimming pools, possibly interacting with chlorine to produce disinfection byproducts with unknown properties and health effects.
More Efficient, Sensitive Estrogen Detection Developed
A new method for detecting trace amounts of estrogen in small samples that holds the potential to improve research into cancer and other diseases has been developed.
Chestnuts Roasting On An Open Fire
ORNL work with scientists funded by The American Chestnut Foundation confirms increased blight-resistance of former forest giant.
Metabolomics Data Normalization with EigenMS
In this study, a singular value decomposition-based method, called EigenMS, for normalization of metabolomics data was tested.
Circadian Variation Of The Human Metabolome
Real-time breath analysis was used to study circadian metabolism.
Cells Build 'Cupboards' To Store Metals
Lawrence Livermore researchers have discovered that cells of the alga Chlamydomonas Reinhardti build a “pantry” to store metal and maintain equilibrium.
Scroll Up
Scroll Down
Skyscraper Banner
SELECTBIO Market Reports
LabTube - Videos for the Scientific Community
eposters - The Online Journal of Scientific Posters
Follow TechNetcom1 on Twitter
Technology Networks Ltd. on LinkedIn