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Temporal Dynamics of Pathogenesis Related Metabolites and their Metabolic Pathways Following Inoculation of Potato Leaves with Phytophthora infestans
Y. Abu-Nada, A. C. Kushalappa, W. D. Marshall, K. Al-Mughrabi, and A. Murphy

The resistance in potato cultivars against late blight caused by Phytophthora infestans is classified into vertical and horizontal. Several metabolites associated with resistance have been detected: phytoalexins such as rishitin, phytuberin, lubimin, solavetivone etc. Metabolite profiling is a novel technology for the discrimination of quantitative resistance in plants against pathogen stress.

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Towards Understanding of Ryegrass-Endophyte Symbiosis through Data Integration of Transcriptome and Metabolome
Mingshu Cao, Linda Johnson, Albert Koulman, Geoff Lane and Susanne Rasmussen

Combined with extensive bioinformatics analyses, our joint analysis of transcriptomics and metabolomics data has generated interesting findings which led to further laboratory verification, such as potential genes associated with alkaloid production.

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Ethnopharmacological Evaluation of Radal (leaves of Lomatia hirsuta) through Metabolite Profiling and Isolation of 2-Methoxyjuglone.
Henrik Toft Simonsena, Louise Berthelsenb, Anne Adsersenb, Søren Brøgger Christensenb, Alfonso Guzmánb and Per Mølgaardb

To evaluate the efficacy of the traditionally used herbal remedy Radal a metabolite profile of the original plant material was established. The EtOAc extract of the leaves and found to be active against the pathogenic fungus Candida albicans (MIC = 8 µg/ml). Cinnamic acid (2) and vanillic acid (5) were identified as the major constituents in the tea by GC-MS along with other phenolic derivatives.

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The Comparison of Lipid Extraction Methods for the Lipidomic Analysis of Human Blood Plasma
Ali Awwad, Catharine Ortori, Dave A. Barrett and Clare A. Daykin

Although metabolomics is and should remain an integrated approach by itself, its complexity requires analogous approaches focused on its components, such as lipidomics. Lipid profiling by NMR without sample extraction, should be seen as only the first stage in lipidomic analysis. Subsequent analysis should involve extraction of the lipids from the biofluid or tissue prior to further profiling using a multi-technique metabolomic approach involving both NMR and MS.

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Profiling of Urinary Metabolites
Vladimir V. Tolstikov, Tobias Kind, Kindra D. Brooks and Robert H. Weiss

Urine samples from healthy individuals and kidney cancer patients were used for metabolic profiling method development and for urine metabolome analysis.

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Biomarker Identification Combining Multivariate Analysis of NMR Spectra with an Innovative Spectral Data Analysis Approach in an Integrated Working Environment
Regis Grenier, Yann Bidault, Chen Peng, Gregory M. Banik, Scott Ramos, Brian Rohrback, Tao Wang and Bin Xia,

A recently developed Metabolomics informatics solution from raw data processing to metabolite database searching, compatible with multiple instruments, has been successfully applied to the analysis of 1H NMR human serum spectra of diabetic and non-diabetic subjects. This study also highlights Overlap Density Heatmap, a novel technology that complements the use of chemometrics in biomarker identification.

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Constructing Directed Metabolic Networks from Microarray Data
J. M. Easton, T. N. Arvanitis, A. Peet and M. Viant

Although it has been several years since metabolic networks became a commonly used analysis technique in bioinformatics, the question of how best to construct them from experimental data is still not satisfactorily resolved. Here we present a method for the construction of directed metabolic networks from microarray datasets using an enhanced version of the KEGG LIGAND database.

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Application of 1H NMR Metabolomics to a Murine Model of Inflammatory Arthritis
Reza Dowlatabadi, B. Joan Miller, Frank R. Jirik, Hans J. Vogel and Aalim M. Weljie

The novel quantitative technique of "Targeted Profiling" of 1H 1D NMR data is applied to a unique model of rheumatoid arthritis. A number of candidate biomarkers not previously identified using NMR techniques are identified related to inflammation and immune response. The pharmaceutical relevance of several of these metabolites is discussed.

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Herbal Metabolic Profiling of Raw and Steamed Panax notoginseng
Eric C.Y. Chan, Swee-Lee Yap, Aik-Jiang Lau, Pay-Chin Leow, Ding-Fung Toh, and Hwee-Ling Koh

At present, metabolite profiling is of growing importance in herbal medicine fields such as breeding, formulation, quality control and clinical trials. Herbal metabolic profiling allows direct detection of down-stream derivatives of metabolites, arising from herbal formulation process, using metabolite profiling ultra performance liquid chromatography time-of-flight mass spectrometry.

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Scientific News
Toxin from Salmonid Fish has Potential to Treat Cancer
Researchers from the University of Freiburg decode molecular mechanism of fish pathogen.
Researchers Find Key Player in Diabetic Kidney Disease Through Power of Metabolomics
Discovery could lead to new and better diagnostic marker for chronic kidney disease.
Can Cell Cycle Protein Prevent or Kill Breast Cancer Tumors?
An MD Anderson study has shown the potential of a simple molecule involved in cancer metabolism as a powerful therapeutic.
Study Reveals Improved Way to Interpret High-Throughput Biological Data
A recent study has revealed a novel workflow, identifying associations between molecules to provide insights into cellular metabolism and gene expression in complex biological systems.
Optical 'Dog's Nose' Developed to Detect Cancer, Other Diseases
Researchers are using optical spectroscopy to develop a quick, non-invasive “breath test” they believe will have the potential to screen for a variety of diseases, including diabetes, infections and cancers.
Researchers Link Liver Disease and Drug Metabolism
Researchers have discovered that nonalcoholic steatohepatitis, an increasingly common but often undiagnosed liver disease, could have significant medical implications for people with type 2 diabetes.
Scientists Create Synthetic Membranes That Grow Like Living Cells
Chemists and biologists at UC San Diego have succeeded in designing and synthesizing an artificial cell membrane capable of sustaining continual growth, just like a living cell.
Potential New Class of Cancer Drugs
Scientists have found a way to stop cancer cell growth by targeting the Warburg Effect, a trait of cancer cell metabolism that scientists have been eager to exploit.
Team Led by TSRI Scientists Shows AIDS Vaccine Candidate Successfully ‘Primes’ Immune System
New research shows that an experimental vaccine candidate can stimulate immune activity necessary to prevent HIV infection.
The Perfect Partnership: Research & Industry; Software & Instrumentation. It really starts to come together at ASMS 2015
Collaboration and knowledge-sharing were evident everywhere: on the bus, in the hallways and in the bars. This article aims to capture this theme and share with you some of the fruits of this coming together of science and industry.
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