Corporate Banner
Satellite Banner
Metabolomics & Lipidomics
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

Thermo Fisher Scientific and Intrinsic Bioprobes Collaborate to Develop MS Immunoassay Workflow

Published: Friday, July 16, 2010
Last Updated: Friday, July 16, 2010
Bookmark and Share
Turnkey MSIA solution allows researchers to differentiate and quantitate low-abundance intact proteins and their variants.

Thermo Fisher Scientific Inc. and Intrinsic Bioprobes (IBI) Inc., have announced an alliance to co-develop and market a mass spectrometry solution that will enable researchers to perform quantitative, high-throughput, high-resolution protein biomarker assays.

The solution couples the specificity of immunoenrichment with the sensitivity and quantitative capability of mass spectrometry to provide researchers with a complete, higher-resolution view of the proteome. Initially, via an exclusive reseller agreement, the solution will be applied to the detection of human parathyroid hormone (PTH) and its variants, which may be useful as biomarkers for bone diseases and diseases of the endocrine system.

As part of the arrangement, Thermo Fisher Scientific will integrate IBI's proprietary immunoenrichment technology with Thermo Scientific automated sample handling and mass spectrometer systems. Scientists at the Biomarkers Initiatives in Mass Spectrometry (BRIMS) Center, a Thermo Fisher Scientific center of excellence, will collaborate with IBI to develop a mass spectrometric immunoassay (MSIA) workflow for research laboratories. Thermo Fisher Scientific will market, distribute and support the solution and the workflow.

"IBI's approach to biomarker analysis relies on our proprietary MSIA technologies for high-throughput, top-down protein assays," said Dobrin Nedelkov, Ph.D., chief executive officer, Intrinsic Bioprobes. "Our protein assays have been used extensively for biomarker analysis and validation, resulting in the discovery of several novel protein variants with important implications. The combination of our MSIA technology and Thermo Fisher Scientific's robust, automated sample handling, mass spectrometry systems and expertise in clinical research workflows, as well as its global distribution and support, will provide clinical researchers with a turnkey solution."

IBI's proprietary immunoenrichment technology is based on its patented pipette tip that integrates a high-throughput, high-binding-capacity microcolumn activated with antibodies. This technology addresses one of the key challenges of biomarker discovery and validation, the isolation and analysis of very low-abundance proteins such as PTH in complex biological matrices.

A key challenge in proteomics research is the ability to differentiate between intact proteins and their variants.

Traditional enzyme-linked immunosorbent assays (ELISA) are limited by the inability of the antibodies to discriminate between all variants and quantify their abundance. A solution is to use the specificity of mass spectrometry to identify and quantitate microheterogeniety. However, many researchers have been stymied by the lack of an integrated, high-throughput mass spectrometry workflow that is robust and reproducible from laboratory-to-laboratory.

"We are extremely enthusiastic about incorporating IBI's proprietary technology into comprehensive workflows that couple immunoenrichment to the power of high-resolution, quantitative, mass spectrometry," said Mary Lopez, Ph.D., director, Thermo Fisher Scientific's BRIMS Center.

"Over the last year, we have worked closely with IBI to develop a reliable, quantitative assay for parathyroid hormone and its variants. This work, recently published in the Journal of Clinical Chemistry, demonstrates a workflow that enables detection and quantification of very-low-abundance intact PTH, and more importantly, several novel PTH protein variants that cannot be detected using conventional assays."


