Satellite Banner
Next Gen Sequencing
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

Bioo Scientific Launches First Kit For Molecular Indexing in RNA-Seq

Published: Tuesday, May 07, 2013
Last Updated: Tuesday, May 07, 2013
Bookmark and Share
Bioo Scientific introduces the NEXTflex™ qRNA-Seq™ Kit for high precision gene expression analysis by RNA-Seq.

Developed in conjunction with Cellular Research Inc., this new kit efficiently generates libraries equivalent to conventional RNA-Seq libraries, but with the added feature of Molecular Indexing™ technology. Similar to conventional RNA-Seq, sample RNA is converted to cDNA fragments.  But prior to any PCR amplification steps, all DNA fragment ends are ligated to a pair of adaptors chosen at random from a total set of 9,216 molecular indices. Individual DNA molecules of identical sequence become distinct through indexing, allowing for differentiation between re-sampling of the same molecule and sampling of a different molecule of identical sequence. Analysis using molecular indexing information provides an absolute, digital measurement of gene expression levels, irrespective of common amplification distortions observed in many RNA-Seq experiments.

"We are pleased to introduce the first in a series of high-precision assays from Cellular Research Inc., enabling a new level of accuracy for identifying and counting distinct DNA molecules.", said Stephen Fodor, PhD, CEO of Cellular Research, Inc. “Bioo Scientific’s reputation for building high quality RNA Sequencing kits with strong customer service and support make them an ideal commercialization partner.”

All of the NEXTflex™ kits incorporate the proprietary “Enhanced Adapter Ligation Technology”, which offers the highest ligation efficiency available, resulting in longer and more diverse sequencing reads. Bioo Scientific’s robust enzymes ensure the highest quality libraries for global RNA profiling.


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 3,000+ scientific posters on ePosters
  • More than 4,500+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Bioo Scientific Awarded $550K to Improve Single Cell Sequencing Technologies
The first grant, Sequencing of Epigenetic Modifications from Single Cells, will be used to further the development of single-cell methylome sequencing for use in a number of applications.
Monday, April 11, 2016
Bioo Scientific Granted Patent
Company has announced that the USPTO has issued U.S. patent for improving data quality and small RNA sequencing technology.
Wednesday, March 16, 2016
Bioo Scientific Partners with the Icahn School of Medicine at Mount Sinai
Partnership to reduce ligation bias in next generation sequencing.
Saturday, February 14, 2015
Bioo Scientific and Icahn School of Medicine Partner
Bioo Scientific partners with the Icahn School of Medicine at Mount Sinai to reduce ligation bias in next generation sequencing.
Tuesday, February 10, 2015
Bioo Scientific Awarded $750,000 NSF Grant
Company awarded a National Science Foundation Phase II grant to continue development efforts to improve the accuracy of small RNA expression analysis.
Wednesday, November 26, 2014
Bioo Scientific Receives Presidential Award for Exports
Awards are the highest recognition any U.S. entity may receive for making a significant contribution to the expansion of U.S. exports.
Thursday, May 29, 2014
Scientific News
Monovar Drills Down Into Cancer Genome
Rice, MD Anderson develop program to ID mutations in single cancer cells.
Five New Breast Cancer Genes Found
Discovery of mutations paves the way for personalised treatment of breast cancer.
New Neurodevelopmental Syndrome Identified
Study pinpoints underlying genetic mutations, raising hopes for targeted therapies.
Uncovering Hidden Genomic Alterations that Drive Cancers
Tested on large tumor genomics database, REVEALER method allows researchers to connect genomics to cell function.
Gene Behind Rare Childhood Syndrome Identified
Online activism by one patient’s mother spurred research collaboration which led to the identification of a new genetic syndrome.
Resilience Project Identifies Rare Unaffected Individuals
Researchers from Mount Sinai and Sage Bionetworks report analysis of nearly 600,000 genomes for resilience project.
Rare DNA Will Have Nowhere To Hide
Two National Institutes of Health grants back Rice University effort to develop new diagnostics.
Virus Causing Tilapia Die-Offs Identified
Discovery of the virus causing Tilapia die-offs in Israel and Ecuador points the way to protecting a fish that feeds multitudes.
Children With Cancer To Get New Gene Test
Pilot study will sequence 81 cancer genes in children’s tumours to help personalise cancer treatment.
How The Bat Got Its Wings
Finding may provide clues to human limb development and malformations.
Skyscraper Banner

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,000+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,500+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!