Corporate Banner
Satellite Banner
Next Gen Sequencing
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

Life Technologies Launches Two Ion AmpliSeq™ Community Panel Designs

Published: Wednesday, April 03, 2013
Last Updated: Wednesday, April 03, 2013
Bookmark and Share
Company has launched Ion AmpliSeq™ BRCA1 and BRCA2 Panel and the Ion AmpliSeq™ Colon & Lung Cancer Panel.

Life Technologies Corporation has announced that it has launched two Ion AmpliSeq™ Community Panels: the Ion AmpliSeq™ BRCA1 and BRCA2 Panel design and the Ion AmpliSeq™ Colon and Lung Cancer Panel design.

The BRCA panel analyzes the coding regions of the tumor suppressor genes BRCA1 and BRCA2, which have been implicated in hereditary breast and ovarian cancers.

The colon and lung panel analyzes more than 500 mutations in 22 associated genes, using the power and simplicity of the established Ion AmpliSeq™ technology.

Significant and ongoing improvements with Ion sequencing have enabled these Ion AmpliSeq™ panels to accurately call all variants of interest, including those within difficult sequence contexts.

Life Technologies is hosting three talks at the American Association of Cancer Research (AACR) on Saturday, April 6 about the use of Ion AmpliSeq™ panels in clinical research:

• Nicola Normanno, MD, Centro Ricerche Oncologiche di Mercogliano, Avellino, Italy, will discuss the development of the Ion AmpliSeq™ Lung and Colon Community Panel and the collaborative efforts of the eight-member European consortia.
• Marina Nikiforova, MD, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania will discuss using the Ion AmpliSeq™ RNA Panels for detection of chromosomal rearrangements in thyroid cancer research.
• Six panelists will discuss implementing Ion AmpliSeq™ Cancer Gene Panels on the Ion PGM™ System for clinical research.

Ion AmpliSeq™ Community Panels are developed by leading scientists who draw on their expertise in a specific disease area to develop the panels.

They verify the content using samples that were previously screened using orthogonal technologies. The panel designs are then made available to the entire scientific community on ampliseq.com.

Ion AmpliSeq™ Community Panels are built on the same breakthrough technology that powers the Ion AmpliSeq™ Ready-to-Use Panels and the Ion AmpliSeq™ Custom Panels.

Like all Ion AmpliSeq™ technology, the community panels require just 10 ng of DNA input per primer pool, about 25-fold less than alternative gene panel approaches.

The Ion AmpliSeq™ workflow is a simple PCR reaction coupled with rapid Ion semiconductor sequencing, enabling researchers to go from DNA to results in less than 24 hours.

The Ion AmpliSeq™ BRCA1 and BRCA2 panels were developed by Drs. Marjolijn Ligtenberg and Arjen Mensenkamp from Radboud University Nijmegen Medical Centre, The Netherlands, and with Drs. Jose Carlos Machado and Jose Luis Costa from The Institute of Molecular Pathology and Immunology of the University of Porto.

In addition to these leading researchers in the field, a global consortium will further test the panel, with plans to validate and implement it in their labs.

"Ion AmpliSeq™ technology has allowed us to substantially improve the turnaround time and cost of sequencing these important genes," said Costa. "The methodology proved robust enough to call even difficult mutations, including long homopolymers, in these two genes."

"The Ion AmpliSeq™ BRCA panel enabled us to develop a quick and easy high-throughput workflow, with minimal hands-on time," said Mensenkamp. "It is an accurate solution-of the 65 samples tested to date, we detected all expected mutations."

Life Technologies has announced the community panel program in November with the first Ion AmpliSeq™ Community Panel-the Ion AmpliSeq™ Colon and Lung Cancer Panel design.

The panel analyzes more than 500 mutations in a single-tube assay that requires just 10 ng of DNA input per primer pool, about 25-fold less than alternative gene panel approaches.

The Ion AmpliSeq™ workflow is as simple as a PCR reaction and can be completed in as few as 3.5 hours turnaround time. That panel design is now available on ampliseq.com.

"The Ion AmpliSeq™ Colon and Lung Cancer panel design permits you to describe a cancer according to the molecular subpopulation that compose the lesion and this is impossible to do with previous methods," said Dr. Aldo Scarpa, Director, Dept. of Pathology and ARC-NET Research Centre for Applied Research on Cancer, University of Verona, Italy.

Eight institutions - dubbed the OncoNetwork - participated in the development of the panel: Centro Ricerche Oncologiche Mercogliano, Italy; Leiter Genetik / Molekularbiologie Viollier AG, Switzerland; University of Warwick, UK; Institut Gustave Roussy France; Radboud University Nijmegen Medical Centre, The Netherlands; Universite Paris Descartes Centre, Universitaire des Saints-Peres, France; St James' Hospital, Dublin EIRE and ARC-NET Research Center, University of Verona, Italy.

The Ion AmpliSeq™ panels referenced are For Research Use Only. Not for use in diagnostic procedures.


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 3,300+ scientific posters on ePosters
  • More than 4,800+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Life Technologies to Collaborate with Boston Children's Hospital
Collaboration to develop an optimized lab workflow based on the Ion Proton™ sequencer.
Thursday, June 21, 2012
Life Technologies Establishes Global Instrument Center of Excellence in Singapore
Center will design and manufacture Company’s products, including next-generation sequencing and molecular diagnostic instruments.
Friday, June 15, 2012
Scientific News
Decoding the Genome of the Olive Tree
A team of scientists from three Spanish centers has sequenced, for the first time ever, the complete genome of the olive tree. This work will facilitate genetic improvement for production of olives and olive oil, two key products in the Spanish economy and diet.
Four Newly-Identified Genes Could Improve Rice
A Japanese research team have applied a method used in human genetic analysis to rice and rapidly discovered four new genes that are potentially significant for agriculture. These findings could influence crop breeding and help combat food shortages caused by a growing population.
What Makes a Good Scientist?
It’s the journey, not just the destination that counts as a scientist when conducting research.
Biomarkers That Could Help Give Cancer Patients Better Survival Estimates Discovered
UCLA research may also help scientists suppress dangerous genetic sequences.
Mobile Laboratories Help Track Zika Spread Across Brazil
Researchers from the University of Birmingham are working with health partners in Brazil to combat the spread of Zika virus by deploying a pair of mobile DNA sequencing laboratories on a medical ‘road trip’ through the worst-hit areas of the country.
How “Silent” Genetic Changes Drive Cancer
The researchers found that EXOSC2 expression is enhanced in metastatic tumors because their cells have increased levels of a tRNA called GluUUC.
‘Jumping Gene’ Took Peppered Moths To The Dark Side
Researchers from the University of Liverpool have identified and dated the genetic mutation that gave rise to the black form of the peppered moth, which spread rapidly during Britain’s Industrial Revolution.
Benchtop Automation Trends
Gain a better understanding of current interest in and future deployment of benchtop automated systems.
How Did The Giraffe Get Its Long Neck?
Clues about the evolution of the giraffe’s long neck have now been revealed by new genome sequencing.
Big Data Can Save Lives
The sharing of genetic information from millions of cancer patients around the world could be key to revolutionising cancer prevention and care, according to a leading cancer expert from Queen's University Belfast.
SELECTBIO

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,300+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,800+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!