Corporate Banner
Satellite Banner
Technology Networks Header
Tuesday, January 27, 2015
Technology Networks
 
Register | Sign in
Home Page > Videos > Mass Spectrometry Imaging of Single Cells in Mouse Models of ALS
  Videos

Return

Mass Spectrometry Imaging of Single Cells in Mouse Models of ALS
Brandeis University

Mass spectrometry (MS) is an indispensable technique in food safety, forensics, pharmaceuticals development, and in the detection of hazardous substances; and its use within the biological sciences is growing exponentially. Whereas traditional MS studies began with sample homogenization, recent technological advances have enabled imaging by mass spectrometry (MSI). Mass spectrometry requires the ionization (charging) of a sample, and this talk presents methods for improving the spatial resolution for ionization that employs lasers and a chemical “matrix” (Matrix-Assisted Laser Desorption Ionization-MALDI). Two novel techniques, both of which increase spatial resolution of MALDI imaging by ~10-fold and enabled the first analysis of single mammalian cells (neurons), will be presented. The first technique involves the simultaneous chemical fixation of tissues and deposition of matrix. The second technique involves the injection of a solution of MALDI matrix only upon cells of interest. Both techniques are compatible with fluorescence microscopy. The application of these techniques to the study of a mouse model of the neurodegenerative disease, Amyotrophic Lateral Sclerosis (ALS) is presented.

Request more information
Company product page



For access to this article, enter your email address to instantly recieve a Password Reset link.

Please enter your email address below:

Existing users please Sign In here. Don't have an account? Register Here for free access.

Don't have an account? | Register Here

Scientific News
A Better Understanding of Earth’s Geological Evolution
New instruments to more precisely measure a uranium element carbon dating ratio of 238U/235U.
Insights Into A Rare Genetic Disease
Study shows mutation in NGLY1 gene is linked to a genetic disorder with severe consequences.
Researchers Study Cultural Heritage Painting Cross-Sections
Study of cultural heritage painting cross-sections using AFM-based nanoscale mass spectrometry technique.
Assessing Tumors During Surgery
A novel biomarker for mutant p53 could help pathologists assessing tumors during surgery.
Pharmaceuticals, Personal Care Products Could Taint Swimming Pools
A new study suggests pharmaceuticals and chemicals from personal care products end up in swimming pools, possibly interacting with chlorine to produce disinfection byproducts with unknown properties and health effects.
More Efficient, Sensitive Estrogen Detection Developed
A new method for detecting trace amounts of estrogen in small samples that holds the potential to improve research into cancer and other diseases has been developed.
Chestnuts Roasting On An Open Fire
ORNL work with scientists funded by The American Chestnut Foundation confirms increased blight-resistance of former forest giant.
Metabolomics Data Normalization with EigenMS
In this study, a singular value decomposition-based method, called EigenMS, for normalization of metabolomics data was tested.
Circadian Variation Of The Human Metabolome
Real-time breath analysis was used to study circadian metabolism.
Cells Build 'Cupboards' To Store Metals
Lawrence Livermore researchers have discovered that cells of the alga Chlamydomonas Reinhardti build a “pantry” to store metal and maintain equilibrium.
Scroll Up
Scroll Down
Skyscraper Banner
SELECTBIO Market Reports
LabTube - Videos for the Scientific Community
eposters - The Online Journal of Scientific Posters
Follow TechNetcom1 on Twitter
Technology Networks Ltd. on LinkedIn