Corporate Banner
Satellite Banner
Next Gen Sequencing
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

Modifying the Oligo Backbone - Advice for PNA and UNA Chemistry

Published: Tuesday, December 10, 2013
Last Updated: Monday, December 09, 2013
Bookmark and Share
Link Technologies provides key protocols for oligonucleotide synthesis and modification.

Link Technologies Ltd is providing scientists with a complete resource for oligonucleotide synthesis and modification.

Spanning the breadth of oligo chemistry, the Link Technologies 2013/14 Product Guide leads users through practical methods and application-based advice for total assurance in reagent selection and performance of the resulting oligos.

Featuring specialist sections, including backbone modification strategies using Peptide Nucleic Acid (PNA) and Unlocked Nucleic Acid (UNA) reagents, the guide provides scientists with the information required to produce oligos that are suitable for both routine and highly specific application tasks. The resource is free to request as a hard copy or to download from www.linktech.co.uk.

Modification of the sugar-phosphate backbone with nucleic acid derivatives, including PNA and UNA, alters the structure and subsequent properties of the oligo. For antisense applications this is often favourable as PNA oligos, for example, display tighter binding to complementary DNA and RNA sequences, and with greater specificity.

In this way, PNA-modified oligos can provide a flexible tool for use in genetic diagnostics, and can also be applied more broadly to techniques such as in situ hybridization and PCR clamping. Contrastingly UNA modifications act to decrease the binding affinity with complementary strands, and so UNA incorporation has been exploited in siRNA-based silencing technology for example, to protect the siRNA from serum degradation without compromising potency.

For scientists looking to take advantage of these modified properties, the Link Technologies Product Guide collates referenced background information, established protocols and application notes, enabling the successful and confident synthesis of such oligos.

Featuring over 80 pages of protocols and quick-reference data summaries, the 2013/14 Product Guide has been produced by Link Technologies to provide invaluable technical and functional support for lab scientists using nucleic acid chemistry.

Amy Barker, Research Technician at the University of Leeds, explains: “My research group is concerned with developing RNA aptamers against various biological targets, and so in my role, keeping up to date with the latest advancements in nucleic acid modifications and reagents is vital. For me, the Link Technologies Product Guide is extremely useful - especially the protocols section - but the handy working examples and further external referencing are also an easy port-of-call when I’m required to synthesize new oligos.”


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 3,500+ scientific posters on ePosters
  • More than 5,000+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.


Scientific News
NASA's DNA Sequencing in Space is a Success
DNA has been sequenced in space for the first time ever for the Biomolecule Sequencer investigation, using the MinION sequencing device.
New Inflammatory Disease Discovered
NIH researchers have discovered a rare and potentially deadly disease - otulipenia - the mostly affects children.
Bringing NGS to the Crime Lab
New technology being validated in BCI lab for use in Ohio missing persons cases.
Expanding Knowledge of Viral Diversity
Environmental datasets help researchers double the number of microbial phyla known to be infected by viruses.
How Cloud Connectivity Can Combat the Reproducibility Crisis
This infographic explains the reproducibility crisis, and how cloud connectivity can help overcome this problem.
The Power of Model Systems
New insights into the influence of host on the gut microbiome are revealed with in situ light sheet fluorescence microscopy and stochastic mathematical modelling.
Mapping Antibody Creation in Humans
Researchers have created the first, detailed map of the body's antibody production, which could suggest new treatment options for immune disorders.
New Way To Measure Important Chemical Modification On RNA
Technology could advance stem cells’ use in regenerative medicine, UCLA researchers say.
Decoding the Genome of the Olive Tree
A team of scientists from three Spanish centers has sequenced, for the first time ever, the complete genome of the olive tree. This work will facilitate genetic improvement for production of olives and olive oil, two key products in the Spanish economy and diet.
Four Newly-Identified Genes Could Improve Rice
A Japanese research team have applied a method used in human genetic analysis to rice and rapidly discovered four new genes that are potentially significant for agriculture. These findings could influence crop breeding and help combat food shortages caused by a growing population.
Skyscraper Banner

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,500+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
5,000+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!