Sequence-Specific Ni(II)-Dependent Peptide Bond Hydrolysis for Protein Engineering. Combinatorial Library Determination of Optimal Sequences
The sequence-specific cleavage of the peptide bond is a crucial procedure in protein engineering and purification. The extreme stability of this bond, with half-life for spontaneous hydrolysis estimated as 350-600 years at neutral pH and room temperature, limits the range of appropriate cleavage reagents.
