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Friday, December 19, 2014
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Webcasts
Characterizing the Early Drug Development Outsourcing Landscape Characterizing the Early Drug Development Outsourcing Landscape
Stella Stergiopoulos, Tufts Center for the Study of Drug Design, speaking at Drug Discovery and Development 2014
Recent Advancement in Mendelian Genomics and Data Management/Analysis Challenges at the Yale Center for Genome Analysis.Recent Advancement in Mendelian Genomics and Data Management/Analysis Challenges at the Yale Center for Genome Analysis.
Shrikant Mane, Yale Center for Genome Analysis, speaking at Advances in NGS & Big Data 2014

Market Reports
Gene Expression Analysis Market by Technology, Consumables, Services & Applications - Global Forecast to 2018
MarketsandMarkets

Over the years, the gene expression analysis market, comprising of instruments, consumables, and services, has witnessed various technological advancements. These advancements have led to a growth in the number of gene expression applications.
DNA Sequencing
RI Technologies

This report presents an in depth analysis of DNA sequencing market. The study emphasizes on both, the methods and instruments/software of DNA sequencing in medical research, agriculture and animal husbandry and DNA testing and forensics application areas.
Big Data Market in India 2013
Netscribes

The report highlights the current as well as the future big data market scenario in India. The report furnishes explicit details on the crucial aspects of the overall market to provide a holistic view, with the aide of crisp and insightful representation of market data.
Life Science Software 2013: Markets and Opportunities
Biopharm Reports

This reports into in the life science field examines the use of specialist software and databases relating to the conduct of PCR, DNA sequencing, DNA microarray, flow cytometry, mass spectrometry and disease biomarker studies. The studies were carried out to assist software developers and vendors to identify market opportunities in these fields.
DNA Sequencing 2013: Market Developments, Growth Areas and Opportunities
Biopharm Reports

DNA sequencing is important in all areas of biological research and increasing demand for this technique is driving advances in this field, in the form of next-generation techniques. Today, laboratories use a number of different sequencing methods, however common goals include increasing throughputs and speeds, while reducing costs.
Life Science Consumables 2013: Markets and Opportunities
Biopharm Reports

Techniques such as PCR, DNA microarray, DNA sequencing, mass spectrometry and flow cytometry have grown significantly in the last decade and by 2015 markets for these five areas are expected to exceed $50 billion, globally. In these and related fields, consumables are generally the second or third largest area in cost terms, after instrumentation.
Benchtop Automation Trends 2013
HTStec

This market report summarizes the results of HTStec's industry-wide global web-based benchmarking survey on small-scale or benchtop automation carried out in January 2013.
Next-Gen Sequencing Trends 2012
HTStec

This market report summarizes the results of HTStec’s 3rd industry-wide global web-based benchmarking survey on next-generation sequencing (NGS) carried out in November 2012.
High Throughput qPCR Assay Trends
HTStec

This market report summarizes the results of HTStec’s global web-based benchmarking survey on high throughput real-time quantitative PCR (HT qPCR) assays carried out in January 2012.
The Future of Molecular Diagnostics: Innovative technologies driving market opportunities in personalized medicine
Business Insights

This report analyzes the current and potential world markets for molecular diagnostics, including advances in the field. This report generally forecasts future growth to 2015. The report breaks down market forecasts by type of test and by geography. It indicates which companies have key products in the market place. It also analyzes key drivers of this market.
Scientific News
Promising Compound Rapidly Eliminates Malaria Parasite
An international research collaborative led by St. Jude Children’s Research Hospital reports that the rapid action of (+)-SJ733 will likely slow malaria drug resistance.
Genes that Cause Pancreatic Cancer Identified by New Tool
Screening system in mice spots cancerous changes invisible to sequencing.
Using Genome Sequencing to Track MRSA in Under-resourced Hospitals
Whole genome sequencing of MRSA from a hospital in Asia has demonstrated patterns of transmission in a resource-limited setting, where formal screening procedures are not feasible.
Comprehensive Genomic Study of Sub-Saharan Africans Conducted
New data resource will enhance disease research and genomic diversity studies.
Genetic Errors Linked to More ALS Cases than Scientists had Thought
Number of mutated genes influences the age when the fatal paralyzing disorder first appears.
WormBase ParaSite Launched
Open-access database WormBase ParaSite launches with data on 1.5 million genes.
Big Data Set To Make A Big Difference In Childhood Cancer Treatment
UTS researchers are working with the Kids Research Institute to visualise large quantities of patient data to better diagnose and treat childhood cancer patients.
Two Studies Identify A Detectable, Pre-Cancerous State In The Blood
Findings pave way for new lines of cancer research focused on detection and prevention.
Fast, Low-cost DNA Sequencing Technology One Step Closer To Reality
ASU Regents' Professor Stuart Lindsay led a team of scientists from Arizona State University's Biodesign Institute and IBM's T.J. Watson Research Center in the development of a prototype DNA reader that could make whole genome profiling an everyday practice in medicine.
Amazing Feet Of Science: Researchers Sequence The Centipede Genome
What it lacks in genes, it certainly makes up for in legs: the genome of the humble centipede has been found to have around 15,000 genes – around 7,000 fewer than a human.
Business News
Making Single-Cell Biology Work
New collaboration between EMBL-EBI, the Wellcome Trust Sanger Institute and Fluidigm keeps research and technology on the cutting-edge of single-cell genomics.
14M Genomics and Syncona Sign £12.5m Financing
14M Genomics and Syncona sign £12.5 million financing to develop cancer genomic diagnostics in partnership with the Wellcome Trust Sanger Institute.
RainDance Forms Hematologic Oncology Consortium
HemOnc experts validate unmet need and performance of new ThunderBolts Myeloid NGS Panel at ASH 2014.
New Companies Join Cancer Research UK Consortium
Three new biomarker companies have been selected to work with the Early Diagnosis Consortium, a collaboration between Cancer Research UK, its commercial arm, Cancer Research Technology and Abcodia.
NIH Awards Grant For Malaria Research
Malaria researchers use new genomic tools to individualize parasite analysis.
SYGNIS, BioCat Sign Non-Exclusive Distribution Agreement
Company grants BioCat non-exclusive rights to promote, market and sell TruePrime™ Single Cell WGA kit.
OGT Announces Operational and Financial Highlights for FY 2014
OGT’s molecular genetics business delivers 54% growth in commercial revenues to £11.9m.
Kapa Biosystems European Expansion
Kapa Biosystems strengthens European commercial operations with dedicated service and support for customers in the United Kingdom.
University of Chicago to Establish Genomic Data Commons
First-of-its-kind facility expands access to large-scale cancer genomic data.
Illumina and Sequenom Enter Settlement
Illumina and Sequenom pool noninvasive prenatal testing intellectual property and end outstanding patent disputes.

