Characterization of Nutrients and Actives in Herbal Supplements and Nutriceuticals Using UHPLC-Multi-Reflecting Time-of-Flight Mass Spectrometry

In this poster, extracts and various supplement dosage forms are introduced via direct infusion or UHPLC separation and analyzed by mass spectrometry (MS). Ultra-high resolution MS was performed using a multi-reflecting time-of-flight MS (MR-TOFMS). The analyses provided chemical fingerprints which are qualitatively and quantitatively distinct. The utility of these resolving powers and acquisition speeds for facile determination of elemental composition and resultant unknown identification is demonstrated. The utility of in-source CID (isCID) to provide supplemental structural information is also presented.