|Five noncovalent peptidic ligands show different affinity rankings in solution and gas phase|
Andrey Dyachenko , Michael Goldflam , Marta Vilaseca , Ernest Giralt
Stability of noncovalent complexes of VEGF protein with 5 peptidic ligands is studied. Experiments were conducted in solution (NMR CSP, ITC) and in gas phase (CID TOF MS). Each ligand differs from others in chirality of one amino acid. It was shown, that trend of stability of the studied noncovalent complexes is reversed in the gas phase relatively to the solution. An explanation of this behavior is presented.
|Mass-Spectrometric Analysis with Sequenom EpiTYPER of GNAS Methylation in Pseudohypoparathyroydism Type Ib Patients Reveals Overall Methylation Defects also for the Familial Cases|
Benedetta Izzi1, Bart Claes2, Diether Lambrechts2, Chris Van Geet1,3, Kathleen Freson1
Sequenom EpiTYPER analysis of GNAS methylation in Pseudohypoparathyroidism type Ib patients reveals overall GNAS methylation defects also for the familial cases. Such abnormalities are not detectable via old methodologies such as PCR followed by methylation specific restriction digestion and are here for the first time described.
|Force Spectroscopy of the Iron Atom in Heme Proteins|
J. T. Sage, B M. Leu, T. H. Ching, Y. Zhang, J. E. Straub, J. Zhao, W. Sturhahn, E. E. Alp
Nuclear resonance vibrational spectroscopy (NRVS) selectively reveals the complete vibrational density of states (VDOS) of a Mössbauer probe nucleus within a protein. Frequency moments of the VDOS determine effective force constants for 57Fe at the active sites of cytochrome c (cyt c) and deoxymyoglobin (Mb).
|No Impact of Dietary Iodine Restriction in Short Term Development of Hypothyroidism Following Fixed Dose Radioactive Iodine Therapy for Graves Disease|
Jubbin J Jacob*, Charles Stephen§, Nihal Thomas*, Regi Oommen¶ and M.S.Seshadri*
To study the outcomes at 6 months after fixed dose 131I therapy for Graves’ disease (GD) with an iodine restricted diet.
|SOME BIOLOGICAL PROPERTIES OF TRITERPENOID ACIDS|
S.N. Morozkina, O.I. Antimonova, E.M. Eremenko*, A.G. Shavva
Ursolic and betulinic acids acids induce the processes of apoptosis. We tested the risk of osteoporosis induction under influence of triterpenoids, since they can interact with glucocorticoid receptors. In experiments on oviriectomized rats we found that acids do not show any negative influence on percentage of mineral components in the femur and have cholesterol-lowering action under experimental conditions.
|the packing of preparative liquid chromatography columns |
a new type of preparative liquid chromatography columns is described with applications of the efficiency
|Achieving Great Science : Bringing Together Physical Measurements and Chemical Analysis|
Michael Boruta, Michel Hachey
Many problems in analytical spectroscopy require insight from several different techniques to adequately resolve the problem. A common difficulty with using several techniques is often the ability to manage the various data types and making the data available for review, analysis and reporting.
|Rapid Metabolite Identification using Advanced Algorithms for Mass Spectral Interpretation|
Mark A. Bayliss, Margaret Antler, Graham McGibbon, Vitaly Lashin
Recognizing differences between related LC/MS data sets is the basic premise for the determination of potential metabolites in drug development. Finding small differences between two or more datasets requires a deep and rigorous analysis of each data set to extract and determine those m/z values that give rise to chromatographic peaks.
|Microwave-Assisted Rapid Access to Bio-active Heterocycles |
Vivek Polshettiwar and Rajender S. Varma
Environmentally benign protocols have been developed for the synthesis of various pharmaceutically active heterocycles namely 1,3,4-oxadiazoles, 1,3,4-thiadiazoles, 1,3-dioxanes, pyrazoles, hydrazones and 3,4-dihydropyrimidin-2(1H)-ones, which proceed under the influence of microwave irradiation and using eco-friendly conditions. These greener protocols were catalyzed efficiently by solid supported Nafion®NR50, phosphorus pentasulfide on alumina under solvent-free conditions.