Events
Biomarkers from Research to Cl
15 Jan 2015
Biomarkers from Research to Cl ...
More Information
RNA-Seq 2015
20 Jan 2015
RNA-Seq 2015 ...
More Information
Lab-on-a-Chip & Microfluidics
17 Mar 2015
Lab-on-a-Chip & Microfluidics ...
More Information
Microarray Technology
17 Mar 2015
Microarray Technology ...
More Information

ePosters
Identifying Molecular Signatures of Tumors Using Novel Fluorescence Resonance Energy Transfer Networks
Duke University

We developed FRET sensors that can detect 125 fluorophores simultaneously. From experimental analyses of over 1200 time-resolved fluorescence signatures on 300 prototypical sensors, we show that the optical responses are highly repeatable and minor variations between FRET networks can be discriminated resulting in a total of 10^375 unique responses in theory.

NEXTflex™ qRNA-Seq™ Molecular Indexing for ChIP-Seq and RNA-Seq
Bioo Scientific

Most Next Generation Sequencing (NGS) library prep methods introduce sequence bias with the use of enzyme processing and fragmentation steps can introduce errors in the form of incorrect sequence and misrepresented copy number. With molecular indexed libraries, each molecule is tagged with a molecular index randomly chosen from ~10,000 combinations so that any two identical molecules become distinguishable (with odds of 10,000/1), and can be independently evaluated in later data analysis.
A complete workflow from sample preparation to analysis using SureSelect target enrichment system for Ion Proton semiconductor sequencing
Agilent Technologies

Agilent’s SureSelect Target Enrichment for the Ion Proton Platform provides a comprehensive, efficient, robust, and cost-effective means to sequence subsets of the human genome.

Genotyping-by-Sequencing of a set of diverse spring barley (Hordeum vulgare) accessions
(1) Julius Kühn-Institute (JKI), Federal Research Centre for Cultivated Plants, Institute for Resistance Research and Stress Tolerance, Erwin-Baur-Str. 27, 06484 Quedlinburg, Germany; (2) Julius Kühn-

The poster presents a Genotyping-by-Sequencing (GBS) approach to saturate a set of diverse spring barley accessions with a high density of SNP markers.
Genotyping-by-Sequencing of a set of diverse spring barley (Hordeum vulgare) accessions
(1) Julius Kühn-Institute (JKI), Federal Research Centre for Cultivated Plants, Institute for Resistance Research and Stress Tolerance, Erwin-Baur-Str. 27, 06484 Quedlinburg, Germany; (2) Julius Kühn-

The poster presents a Genotyping-by-Sequencing (GBS) approach to saturate a set of diverse spring barley accessions with a high density of SNP markers.
Stealth-Adapted Viruses and Viteria: Insights into Virus Construction, Replication and Potential Therapies
Institute of Progressive Medicine

There is an increasing incidence of diseases with accompanying signs and symptoms of brain damage. These include neurological and psychiatric illnesses, childhood behavioral disorders, and such common conditions as chronic fatigue, Gulf War Syndrome, so-called “chronic Lyme disease”, and many cancers. Altogether, these diseases have an enormous social impact.
High-Throughput Analysis of DNA Samples using the D1K ScreenTape Assay and the Agilent 2200 TapeStation System
Agilent Technologies

Recent advances in genomics demands to look at a wealth of genetic information in a short period of time. DNA analysis using slab gel electrophoresis and capillary electrophoresis are widely being used as a QC step in next generation sequencing and microarray studies. However, often these techniques lack the speed and involve more manual steps to perform the assay.
Improved Ligation Specificity with Chemically Modified Ligation Components
TriLink BioTechnologies, Inc.

