Corporate Banner
Satellite Banner
Technology Networks Header
Sunday, September 21, 2014
Technology Networks
 
Register | Sign in
Home Page > Videos > Identification of a Prognostic Protein Profile for Triple Negative Breast Cancer
  Videos

Return

Identification of a Prognostic Protein Profile for Triple Negative Breast Cancer
SelectBio

Arzu Umar, Group Leader, Erasmus Medical Center Rotterdam/Josephine Nefkens Institute

Abstract
Breast cancer is a very heterogeneous disease, consisting of different molecular subtypes. Women with the so-called ‘triple negative’ subtype of breast cancer have poor prognosis and survival compared to other subtypes, due to the aggressive nature of these tumors and current absence of suitable targets for therapy. Evidently, identification of appropriate prognostic protein targets for this group of patients is of vital importance. We have used a comparative tissue proteomics approach for the identification of prognostic protein markers for triple negative breast cancer.For biomarker discovery, we selected 63 fresh frozen primary tumor tissues, of which 25 patients developed local and/or distant metastasis within 60 months (poor prognosis), and 38 remained metastasis free for > 60 months (good prognosis). Tissues were subjected to laser capture microdissection to selectively collect ~4,000 tumor epithelial cells, corresponding to sub-microgram protein, per tissue. Proteins were extracted, trypsin digested and subjected to nanoscale liquid chromatography coupled to high resolution mass spectrometry. Label-free quantitation was performed to retrieve protein abundance data, which served as input for comparative proteome analysis using mixed-effect analysis of variance and leave-one-out cross-validation.Using our tissue proteomics pipeline, we identified a 15-protein predictor that significantly correlates with metastasis free survival (p<0.001)

Request more information
Company product page



For access to this article, enter your email address to instantly recieve a Password Reset link.

Please enter your email address below:

Existing users please Sign In here. Don't have an account? Register Here for free access.

Don't have an account? | Register Here

Scientific News
Mass Spectrometry in Your Hand
Electrospray arrays can dramatically downsize systems and costs for onsite chemical analysis and many other applications.
Study Aims to Develop Breath Test for COPD
A new clinical iniative, lead by NYU Langone Medical Center, aims to determine a breath test’s effectiveness to identify volatile organic compounds (VOCs) in human breath that are biomarkers of chronic obstructive pulmonary disease (COPD).
Comparison of Caffeinated and Decaffeinated Coffee - Implications for Alzheimer's Disease
Researchers from the National University of Singapore successfully demonstrated the viability of GC-TOF-MS as an analytical platform for metabolomics analysis of coffee samples.
Repressing the Repressors May Drive Tissue-Specific Cancers
Stowers scientists establish Drosophila and mammalian models to study mutations found in pediatric brain tumors.
Embalming Study ‘Rewrites’ Key Chapter in Egyptian History
New evidence to suggest that the origins of mummification started in ancient Egypt 1,500 years earlier than previously thought.
Identification of Psoriatic Arthritis Mediators in Synovial Fluid
This study represents the most comprehensive proteomic analysis of psoriatic arthritis synovial fluid, to date.
Mass Spec-Based Metabolomics Workflows to Evaluate Plant Immunity
This article reviews metabolomics studies used to evaluate plant immunity with an emphasis on mass spectrometry in its many forms.
Using LC-MS to Analyse Sulfonamides in Food Grade Honey
Sulfonamides are one of a number of groups of chemicals whose presence in honey is limited by international regulations.
Reimagining UHPLC
To learn more about the new Vanquish UHPLC system from Thermo Fisher Scientific we spoke to Wulff Niedner, Director of HPLC Product Marketing.
Mass Spec Predicts Improved Response to Anti-HER2 Therapy in Breast Cancer
Immunohistochemistry is the standard technique for detecting HER2 protein expression, it does not provide absolute quantification of this receptor and is prone to false-positives.
Scroll Up
Scroll Down
Skyscraper Banner
Skyscraper Banner
Follow TechNetcom1 on Twitter
Technology Networks Ltd. on LinkedIn
Go to LabTube.tv