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Metabolite profiling of sesquiterpene lactones and phenolics of bioactive extracts from Asteraceae medicinal plants by HPLC-UV-MS
Gobbo-Neto, L.; Silva, V.C.; Passoni, F.D.; Chagas-Paula, D.A.; Oliveira, R.B.; Da Costa, F.B.

Metabolite profiling (HPLC-UV-MS) was performed for extracts from Tithonia diversifolia (Td) and Smallanthus sonchifolius (Ss) leaves [aqueous crude (ACE); leaf rinse (LRE); 70%MeOH rinsed leaves (GFE)]. LREs presented mainly sesquiterpene lactones (SLs) and flavonoids. FGEs and ACEs presented mainly chlorogenic acids. SLs were not detected in GFEs but traces were detected in ACEs. All extracts presented anti-inflammatory potential. LREs were highly toxic while GFEs did not presented significant

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Predicting hepatotoxicity: Reactive metabolite trapping using glutathione and freshly isolated hepatocytes
Birks, V., Webber, G., Geoffroy, S., Cole, R., and Wood, S.

This poster presents our results to date using clozapine (a compound known to be associated with GSH-adduct formation) as substrate and using stable isotope GSH (GSH13C2,15N) to enhance specificity. In addition, all analyses have been conducted using an Waters Acquity UPLC-MS/MS. Results we have obtained in hepatocytes are compared against findings using human liver microsomes (HLM).

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The Human Serum Metabolome
Nick Psychogios, David Hau, Jun Peng, Igor Sinelnikov, Souhaila Bouatra, Rupasri Mandal, Ram Krishna Murthy, Jianguo Xia, Fiona Bamforth, Janet McManus, Theresa Pedersen, Russ Greiner, Bruce McManus, John Newman and David Wishart

As part of our objective to systematically characterize the human metabolome and advance the fields of quantitative metabolomics, we present a global metabolic profiling of the human serum. Our experimental results indicated that global metabolic profiling methods can routinely detect more than 4200 different compounds in serum.

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Comparison of In Vivo and In Vitro 1-H NMR Spectroscopy in the Rat Brain: Technical Considerations, Effects of Brain Regions and Post Weaning Isolation
Philippine C. Geiszler, A. Napolitano, M.I. Schubert, C.A. Jones, K.C.F. Fone, C.A. Daykin, D.P. Auer

In vivo and in vitro magnetic resonance (MR) spectroscopy are both used to obtain complementary information about the metabolic state of living tissue/tissue extracts, respectively. However, comparisons between in vivo and in vitro measurements are rare. The aim of this study was to compare results from in vivo and in vitro MR spectroscopy to study inter-regional variations and the effects of social isolation on metabolite levels in rat brain.

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Mass-Spectrometric Analysis with Sequenom EpiTYPER of GNAS Methylation in Pseudohypoparathyroydism Type Ib Patients Reveals Overall Methylation Defects also for the Familial Cases
Benedetta Izzi1, Bart Claes2, Diether Lambrechts2, Chris Van Geet1,3, Kathleen Freson1

Sequenom EpiTYPER analysis of GNAS methylation in Pseudohypoparathyroidism type Ib patients reveals overall GNAS methylation defects also for the familial cases. Such abnormalities are not detectable via old methodologies such as PCR followed by methylation specific restriction digestion and are here for the first time described.

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MicroRNA-23b negatively regulates urokinase and c-met and inhibits migration of human hepatocellular carcinoma cells.
Salvi A, Sabelli C, Moncini S, Venturin M, Arici B ,Riva P, Portolani N, Giulini SM, Barlati S and De Petro G.

By bioinformatics we predicted that miR-23b could recognize two sites in the 3’ UTR of uPA (urokinase-type plasminogen activator) and four sites in the 3’ UTR of c-met (hepatocyte growth factor receptor). miR-23b transfections in SKHep1C3 caused uPA and c-met decreased and migration and proliferation inhibition of SKHep1C3; anti-miR-23b transfection in human fibroblasts upregulated uPA and c-met. uPA and c-met shared a common microRNA that negatively regulates their expression.

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Interactive Metabolomics by Diffusion NMR: Improving the Odds of Finding Needles in Haystacks
Jonathan Byrne, Rasmus Bro, Florian Wulfert and Clare A. Daykin

Metabolomics investigates changes in quantities of metabolites. However, blood plasma is a complex, heterogeneous mixture of components which undergo a variety of possible molecular interactions. In particular, many low molecular weight compounds (including drugs) can exist both ‘free’ in solution, bound to proteins or within organised aggregates of macromolecules. To study the effects of e.g. disease on these interactions we have developed a technique termed ‘interactive metabolomics’.

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A compartmented in silico model of Rapeseed central metabolism
Eleftherios Pilalis, Aristotelis Chatziioannou, Fragiskos Kolisis

A large-scale in silico model was constructed for the simulation of the central metabolism of Rapeseed embryos. In order to validate this model, we performed constraint-based Flux Balance Analysis, through application of linear optimization methods. Further exploiting the derived model, an in silico gene deletion analysis and a systemic regulatory analysis by Singular Value Decomposition of the steady-state flux space were performed.

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SYBR® Green I as a biomarker for schizophrenia
Davood Zaeifi, Mehdi Rahmati, Vahab Piranfar

The purpose of this study were to examine if the mRNA of the peripheral dopamine receptor is changed in schizophrenia patients. 50 Naive patients were enrolled. After extracting RNA from white blood cells, cDNA is synthesized. After doing the quantitative Real-time PCR, expression of D3 receptors in healthy persons and patients were compared. Results of this examinations reveals that expression of D3 receptor is increased in compared with controls sample.

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Scientific News
How it Works: Advanced Data Analysis Using Visualization
Visualisation of data can be used to help molecular biologists tackle the vast datasets their experiments create.
Cell Metabolism Linked to Spread of Cancer
Scientists discover macrophage metabolism can be attuned to prevent the spread of cancer.
Integrated Omics Analysis
Studying multi-omics promises to give a more holistic picture of the organism and its place in its ecosystem, however despite the complexities involved those within the field are optimistic.
Fatty Liver Disease Linked to Type 2 Diabetes
Recent study identifies factors causing insulin to misbehave in non-alcoholic fatty liver disease.
New Pathway for COPD Biomarker Development
A study from Philip Morris International has highlighted multi-lipid profiling as a potential new pathway for COPD biomarker development.
Lab-on-a-Chip Detects Effects of Poison
A fast and efficient mixer has been developed for testing the effect of toxic substances faster by using a new lab-on-a-chip.
Clinical Trial Finds Medicine Program Alters Blood Serum
Meditation, yoga and vegetarian diet linked to decline in plasma metabolites associated with inflammation and cardiovascular disease risk.
3D-Printing in Science: Conference Co-Staged with LABVOLUTION
LABVOLUTION 2017 will have an added highlight of a simultaneous conference, "3D-Printing in Science".
Targeting Cardiovascular Disease Risk Factors May be Important Across a Lifetime
The study suggests efforts to prevent risk factors should extend to those older than 65.
Beige Fat Formation Linked to Anti-diabetic Effect
Researchers at UTSW have found that the protein connexin 43 forms cell-to-cell communication channels on the surface of emerging beige fat cells that amplify the signals from those few nerve fibers.
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