Corporate Banner
Satellite Banner
Mass Spectrometry
Scientific Community
 
Become a Member | Sign in
Home>Resources>Application Notes>This Application Note
  Application Notes
Scientific News
Structural Discoveries Could Aid in Better Drug Design
Scientists have uncovered the structural details of how some proteins interact to turn two different signals into a single integrated output.
New ACE-inhibiting Molecule Found in the Asparagus
Scientists have determined that sulfur-containing compounds in plants can inhibit ACE.
The Linking of Molecules and Microbes
Researchers have succeeded in making antibiotic substances and their bacterial producers simultaneously visible.
PTR-MS Breath Test Shows Potential for Detecting Liver Disease
Researchers at the University of Birmingham have published results that suggest a non-invasive breath test for liver disease using an IONICON PTR-MS.
New Test Could Distinguish Between Bipolar Disorder, Depression
Scientists are developing a test that could tell the difference between bipolar disorder and depression.
Early Detection of Lung Cancer
The University of Manchester has signed a collaboration agreement with Abcodia to perform proteomics studies on a cohort of non-small cell lung cancer cases from the UKCTOCS biobank, with the aim of discovering new blood-based biomarkers for earlier detection of the disease.
Detecting Hidden Ingredients
Researchers from China have used mass spectrometry to reveal the use of undeclared substances in dietary supplements.
How the Lung Repairs its Wounds
Researchers have gained detailed insights into the dynamic remodeling of the tissue during lung repair.
Automation Abound at AACC in Atlanta
Discover the latest breakthroughs, trends and products from the AACC Annual Meeting & Clinical Lab Expo.
Could a simple saliva test detect Alzheimer's?
Researchers have presented findings suggesting that a simple, non-invasive diagnostic for Alzheimer's could be within reach.
Scroll Up
Scroll Down

Automated Sample Preparation of 25-OH-Vitamin D3/D2 from Serum with the AC Extraction Plate and the Tecan Freedom EVO® for the quantification by LC-MS/MS
Bookmark and Share

Tecan Group Ltd.

The current methods for sample preparation include protein precipitation (‘protein crash’), solvent extraction such as liquid-liquid extraction (LLE) and solid-phase extraction (SPE). 

However, all these methods require at least one labor intensive and time-consuming step such as filtration, centrifugation or solvent evaporation. In addition, these steps do not allow straightforward automation of the sample preparation process which is highly desirable in order to cope with the increasing Vitamin D sample load experienced by clinical laboratories. 

The Tecan AC Extraction Plate simplifies the sample preparation step in such a way that no centrifugation, filtration or solvent evaporation steps are needed. In fact the sample preparation procedure is reduced to simple pipetting and shaking. These pipetting and shaking steps were automated on a Tecan Freedom EVO liquid handling system. 

In this application note an approach is described which overcomes the difficulties described above. 


Further Information


Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,500+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,700+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FREE!