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Predicting hepatotoxicity: Reactive metabolite trapping using glutathione and freshly isolated hepatocytes
Birks, V., Webber, G., Geoffroy, S., Cole, R., and Wood, S.

This poster presents our results to date using clozapine (a compound known to be associated with GSH-adduct formation) as substrate and using stable isotope GSH (GSH13C2,15N) to enhance specificity. In addition, all analyses have been conducted using an Waters Acquity UPLC-MS/MS. Results we have obtained in hepatocytes are compared against findings using human liver microsomes (HLM).

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The Human Serum Metabolome
Nick Psychogios, David Hau, Jun Peng, Igor Sinelnikov, Souhaila Bouatra, Rupasri Mandal, Ram Krishna Murthy, Jianguo Xia, Fiona Bamforth, Janet McManus, Theresa Pedersen, Russ Greiner, Bruce McManus, John Newman and David Wishart

As part of our objective to systematically characterize the human metabolome and advance the fields of quantitative metabolomics, we present a global metabolic profiling of the human serum. Our experimental results indicated that global metabolic profiling methods can routinely detect more than 4200 different compounds in serum.

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Comparison of In Vivo and In Vitro 1-H NMR Spectroscopy in the Rat Brain: Technical Considerations, Effects of Brain Regions and Post Weaning Isolation
Philippine C. Geiszler, A. Napolitano, M.I. Schubert, C.A. Jones, K.C.F. Fone, C.A. Daykin, D.P. Auer

In vivo and in vitro magnetic resonance (MR) spectroscopy are both used to obtain complementary information about the metabolic state of living tissue/tissue extracts, respectively. However, comparisons between in vivo and in vitro measurements are rare. The aim of this study was to compare results from in vivo and in vitro MR spectroscopy to study inter-regional variations and the effects of social isolation on metabolite levels in rat brain.

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Mass-Spectrometric Analysis with Sequenom EpiTYPER of GNAS Methylation in Pseudohypoparathyroydism Type Ib Patients Reveals Overall Methylation Defects also for the Familial Cases
Benedetta Izzi1, Bart Claes2, Diether Lambrechts2, Chris Van Geet1,3, Kathleen Freson1

Sequenom EpiTYPER analysis of GNAS methylation in Pseudohypoparathyroidism type Ib patients reveals overall GNAS methylation defects also for the familial cases. Such abnormalities are not detectable via old methodologies such as PCR followed by methylation specific restriction digestion and are here for the first time described.

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MicroRNA-23b negatively regulates urokinase and c-met and inhibits migration of human hepatocellular carcinoma cells.
Salvi A, Sabelli C, Moncini S, Venturin M, Arici B ,Riva P, Portolani N, Giulini SM, Barlati S and De Petro G.

By bioinformatics we predicted that miR-23b could recognize two sites in the 3’ UTR of uPA (urokinase-type plasminogen activator) and four sites in the 3’ UTR of c-met (hepatocyte growth factor receptor). miR-23b transfections in SKHep1C3 caused uPA and c-met decreased and migration and proliferation inhibition of SKHep1C3; anti-miR-23b transfection in human fibroblasts upregulated uPA and c-met. uPA and c-met shared a common microRNA that negatively regulates their expression.

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Interactive Metabolomics by Diffusion NMR: Improving the Odds of Finding Needles in Haystacks
Jonathan Byrne, Rasmus Bro, Florian Wulfert and Clare A. Daykin

Metabolomics investigates changes in quantities of metabolites. However, blood plasma is a complex, heterogeneous mixture of components which undergo a variety of possible molecular interactions. In particular, many low molecular weight compounds (including drugs) can exist both ‘free’ in solution, bound to proteins or within organised aggregates of macromolecules. To study the effects of e.g. disease on these interactions we have developed a technique termed ‘interactive metabolomics’.

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A compartmented in silico model of Rapeseed central metabolism
Eleftherios Pilalis, Aristotelis Chatziioannou, Fragiskos Kolisis

A large-scale in silico model was constructed for the simulation of the central metabolism of Rapeseed embryos. In order to validate this model, we performed constraint-based Flux Balance Analysis, through application of linear optimization methods. Further exploiting the derived model, an in silico gene deletion analysis and a systemic regulatory analysis by Singular Value Decomposition of the steady-state flux space were performed.

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SYBR® Green I as a biomarker for schizophrenia
Davood Zaeifi, Mehdi Rahmati, Vahab Piranfar

The purpose of this study were to examine if the mRNA of the peripheral dopamine receptor is changed in schizophrenia patients. 50 Naive patients were enrolled. After extracting RNA from white blood cells, cDNA is synthesized. After doing the quantitative Real-time PCR, expression of D3 receptors in healthy persons and patients were compared. Results of this examinations reveals that expression of D3 receptor is increased in compared with controls sample.

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Microfluidic PCR device for diagnostic pathogen detection
Johannes R. Peham, Hannes Steiner, Walter Grienauer, Rudolf Heer, Michael J. Vellekoop, Christa Nöhammer, Herbert Wiesinger

In this work a microfluidic cyclic flow PCR device is presented, which is capable of replicating the bacterial genomic DNA sequence of the 16S ribosomal RNA. The standard laboratory processing time of 3 h could be decreased to 60 min with the microfluidic reactor without loosing PCR efficiency. Integrating an optical fluorescence detector for dsDNA measurement would evolve this device into a micro total analysis system.

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Showing Results 31 - 40 of 67
Scientific News
Newly Identified Biochemical Pathway Could Be Target for Insulin Control
Researchers at Duke Medicine and the University of Alberta are reporting the identification of a new biochemical pathway to control insulin secretion from islet beta cells in the pancreas, establishing a potential target for insulin control.
Dirty,Crusty Meals Fit for (Long-Dormant) Microbes
Researchers apply the latest analytical techniques to further our understanding of desert biocrusts.
CSI -- On The Metabolite's Trail
Bioinformaticians at the University of Jena make the most efficient search engine for molecular structures available online.
Developing a Breathalyzer-Type Low Blood Sugar Warning Device For Diabetes
A multidisciplinary team of researchers at Indiana University-Purdue University Indianapolis has been awarded a $738,000 National Science Foundation grant to develop a breathalyzer-type device to detect the onset of hypoglycemia, or low blood sugar episodes, in people with diabetes.
Identifying The 'Dimmer Switch' Of Diabetes
University of Alberta research gives new insight into what causes Type 2 diabetes.
10 to 1: Bugs Win in NASA study
Bugs are winning out, and that's a good thing according to NASA's Human Research Program.
MYC Oncogene Disrupts Cancers Rhythm
Findings inform time-dependent treatment for reducing side effects and increasing effectiveness of cancer medications.
Keeping Gut Bacteria in Balance Could Help Delay Age-related Diseases
A new study suggests that analyzing intestinal bacteria could be a promising way to predict health outcomes as we age.
Genome Mining Effort Discovers 19 New Natural Products in Four Years
Each of these products is a potential new drug. One of them has already been identified as an antibiotic.
New CRISPR-Cas9 Strategy Edits Genes Two Ways
A team of Harvard and MIT researchers have developed a way to perform genome engineering and gene regulation at the same time.
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