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 3,100+ scientific posters on ePosters
  • More than 4,500+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Thermo Fisher Q3 Revenues up 31 Percent
Thermo Fisher announced that revenues grew 31% to $4.17 billion for the third quarter ended September 27, 2014.
Thursday, October 23, 2014
Thermo, Nuclea Biotechnologies Collaborate to Develop Diabetes Markers
Companies pooling their expertise to develop novel multiplexed research methods for high-throughput quantification of native insulin and its therapeutic analogs.
Monday, June 02, 2014
Thermo Reports 22% Revenue Rise in Q1 2014
Results include the addition of Life Technologies as of February 4, 2014, and exclude the previously announced related divestitures as of March 22, 2014.
Wednesday, April 23, 2014
Thermo Reports 6% Q4 Revenue Growth
Thermo Fisher Scientific Inc. reported its financial results for the fourth quarter and full year ended December 31, 2013.
Friday, January 31, 2014
Thermo Scientific Forms Technology Alliance Partnership With University of Birmingham
Collaboration aimed at advancing LC-MS techniques in life science research.
Friday, March 29, 2013
Record Revenues for Thermo Fisher Scientific
Revenue for the quarter grew 6% to $3.26 billion in 2012, versus $3.09 billion in 2011.
Thursday, January 31, 2013
Thermo Fisher Scientific and NUS Form Collaboration
Goal to develop mass spectrometry-based techniques to study metabolites.
Monday, January 28, 2013
Thermo Fisher Scientific Launches Maybridge Ro3 Diversity Fragment Library
Diverse set of 1,500 molecules engineered for high-quality fragment screening-based programmes.
Tuesday, July 06, 2010
Thermo Fisher Scientific Collaborates with Leading Proteomics Researcher in Luxembourg
Collaboration aims to develop workflows to overcome current bottlenecks in biomarker discovery and assay development for clinical proteomics research.
Monday, April 26, 2010
Thermo Fisher Scientific, GMU, Johns Hopkins and Toronto’s UHN Extend Collaboration
Accelerating biomarker identification for cancer and other diseases may speed development of diagnostic tests that enable earlier disease detection.
Wednesday, March 31, 2010
Thermo Fisher Scientific Collaborates with Expression Pathology
The collaboration will apply both companies’ expertise for identification of cancer-related proteins.
Friday, December 18, 2009
Thermo Fisher Scientific Names Recipients of RNAi Discovery Grants
More than $400,000 in RNAi screening tools awarded to advance biomedical research and drug discovery.
Friday, November 06, 2009
Thermo Fisher Scientific and Symyx Technologies to Deliver Integrated LIMS and ELN Solutions to Scientists
Thermo Fisher Scientific Inc., today announced the release of a joint product development effort resulting from its partnership with Symyx Technologies.
Tuesday, October 27, 2009
Thermo Fisher Scientific Introduces Linear Ion Trap Trade-Up Program for Single Quadrupole and Ion Trap LC-MS Users
Thermo Fisher Scientific Inc. today announced the introduction of a new program that invites scientists to trade in their single quadrupole or ion trap mass spectrometer - from any vendor - and upgrade to leading Thermo Scientific linear ion trap technology
Wednesday, October 07, 2009
Thermo Fisher Scientific Signs Agreement to Acquire B.R.A.H.M.S. AG
Company acquires provider of specialty in-vitro diagnostic tests for $470 million USD.
Monday, September 07, 2009
Scientific News
New Treatment for Pancreatic Cancer
Researchers at Purdue University have shown how controlling cholesterol metabolism in pancreatic cancer cells reduces metastasis.
Plasma Biomarkers for Breast Cancer Diagnosis
Plasma lipidomics profiling identified lipid biomarkers in distinguishing early-stage breast cancer from benign lesions.
How Different People Respond To Aspirin
Study findings could be used to help identify those who would benefit most from aspirin use.
Altered Metabolism of Four Compounds Drives Glioblastoma Growth
Findings suggest new ways to treat the malignancy, slow its progression and reveal its extent more precisely.
A Metabolic Twist that Drives Cancer Survival
A novel metabolic pathway that helps cancer cells thrive in conditions that are lethal to normal cells has been identified.
Liver-On-Chip Tracks Dynamics of Cellular Function
Hebrew University’s liver-on-chip platform is uniquely able to monitor metabolic changes indicating mitochondrial damage occurring at drug concentrations previously regarded as safe.
Living Off the Fat of the Land
Do cancer cells synthesize the parts for new cells or scavenge them from the environment?
Liver Disease, Obesity Linked
Kanazawa University researchers find similarities in the impeded signalling between central insulin activity and glucose production in the liver for both obese mice and mice that have had the vagus nerve removed.
Decoding Ties Between Vascular Disease, Alzheimer’s
NIH consortium uses big data, team science to uncover complex interplay of factors.
Gene Identified that May Worsen Cancer Outcome
Some patients with breast cancer, lung cancer and leukaemia seem to fare poorly after treatment because of the effects of a particular gene, a new study finds.
SELECTBIO

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,100+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,500+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!