Ligases are gaining utility in molecular biology applications, such as nucleotide sequence detection, single nucleotide polymorphism (SNP) detection, protein detection and “next generation” sequencing by ligation.
Defining off-target cleavage in a pair of Zinc Finger Nucleases
University of Utah

This study looks at off-target cleavage of Zinc Finger Nucleases (ZNFs) in Drosophila in an attempt to analyze potential cleavage spots, with a view to designing more efficient ZFNs.
Efficacy of Using a Combination Microplate Washer for Vacuum-Based DNA Sequencing Reaction Cleanup
BioTek Instruments

The ability to determine the specific pattern of base pairs in DNA molecules is an indispensable part of contemporary molecular biology. This poster demonstrates how the vacuum filtration module available on the BioTek 405 Touch effectively cleans contaminating artifacts from DNA sequencing reactions, which wil contribute to the genomic workflow typical of many molecular biology laboratories and core facilities.
Application Notes
Approaching Single-Cell Sequencing by Understanding NGS Library Complexity and Bias
Swift Biosciences Inc

This application note describes the sequence coverage performance andp reservation of molecular complexity of next generation sequencing (NGS) libraries generated from human and microbial genomic DNA using the Accel-NGS™ 2S DNA Library Kit for whole-genome sequencing (WGS) on the Illumina® platform.
Automated Illumina TruSeq® Nano DNA Sample Preparation
Tecan Group Ltd.

The TruSeq Nano DNA Sample Preparation Kit enables efficient investigation of samples where the amount of DNA available is limited. This low-input method generates excellent quality data from as little at 100 ng of DNA, allowing the study of samples with restricted DNA availability, for example from tumors, and helping to preserve precious sample material for use in future studies.
Double stranded DNA quantitation using Promega QuantiFluor® Dyes
Tecan Group Ltd.

Achieving selective and sensitive quantitation of double stranded DNA (dsDNA) using spectrophotometric methods can be problematic. Recognizing these difficulties, researchers have incorporated fluorescence-based quantitation techniques in their workflows to obtain the required sensitivity and target selectivity.
Automated Illumina NGS Sample Preparation: TruSeq® Stranded mRNA on the Hamilton Microlab STAR
Hamilton Robotics

Illumina’s sample preparation kits produce high quality libraries for DNA and RNA sequencing. Hamilton offers the robust Microlab STAR line of robotic liquid handling workstations for automating sample preparation. Together, the automation of the TruSeq Stranded mRNA sample preparation protocol on the STAR gives the user hours of reliable, unattended library preparation.
Molecular indexing for improved RNA-Seq analysis
Bioo Scientific

Most modern methods for nucleic acid analysis require the use of enzyme processing, such as DNA polymerase reactions, in the sample preparation or measurement steps. Although necessary, these enzymatic steps introduce errors in the form of incorrect sequence and misrepresented copy number. NEXTflex™ qRNA-Seq™ Kit from Bioo Scientific adds no additional steps to the work flow, costs no more than a conventional library preparation kit and increases the precision of downstream analysis.
An Integrated Solution to Simplify Library Preparation and Multiplexing for NimbleGen Sequence Capture
Bioo Scientific

The studies described herein demonstrate the capability of Bioo Scientific’s novel NEXTFlex™ DNA Pre-Capture Combo Library construction kits used in conjunction with the NimbleGen Sequence Capture technology to obtain high coverage comparative genomic data from a panel of human HapMap DNA samples.
Next Generation Sequencing Method for Illumina TruSeq DNA Sample Preparation Protocol on the Hamilton STAR
Hamilton

The purpose of the TruSeq sample preparation protocol is to add adapter sequences onto the ends of DNA fragments to generate multiplexed sequencing libraries. Good liquid handling is crucial for pipetting precise volumes for example for Agilent Bioanalyzer.
Tools for Enhancing Sequence Diversity and Reducing Bias in DNA-seq Library Preparation
Bioo Scientific

The generation of high quality next generation sequencing data begins with libraries that have the desired insert size and proper adapter ligation.
Sequence-Specific DNA Assay
Molecular Devices

The Threshold® system from Molecular Devices, together with the Immuno Ligand Assay (ILA) kit can be used to measure specific DNA sequences. Biotinylated or fluorescienated oligonucleotide probes, specific to the target sequence, anneal to the digested target DNA creating a probe-target hybrid which can then be detected and quantified against a standard curve, with pleasing results.
High Yield and Quality with Thermo Scientific Microtiter Deep Well 96 plates in KingFisher Flex Process
Thermo Fisher Scientific

The Thermo Scientific KingFisher Flex magnetic particle processor is specifically designed to automate the time-consuming sample preparation of proteins, nucleic acids and cells in 96-well plate formats.
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