|PRESEPSIN, A SOLUBLE CD14-SUBTYPE, A POSSIBLE NEW BIOMARKER INCREASES IN SEPTIC PATIENTS’ PLASMA FROM PEDIATRIC DEPARTMENT.|
Hayato YAMAGUCHI1), Satoshi KIMURA1), Seiji FUKUOKA1), Emiko NAKAMA1), Hideyasu OTO2), Makoto INOUE2), Takashi SOGA2), Shigetaka KITAZAWA2), Yoh UMEDA2)
Increased plasma concentration of soluble CD14-subtype (presepsin) was observed in pediatric patients with bacteremia. Presepsin could be a possible biomarker of sepsis in pediatric patients, however, their reference interval in children could be lower than that of adults. More studies with larger number of samples are required to confirm the result.
|A Novel Approach Toward Microfluidic Drug Metabolite Synthesis – Electrosynthetic Methodology Simulating Cytochrome (CYP450) Oxidation|
Romain Stalder, Gregory P. Roth and Philip Podmore
A novel microfluidic technology and electrochemical synthesis method is demonstrated for the efficient generation of known drug metabolites. These metabolites are typically generated on first pass hepatic oxidation in vivo. The FLUX Module, a new microfluidic electrochemical cell manufactured by Syrris Ltd., has been employed to generate the metabolites of five commercial drugs: Tolbutamide, Chlorpromazine, Diclofenac, Primidone and Albendazole.
|Exploring the Therapeutic Potential of a Peptide Derived from a Poxviral Immune Evasion Protein|
•9R-VIPER is an effective at TLR4 and TLR2 signal inhibition than VIPER.
•The structure of 9R-VIPER and the mutant peptide L6AE10A were investigated by NMR.
•Important structural information was uncovered and an explanation was found for the loss
of activity when important residues are changed.
|On-chip quantification of miRNA using digital droplet PCR|
Q. Cai1, R.S. Wiederkehr1, B. Jones1, B. Majeed1, T. Stakenborg1, P. Fiorini1, L. Lagae1, M Tsukuda2, T. Matsuno2, I. Yamashita2
miRNAs have a great potential in diagnostics. Hence, automated profiling of miRNAs are of great interest. In-house technology show that it is possible to implement a multiplexing assay for miRNAs on a microfluidic chip using digital droplet PCR.
|High-throughput analysis of protein formulations by DLS|
Sophia Kenrick and Daniel Some
High-throughput analysis of protein formulations by Dynamic Light Scattering (DLS): thermal stability, colloidal stability and a thermal anomaly in the colloidal stability parameter D1
|Digital microphotographic atlas of the adult zebrafish cerebellum|
Kristen Lee, Megan Landy, Ashton Cline, Christopher Corbo, and Zoltan Fulop
Sagittal, coronal and horizontal, one micron thick plastic (semithin) sections of adult zebrafish (Danio rerio) brain were photographed in 40X. The sections were then photomontaged together using in Adobe Photoshop CS5 Extended Version 12.0 to make the photographic atlas.
|Microwave Assisted Green Synthesis of Aspirin From Over The Counter Pain Creams |
Annie E. Dineen, Jodie T. Wasacz
A successful microwave synthesis of methyl salicylate to aspirin using green catalysts from over the counter pain creams. The process was also prepared and tested in an undergraduate organic chemistry lab.
|Analytical Pipetting of Serum|
John Thomas Bradshaw, PhD, Leah Flumerfelt, Richard H. Curtis, PhD, Rachel Parshley
Many types of chemical and biological analyses are based upon analytical techniques involving accurate delivery of liquid components. Pipetting tools have become commonplace especially in many biological and pharmaceutical laboratories. While these tools are familiar to many, their operational differences when pipetting different types of solutions are often over-looked.
|Droplet Microfluidics for 3D Epithelial Cell Culture|
Monika Dolega (Pyzalska), Xavier Gidrol, Nathalie Picollet-D’hahan
The challenge facing 3D cell culture today is to adapt current models to a systems biology approach - in particular, to enable RNA interference-based screens to study the effects of the microenvironment on cellular function.