Gut Microbial Metabolites and Hepatic Xenobiotic Metabolism: A High Throughput Screening Approach Glynn Martin, James Sidaway, Jonathan Swann
This poster highlights the combination of metabonomics and high throughput screening by the identification of gut microbial metabolites and a screening assay designed to determine their cytotoxicity to liver-like cell cultures.
Searching for Urinary Biomarkers of Exposure to Heterocyclic Amines (HCAs) Rosa Busquets, Henrik L. Frandsen, Kerstin Skog
Heterocyclic amines (HCAs) are foodborne carcinogens present in cooked meat and fish, mainly. Metabolites of HCAs have been determined in urine from volunteers following a controlled meal.
Metabolic Stability Assay Using Human Hepatocyte Co-cultures and Integrated Qualitative/Quantitative High Resolution Mass Spectrometry Alex Zang, Ragu Ramanathan, Cornelia Smith, Caroline Lee, Helen Shen, and Zamas Lam
Purpose: To investigate Sequential Window Acquisition of all Theoretical fragment ion spectra (SWATH™) based integrated qualitative and quantitative (qual/quant) assay for simultaneous metabolic stability and metabolite profiling assessments.
Mixtures Analysis of Complex Mixtures Michael Bernstein; Carlos Cobas; Santi Domínguez; Manuel Pérez; Agustín Barba
We describe an NMR method to quantify mixture components in wine, edible oils, etc. The method is fully customizable, and amenable to high throughput operation.
A Complete Wine Analysis Using Multiplets Detection Dr Michael Bernstein1; Agustín Barba1; Dr Susanne Klein2; Dr Andrea Dreiseitel2; Daniel Heidger2 and Volker Heidger2
NMR mixtures analysis can be used to determine the concentration of key components in wine. Here we show the analysis using SMA from Mestrelab.
DPPH scavenging activities of selected flavonoids and their mixtures Brachos D.(1)
The aim is to identify the ati-oxidant activity of selected flavonoids (quercetin, luteolin and galangin) and their mixtures using DPPH method including their synergistic or antagonistic interactions.
Correlation between plasma levels of lutein and oxidized low density lipoproteins: a short human intervention study Tiziana Bacchetti, Gianna Ferretti
The increase of plasma carotenoids, in particular lutein, after dietary treatment for two weeks is associated with a significant protective effect against lipid peroxidation of plasma lipoproteins.
Strategic Utilization of Gas Chromatography with Both Nominal and High Resolution Time-of-Flight Mass Spectrometers for Metabolomic Studies David E. Alonso, Jeff Patrick, and Joe Binkley
The Pegasus series of mass spectrometers are indispensable tools for metabolomics research: -Throughput -Discovery -Confirmation GC-HRT analyses resulted in confident metabolite identifications: -Spectral similarity searches -Formulae for fragment, molecular, and adduct ions
Myth Busters: The Truth About Metabolomics and High Resolution Gas Chromatography Time-of-Flight Mass Spectrometry David E. Alonso, Joe Binkley, and Lorne Fell
The data summarized in this presentation dispels myths associated with the utilization of GC-MS for analytes which are critical for metabolomic studies. GC-HRT provides high quality, accurate mass data for: -Spectral similarity searches (NIST, Wiley, etc.) -Formula determination (fragment, molecular & adduct ions) -GC-HRT is an indispensible tool for the analysis of complex biological samples.