Corporate Banner
Satellite Banner
Metabolomics & Lipidomics
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

Thermo Fisher Scientific Launches World’s First “Tribrid” LC-MS System

Published: Tuesday, June 11, 2013
Last Updated: Tuesday, June 11, 2013
Bookmark and Share
Transforming life sciences research with revolutionary depth of analysis and usability.

Thermo Fisher Scientific Inc. has introduced the Thermo Scientific Orbitrap Fusion Tribrid liquid chromatography-mass spectrometry (LC-MS) system, combining its three mass analyzers -quadrupole, Orbitrap and linear ion trap - in a novel “Tribrid” architecture that offers unprecedented depth of analysis of complex biological samples.

The new Orbitrap Fusion LC-MS made its debut at the ASMS Conference on Mass Spectrometry and Allied Topics, being held in Minneapolis from June 9-13 at the Minneapolis Convention Center in booth 154.

“Our mission has been to create the highest-performing commercial mass spectrometer ever built,” said Dr. Ian Jardine, chief technology officer, chromatography and mass spectrometry, Thermo Fisher Scientific.

Dr. Jardine continued, “Additionally, we wanted to make this power widely accessible to the science community and make it incredibly easy to use. In creating the Orbitrap Fusion, we have combined and improved our quadrupole, linear ion trap and Orbitrap technologies in an ultimately flexible research MS system - to arrive at an entirely new class of instrument that will transform our customers’ science.”

“Our largest challenges have been sensitivity and throughput,” explained Dr. Steven Gygi, professor of cell biology at Harvard Medical School. “The Orbitrap Fusion instrument is revolutionary in allowing us to achieve much greater proteome-wide coverage with much greater quantitative accuracy than ever before.”

One way the Orbitrap Fusion system can address throughput challenges is through tandem mass tags (TMT). This technique enables mass spectrometers to determine relative quantification of proteins in multiple samples simultaneously.

The Orbitrap Fusion instrument dramatically increases depth and quality of data compared to previous tools to make TMT results far better.

The new platform takes advantage of MS3 selectivity to improve quantitative accuracy, and can collect twice as many MS3 scans per unit time, at significantly greater sensitivity than was previously possible.

New TMT reagents are also available from Thermo Fisher, allowing comprehensive, simultaneous analysis of up to 10 samples.

At the heart of the Orbitrap Fusion LC-MS is a configuration of three different mass analyzers that work together to raise analytical performance to new levels and enable completely new experimental methods:
• A quadrupole for precursor selection at isolation widths down to 0.4 amu for excellent sensitivity and selectivity;
• An ultra-high-field Orbitrap offering resolution in excess of 450,000 and scan rates up to 15 Hz for unsurpassed selectivity and speed of analysis;
• An ion routing multipole followed by dual-pressure linear ion trap providing MSn HCD, CID and ETD fragmentations and fast, sensitive mass analysis with scan rates of up to 20 Hz. Synchronous precursor selection enhances the instrument’s signal-to-noise ratio.

This Tribrid configuration enables users to positively identify larger numbers of low-abundance proteins faster than previously possible with existing commercial instruments. Its unique architecture enables simultaneous precursor isolation, fragmentation, and data acquisition in both the Orbitrap and linear ion trap mass analyzers. More high-quality data can be collected compared to existing instruments, expanding the range of possible experiments.

The ability to choose between fragmentation modes at any stage of MSn analysis with detection by the Orbitrap or linear ion trap analyzer makes possible a range of novel experiments to achieve a new level of structural information from metabolites, glycans, posttranslational modifications and sequence polymorphisms.

In typical metabolomics experiments, scientists frequently encounter unknowns along with target compounds. To identify the unknowns, the sample must be rerun on an LC-linear ion trap instrument to obtain MSn data.

But it is difficult to match chromatography retention time on the second run, introducing substantial uncertainty. With its unique three mass analyzer configuration, however, the new Orbitrap Fusion Tribrid LC-MS addresses this problem, offering users the ability to transform many small molecule experiments by conclusively identifying unknowns as they are encountered.

Performance with Accessiblity
New intelligent software on the Orbitrap Fusion LC-MS provides Dynamic Scan Management (DSM), a capability that automatically adjusts scan parameters as experiments are running to achieve optimal results.

The Orbitrap Fusion system features a novel, easy-to-use drag and drop method editor that removes most of the guesswork from setting up complex methods. This is part of a comprehensive suite of MS software that supports the platform’s unprecedented usability. It includes:
• Thermo Scientific Freestyle software, a new data visualization tool that also facilitates rapid method development and data quality assessment.
• Thermo Scientific Proteome Discoverer, a comprehensive software application for protein identification.
• Thermo Scientific Compound Discoverer software, used to perform small molecule structural identification in a wide range of applications.
• Thermo Scientific SIEVE software for differential quantitative analysis of proteomic and small molecule samples.
• mzCloud software, a novel mass spectral database that supports identification and structural elucidation of unknowns.


Further Information
Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,500+ scientific posters on ePosters
  • More than 3,700+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Thermo Fisher Scientific Signs Distribution Agreement with MKI
The agreement sees Thermo become the exclusive worldwide marketer and distributor of MKI’s Lipid Search software for interpretation of lipidomic research data.
Tuesday, July 09, 2013
Scientific News
New ACE-inhibiting Molecule Found in the Asparagus
Scientists have determined that sulfur-containing compounds in plants can inhibit ACE.
A Metabolic Master Switch Underlying Human Obesity
Researchers find pathway that controls metabolism by prompting fat cells to store or burn fat.
Shedding Light On Century-Old Biochemical Mystery
Yale scientists have used magnetic resonance measurements to show how glucose is metabolized in yeast to answer the puzzle of the “Warburg Effect.”
PTR-MS Breath Test Shows Potential for Detecting Liver Disease
Researchers at the University of Birmingham have published results that suggest a non-invasive breath test for liver disease using an IONICON PTR-MS.
Metabolon and BCM Show Metabolomics May Play Key Role in Precision Medicine
Metabolon’s technology enhances understanding of genetic data and improves health assessment in newly published study.
Newly Discovered Cells Restore Liver Damage in Mice Without Cancer Risk
The liver is unique among organs in its ability to regenerate after being damaged. Exactly how it repairs itself remained a mystery until recently, when researchers supported by the NIH discovered a type of cell in mice essential to the process
Study Finds Cutting Dietary Fat Reduces Body Fat More than Cutting Carbs
In a recent study, restricting dietary fat led to body fat loss at a rate 68 percent higher than cutting the same number of carbohydrate calories when adults with obesity ate strictly controlled diets.
Inappropriate Medical Food Use in Managing Patients with a Type of Metabolic Disorder
Researchers have proposed that there is a need for more rigorous clinical study of dietary management practices for patients with IEMs, including any associated long-term side effects, which may in turn result in the need to reformulate some medical foods.
Medical Researchers a Step Closer to Developing Anti-Obesity Pill
A weight loss pill could soon be possible thanks to the work of Deakin University medical researchers.
Engineered Bacterium Produces Important Industrial Chemical
A Korean research team has reported the production of 1,3-diaminopropane via fermentation of an engineered bacterium.
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,500+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,700+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FREE!