Corporate Banner
Satellite Banner
Technology Networks Header
Friday, July 25, 2014
Technology Networks
 
Register | Sign in
Home Page > Videos > Identification of a Prognostic Protein Profile for Triple Negative Breast Cancer
  Videos

Return

Identification of a Prognostic Protein Profile for Triple Negative Breast Cancer
SelectBio

Arzu Umar, Group Leader, Erasmus Medical Center Rotterdam/Josephine Nefkens Institute

Abstract
Breast cancer is a very heterogeneous disease, consisting of different molecular subtypes. Women with the so-called ‘triple negative’ subtype of breast cancer have poor prognosis and survival compared to other subtypes, due to the aggressive nature of these tumors and current absence of suitable targets for therapy. Evidently, identification of appropriate prognostic protein targets for this group of patients is of vital importance. We have used a comparative tissue proteomics approach for the identification of prognostic protein markers for triple negative breast cancer.For biomarker discovery, we selected 63 fresh frozen primary tumor tissues, of which 25 patients developed local and/or distant metastasis within 60 months (poor prognosis), and 38 remained metastasis free for > 60 months (good prognosis). Tissues were subjected to laser capture microdissection to selectively collect ~4,000 tumor epithelial cells, corresponding to sub-microgram protein, per tissue. Proteins were extracted, trypsin digested and subjected to nanoscale liquid chromatography coupled to high resolution mass spectrometry. Label-free quantitation was performed to retrieve protein abundance data, which served as input for comparative proteome analysis using mixed-effect analysis of variance and leave-one-out cross-validation.Using our tissue proteomics pipeline, we identified a 15-protein predictor that significantly correlates with metastasis free survival (p<0.001)

Request more information
Company product page



For access to this article, enter your email address to instantly recieve a Password Reset link.

Please enter your email address below:

Existing users please Sign In here. Don't have an account? Register Here for free access.

Don't have an account? | Register Here

Scientific News
Using LC-MS to Analyse Sulfonamides in Food Grade Honey
Sulfonamides are one of a number of groups of chemicals whose presence in honey is limited by international regulations.
Reimagining UHPLC
To learn more about the new Vanquish UHPLC system from Thermo Fisher Scientific we spoke to Wulff Niedner, Director of HPLC Product Marketing.
Mass Spec Predicts Improved Response to Anti-HER2 Therapy in Breast Cancer
Immunohistochemistry is the standard technique for detecting HER2 protein expression, it does not provide absolute quantification of this receptor and is prone to false-positives.
Mass Spectrometry Tool Helps Guide Brain Cancer Surgery
A tool to help brain surgeons test and more precisely remove cancerous tissue was successfully used during surgery, according to a Purdue University and Brigham and Women's Hospital study.
Evaluating Acute Hydrogen Sulfide Poisoning in Rats through Serum Metabolomics
Through the use of GC/MS-based metabolomics, researchers have evaluated the effect of acute hydrogen sulfide poisoning on the metabolic profiles of rats.
Simultaneous Determination of Free Urinary Steroids
Researchers have described an LC–MS/MS method that accurate, precise and is in accordance with FDA guidelines.
Human Proteome Project Uncovers 193 Proteins Not Known to Exist
The team of international researchers reports the identification of 193 novel proteins that came from regions of the genome not predicted to code for proteins.
Comparison of Pesticide Residues in Surface and Ground Water
Measurement of pesticide residues can be used as baseline levels to monitor the future changes and to predict their future impact on the population of the area.
New Innovation in Food Safety Testing
Researchers have used MALDI-TOF MS as a tool to detect Listeria contamination in food.
Whey Beneficially Affects Diabetes and Cardiovascular Disease Risk Factors in Obese Adults
New evidence shores up findings that whey protein could have health benefits for people who are obese and do not yet have diabetes.
Scroll Up
Scroll Down
Skyscraper Banner
Skyscraper Banner
Follow TechNetcom1 on Twitter
Technology Networks Ltd. on LinkedIn
Go to LabTube.tv