Breakthrough Research Method for the Detection and Absolute Quantification of Metabolites using GC-MS/MS-MRM Technology

Using this advanced triple stage quadrupole GC-MS/MS-MRM technology, scientists are now able to conduct both discovery phase analysis and selective identification and quantification analysis with a single instrument. The high sensitivity and selectivity of the method enables scientists to effectively study plant metabolism and perform phytohormone profiling. The new method is detailed in an application note entitled "Metabolomics strategies using GC-MS/MS technology"

Success in metabolomics, a relatively new area of study that observes the unique chemical fingerprints that cellular processes leave behind, is heavily influenced by the capabilities of analytical instrumentation. This is largely due to the physio-chemical diversity of biological small molecules. Varied and flexible analytical strategies such as GC-MS, LC-MS & MS/MS are therefore essential and often need to be combined. Analysis of low concentration endogenous metabolites in complex biological samples is often challenging due to the chromatographic co-elution of different components.

The use of LC or GC-MS/MS strategies is necessary to improve the selectivity of targeted metabolomics assays.