Intact Protein LC-MS: How to Overcome the Challenges?

Mass-spectrometry-based protein characterization finds two major applications in top-down proteomics and biopharma analysis of recombinant proteins, antibodies and other biotherapeutics. However, analysis of high-molecular-weight proteins can be challenging. The methodology chosen should minimize common problems in protein analysis such as degradation, poor recovery, broad elution profiles, poor ionization efficiency, ion sensitivity, and resolution. To address these performance challenges, we compared different prototype column-spray interfaces.