|Rapid analysis of 3D tumour spheroids in soft agar and on ultra-low attachment plates using a laser scanning imaging system|
Anne F Hammerstein, Diana Caracino, and Paul Wylie
The requirement for better in-vitro models that are compatible with high-throughput screening campaigns has led to the development of 3D cell cultures models, especially muliticellular spheroids, which retain many of the morphological and genetic traits of tumours.
Here we describe the formation of such spheroids by two methods: on ultra-low attachment plates and in semi-solid agarose. Both methods are compatible with 96- and 384-well microplate formats.
|Combining low and high volume liquid handling capabilities for ADME screening|
Joby Jenkins, Kevin Moore, Stephen Fowler, Pascal Schenk
In this study we demonstrate the integration of two liquid handlers to extend the volume dispensing range creating low-volume assay-ready plates with high accuracy and precision. This was then successfully applied to a CYP inhibition assay.
|ADP-Glo™ Kinase Profiling Systems for Targeted and Flexible Kinase Inhibitor Profiling|
Jacquelyn Hennek, Juliano Alves, Said Goueli, and Hicham ZegzoutiPromega Corporation, 2800 Woods Hollow Road, Madison, WI 53711
Drug safety is of paramount importance in the pharmaceutical industry indicating that minimal side effects constitute a major requirement in drug development.
|Recycled Gas Flow Control to Optimize Reaction Conversion and the Accomplishment of Heat Integration in Benzene Production through Hydrodealkylation of Toluene|
Afshin Abrishamkar, Saman Ahmadi Siahpoush, Yury Avramenko, Eiman Rahnema Falavarjani
In this work, a benzene production plant through hydrodealkylation process was investigated through a simulation by Aspen Plus V7.2® and run based on the real data obtained from a benzene production plant operating in the United States.
|Integrating compound storage into automated laboratory workflows |
James Craven, Simon Tullett
This poster discusses the benefits of comPOUND for automated sample storage and delivery. TTP Labtech’s pneumatic transport technology, lab2lab, integrates these stores with other instrumentation into managed, fully automated workflows, allowing the scientist to focus on research and data analysis.
|A mix-and-read cell-based assay for antibody screening against Epithelial Growth Factor Receptor |
Wayne P Bowen, David Onley, Paul Wylie, Diana Caracino and Tristan Cope
Here we present a sensitive robust, mix-and-read method for the screening of antibodies against cell surface proteins. With its simple operation, no-wash format, and high sensitivity, this new method is well-suited for high throughput antibody screening.
|Predictive in silico Screening to Determine Vector-Mediated Transport Properties for the Blood-Brain Barrier Choline Transporter|
Sergey Shityakov and Carola Förster
An attempt to analyze vector-mediated transport properties for the blood-brain barrier choline transporter (BBBCHT) was made to assess and improve drug delivery to the central nervous system.
|Digital Dispensing for Direct Dilution: New Flexibility in Dose-Response Analyses|
Jeff Nielsen, Ken Ward, Christie Dudenhoefer, David Ochs, Joshua Yu, and Kevin
In addition to the economic advantages of improving speed, precision, compound usage and waste, digital dispensing offers scientific advantages. This includes making the execution of complicated experiments easy and fast.
|Identification and Characterization of Strain Specific Drug Target by Subtractive Genome Analysis of Methicillin Resistance Staphylococcus aureus|
Kiran Saeed and Reaz Uddin
This study aimed to identify and characterize strain specific essential protein targets, which will eventually help in the identification of druggable proteins in two strains of MRSA by using different Bioinformatic tools and databases. We adopting a subtractive genome approach within the domain of Computational Biology.