Corporate Banner
Satellite Banner
Next Gen Sequencing
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

Enhanced Molecular Inversion Probes for NGS Target Enrichment

Published: Tuesday, February 04, 2014
Last Updated: Tuesday, February 04, 2014
Bookmark and Share
IDT presents latest developments at 15th annual Advances in Genome Biology and Technology meeting.

Integrated DNA Technologies (IDT) will present its findings on the use of molecular inversion probes (MIPs) for next generation sequencing (NGS) target enrichment at the 15th annual Advances in Genome Biology and Technology (AGBT) meeting in Marco Island, Florida, Feb 12-15.

John Havens PhD, Vice-President of Business Development at IDT, will talk about MIPs and their proven effectiveness in the enrichment of genomic content of biological samples for specific regions of interest. NGS can subsequently be used to characterize these regions at high coverage depths.

In comparison to other methods, MIPs provide high multiplexability of targets in a single tube, which combined with the ability to enrich small amounts of DNA, results in time- and cost-effective sequencing.

IDT has developed a procedure whereby a high-fidelity platform is used to synthesize MIPs as long as 200 bases, chemically phosphorylating them independent of sequence. The presence of full length MIPs is then verified using mass spectroscopy prior to pooling.

Barcodes can be directly incorporated via degenerate bases and used to characterize the complexity of target capture. Dr Havens will demonstrate the advantage of these MIPs in terms of target specificity and the capture of longer targets.

A direct comparison will also be given of this approach with hybridization for a set of oncology targets, highlighting the benefits of MIPs over more traditional methods.


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 3,000+ scientific posters on ePosters
  • More than 4,400+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

TBUSA, IDT Announce Collaboration
Collaboration aims to support targeted RNA Sequencing.
Wednesday, April 27, 2016
Fast and Focused Sequencing from Single Genes to Entire Exomes
IDT reveals how to increase sequencing success with new and existing enrichment panels.
Monday, March 16, 2015
Discover Affordable Custom NGS Panels
IDT webinar helps NGS researchers bring cost-savings to target capture panels.
Thursday, February 12, 2015
Discover the Latest NGS-Based Assays Guiding Precision Oncology
IDT joins forces with Foundation Medicine in AMP workshop.
Saturday, November 08, 2014
IDT Shares Tips for Optimizing Target Capture
The article discusses improved target capture methods for increasing reliability of next generation sequencing data.
Friday, October 24, 2014
From Honey Bees to Hearts - Keep Track of the Latest in NGS
Free IDT symposium explores diverse NGS applications.
Wednesday, September 24, 2014
Not a Constant Value: Understanding Tm
IDT explains how to optimize oligo hybridization-based protocols.
Friday, December 20, 2013
Bringing NGS to the Clinic: What Can We Expect?
Dr Elaine Mardis shares her views with IDT on the future of NGS and its role in personalized medicine.
Thursday, February 07, 2013
Integrated DNA Technologies Acquires 1st BASE Oligonucleotide Business in Singapore
Already the world leader in custom nucleic acid synthesis, IDT will now offer locally-manufactured oligos to researchers in Singapore and throughout the region from the company’s first synthesis facility in Asia.
Thursday, February 09, 2012
Scientific News
New Neurodevelopmental Syndrome Identified
Study pinpoints underlying genetic mutations, raising hopes for targeted therapies.
Uncovering Hidden Genomic Alterations that Drive Cancers
Tested on large tumor genomics database, REVEALER method allows researchers to connect genomics to cell function.
Gene Behind Rare Childhood Syndrome Identified
Online activism by one patient’s mother spurred research collaboration which led to the identification of a new genetic syndrome.
Resilience Project Identifies Rare Unaffected Individuals
Researchers from Mount Sinai and Sage Bionetworks report analysis of nearly 600,000 genomes for resilience project.
Rare DNA Will Have Nowhere To Hide
Two National Institutes of Health grants back Rice University effort to develop new diagnostics.
Virus Causing Tilapia Die-Offs Identified
Discovery of the virus causing Tilapia die-offs in Israel and Ecuador points the way to protecting a fish that feeds multitudes.
Children With Cancer To Get New Gene Test
Pilot study will sequence 81 cancer genes in children’s tumours to help personalise cancer treatment.
How The Bat Got Its Wings
Finding may provide clues to human limb development and malformations.
Lupus Study Shows Precision Medicine’s Potential to Define the Genetics of Autoimmune Disease
Researchers at UT Southwestern have used next-generation DNA sequencing technology to identify more than 1,000 gene variants that affect susceptibility to SLE.
Illuminating the Broad Spectrum of Disease
PRISM efficiently tests drug compounds in multiple cell lines simultaneously, accelerating discovery of targeted therapies in the service of precision medicine.
Skyscraper Banner

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,000+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,400+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!