Corporate Banner
Satellite Banner
Technology Networks Header
Saturday, August 23, 2014
Technology Networks
 
Register | Sign in
Home Page>Posters

  Posters

Adaptive control of glycerol & methanol feeding in recombinant Pichia pastoris cultures: Impact on antibody titre
Filipe Ataíde, Ana Raquel Ferreira, João J. Clemente, António E. Cunha, Rui Oliveira

Pichia pastoris processes for heterolougous protein expression are typically run in three phases: a batch phase, an exponential feeding fed-batch phase and a long oxygen transfer limitation (OTL) phase. The final protein titre is often limited by the oxygen availability in the OTL phase. Here a direct adaptive controller is designed for the OTL phase. The controller was validated in pilot P. pastoris cultivations expressing a single chain antibody fragment (ScFv). This work shows that the propos

More information
Genetic Algorithms Successfully Select Potential Biomarkers in Mass Spectrometry-Based Metabolomics
Wei Zou, Vladimir Tolstikov

Combination of the comprehensive metabolic profiling utilizing three complementary analytical methods for MS data acquisition and Genetic Algorithms technique for feature selection presenting intriguing avenue for finding and exploration small subsets of strong classifiers.

More information
Bio-analytic silicon chips for the detection of developmentalneurotoxic effects of chemicals and drugs in the context of the European REACH program
S. Buehler , Helene Altrichter , P. J. Koester , C. Tautorat , Ralf Ehret ,Werner Baumann , Jan Gimsa

Bio-analytic silicon chips can be used to investigate stem cell differentiation into neurons and for the in vitro on-line monitoring of cellular reactions under well controlled experimental conditions. Chip systems allow for a parallel, label-free and non-invasive measurement of different parameters by CMOS silicon microsensors by the application of potential neurotoxic and developmental neurotoxic substances.

More information
Bio-analytic silicon chips for the detection of developmentalneurotoxic effects of chemicals and drugs in the context of the European REACH program
S. Buehler , Helene Altrichter , P. J. Koester , C. Tautorat , Ralf Ehret ,Werner Baumann , Jan Gimsa

Bio-analytic silicon chips can be used to investigate stem cell differentiation into neurons and for the in vitro on-line monitoring of cellular reactions under well controlled experimental conditions. Chip systems allow for a parallel, label-free and non-invasive measurement of different parameters by CMOS silicon microsensors by the application of potential neurotoxic and developmental neurotoxic substances.

More information
Biomarkers and Metabolomics: Practical Implication
Vladimir V. Tolstikov

Current presentation is dedicated to case studies and illustrates diversity of the projects. Cases representing different projects combine the same analytical platform we utilize for metabolomics study of blood plasma and urine. While pattern recognition presents certain challenges but rather ones that can be resolved, identification of low abundant unknown biomarkers is critical for the field and offers significant technical challenge in spite of considerable progress in modern analytical techn

More information
Biomarkers and Metabolomics: Practical Implication
Vladimir V. Tolstikov

Current presentation is dedicated to case studies and illustrates diversity of the projects. Cases representing different projects combine the same analytical platform we utilize for metabolomics study of blood plasma and urine. While pattern recognition presents certain challenges but rather ones that can be resolved, identification of low abundant unknown biomarkers is critical for the field and offers significant technical challenge in spite of considerable progress in modern analytical techn

More information
PRIMe: Platform for RIKEN Metabolomics
Atsushi Fukushima, Miyako Kusano, Kenji Akiyama, Takeshi Obayashi, Takayuki Tohge, Masami Yokota Hirai, Shigehiko Kanaya, Masanori Arita, Yoko Shinbo, Kazuo Shinozaki, Tetsuya Sakurai and Kazuki Saito

We have developed a web-based database, "PRIMe (Platform for RIKEN Metabolomics)," which contains powerful tools for researchers to analyze gene co-expression data and mass spectral data. PRIMe has been developed with the main aim of facilitating integrated analysis for transcriptomics and metabolomics.

More information
Revealing Host Metabolome Reprogramming by the Economically Important Fungal Plant Pathogens Botrytis cinerea and Magnaporthe grisea
Aileen R., Smith J., William Allwood, Amanda J. Lloyd, David I. Ellis, Royston Goodacre and Luis A.J. Mur

Metabolomic approaches including Fourier- transform infrared (FT-IR) spectroscopy and Electrospray ionisation mass spectrometry (ESI-MS) were used to elucidate changes during susceptible and resistant interactions of Magnaporthe grisea with B. distachyon. Following data mining, discriminatory analytes (m/z) were shown to be phospholipids (PL) by ESIMS-MS. We are now moving to correlate transcriptional and metabolomic changes, concentrating on PL-based regulation.

More information
Temporal Dynamics of Pathogenesis Related Metabolites and their Metabolic Pathways Following Inoculation of Potato Leaves with Phytophthora infestans
Y. Abu-Nada, A. C. Kushalappa, W. D. Marshall, K. Al-Mughrabi, and A. Murphy

The resistance in potato cultivars against late blight caused by Phytophthora infestans is classified into vertical and horizontal. Several metabolites associated with resistance have been detected: phytoalexins such as rishitin, phytuberin, lubimin, solavetivone etc. Metabolite profiling is a novel technology for the discrimination of quantitative resistance in plants against pathogen stress.

More information
<< 1 2 3 4 5 6 >>
Showing Results 31 - 40 of 55
Scientific News
Single Enzyme is Necessary for Development of Diabetes
12-LO enzyme promotes the obesity-induced oxidative stress in the pancreatic cells.
A New Player in Lipid Metabolism Discovered
Specially engineered mice gained no weight, and normal counterparts became obese on the same high-fat, obesity-inducing Western diet.
UT Southwestern Researchers Uncover New Brain Pathways
New therapies for treating Type 2 diabetes and obesity.
Mass Spec-Based Metabolomics Workflows to Evaluate Plant Immunity
This article reviews metabolomics studies used to evaluate plant immunity with an emphasis on mass spectrometry in its many forms.
Assessing Anticancer Drugs Efficacy by Amino Acid Metabolomics
Researchers aimed to develop a method that determines the therapeutic efficacy of anticancer drugs based on the changes in amino acid metabolism at the cellular level.
Time Of Day Crucial to Accurately Test for Diseases
A new study published in PNAS has found that time of day and sleep deprivation have a significant effect on our metabolism.
When Drugs Do More Harm Than Good for Type 2 Diabetes Patients
Harm to quality of life outweighs benefits of treatment for older patients and those with negative feelings about side effects.
Inflammation in Fat Tissue Helps Prevent Metabolic Disease
The findings were first published online June 12 in Cell Metabolism.
Evaluating Acute Hydrogen Sulfide Poisoning in Rats through Serum Metabolomics
Through the use of GC/MS-based metabolomics, researchers have evaluated the effect of acute hydrogen sulfide poisoning on the metabolic profiles of rats.
Pathway Between Gut and Liver Regulates Bone Mass
Researchers have uncovered a previously unknown biological process involving vitamin B12 and taurine that regulates the production of new bone cells.
Skyscraper Banner
Skyscraper Banner
Follow TechNetcom1 on Twitter
Technology Networks Ltd. on LinkedIn
Go to LabTube.tv