|Validation of a 3-Dimensional Human Liver Microtissue Model for Long-term Hepatotoxicity Studies|
Brad Larson1, Stewart Hunt2, Timothy Moeller3, Diana Long4, and Peter Banks1
Non-steroidal anti-inflammatory drugs (NSAIDs) are a class of drug commonly used as analgesics and antipyretics, as well as for management of rheumatological disorders. They are one of the most highly prescribed drug families around the world, and consequently, along with antimicrobial agents, are the most frequent causes of druginduced liver injury (DILI) (Bjornsson et al., 2010).
|Amino-Coated Metallofullerene Nanoparticles for Glioblastoma Mutiforme Tumor Detection|
Tinghui Li , Susan. Murphy, Kanwarpal Bakshi, Steven LaConte, Zhi Sheng, and Harry Dorn
We report the preparation of a new functionalized trimetallic nitride endohedral metallofullerene, with a cage surface consisting of positively charged amino groups, which is expected to bind more efficiently to negatively charged cell phospholipid bi-layer cellular surfaces and will more readily undergo endocytosis. We now report that this Gd-nanoplatform when subsequently conjugated with an IL-13 peptide, (IL-13-Gd3N@C80(OH)x(NH2)y) exhibits enhanced targeting of U-251 GBM cell lines.
|Performance of a hybrid gamma-optical camera for improved utility in diagnostic imaging|
L.K. Jambi1, J.E. Lees1, S.L. Bugby1, B.S. Bhatia1,2, M.S. Alqahtani1, W.R. McKnight1, A.H. Ng3 and A.C. Perkins3
Performance of a hybrid gamma-optical camera for improved utility in diagnostic imaging
|Side by Side: An Evaluation of 2D vs. 3D Cell Culture for High Throughput Screening in Drug Discovery|
Sophie Quick 1,2, Sinead Knight 1, Jon Winter 3
•3D cell culture has the potential to provide a more physiologically relevant model compared to standard tissue culture plastic.
•From a screening perspective the technology offers the possibility of more predictive drug responses but has an increased cost.
•The question: is it possible and, more importantly, is it worthwhile moving towards screening in High Throughput using a 3D model?
|High Throughput Screening in the European Lead Factory|
S.P. van Helden, W.H. Rutjes, C.A.A. van Boeckel and J.H.M. van den Broek
This paper describes workflows that have been implemented at the screening centre of the European Lead Factory and presents screening statistics on the first 18 months of operation.
|Use of a Microlitre Digital Liquid Handler for Screening Applications|
Joby Jenkins, Gillian Lewis, Wayne Bowen
Digital dispensing offers researchers the most freedom for experimental design and sample placement with each microplate. It makes it relatively simple to plan and execute the most desirable experimental design and not one predicated by manual or automated liquid handling.
|Progressing 3D Spheroid Analysis into a HTS Drug Discovery Method|
Sarah Kessel, Eric Sincoff, Olivier Dery, Lori Fitton
3D Tumorspheroid models for improved predictivity in cancer drug discovery.
|Gut Microbial Metabolites and Hepatic Xenobiotic Metabolism: A High Throughput Screening Approach|
Glynn Martin, James Sidaway, Jonathan Swann
This poster highlights the combination of metabonomics and high throughput screening by the identification of gut microbial metabolites and a screening assay designed to determine their cytotoxicity to liver-like cell cultures.
|Human Cardiomyocytes Derived from Induced Pluripotent Stem Cells: High Throughput and High Content Assessment of Cardiac Toxicity and Drug Efficacy by Monitoring Cytosolic Free Calcium Transients|
1Kettenhofen R, 1Duenbostell A, 2Niedereichholz T, 3D’Angelo JM, 4Horai H, 5Schwengberg S, 1Bohlen H, 6Licher T"
Introduction of selected, pure human cardiomyocytes derived from induced pluripotent stem cell (hiPSCM) into a calcium transient imaging high throughput screening (HTS) assay to assess cardiotoxicity and drug efficacies.