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Sequence-Specific Ni(II)-Dependent Peptide Bond Hydrolysis for Protein Engineering. Combinatorial Library Determination of Optimal Sequences


The sequence-specific cleavage of the peptide bond is a crucial procedure in protein engineering and purification. The extreme stability of this bond, with half-life for spontaneous hydrolysis estimated as 350-600 years at neutral pH and room temperature, limits the range of appropriate cleavage reagents.

Date Posted: 24/11/2010 00:54:31

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